Citation: WANG Wei, HUANG Xianhui, WANG Hui, YAN Changyan, KONG Xiangkai. Determination of phenolic and salicylanilide anthelmintics in liquid milk by high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2013, 31(10): 1028-1032. doi: 10.3724/SP.J.1123.2013.03006 shu

Determination of phenolic and salicylanilide anthelmintics in liquid milk by high performance liquid chromatography

1.
National Reference Laboratory of Veterinary Drug Residues, South China Agricultural University, Guangzhou 510642, China

Corresponding author: HUANG Xianhui,
Received Date: 1 March 2013
Available Online: 20 April 2013
Fund Project: 2012年农业行业标准制修订项目(201214148). (201214148)

An analytical method for the determination of nitroxynil, oxyclozanide, closantel and rafoxanide in liquid milk by high performance liquid chromatography (HPLC) has been established. The milk sample was extracted with acetonitrile containing 1% (v/v) triethylamine. The supernatant was purified by an anion exchange solid phase extraction column. The analyte was detected by ultraviolet detector after the HPLC separation on a C18 RP column. The mobile phase was composed of acetonitrile-0.02 mol/L ammonium acetate solution with pH 4.0. The linear ranges of the four drugs in the spiked blank milk samples were 5-500 μg/kg, and the correlation coefficients were higher than 0.99. The limits of detection (LOD) were 3 μg/kg, and the limits of quantification (LOQ) were 5 μg/kg. The average recoveries and relative standard deviations (RSDs) of nitroxynil, oxyclozanide, closantel and rafoxanide at the spiked levels of 1/2MRL (maximum residue limit), MRL and 2MRL ranged from 92.20% to 96.13%, 5.55% to 16.30%; 87.40% to 94.74%, 5.40% to 12.21%; 86.97% to 91.09%, 2.67% to 8.17%; and 77.86% to 95.36%, 5.02% to 13.15% respectively. The method is simple and sensitive for the quantification of phenolic and salicylanilide anthelmintics in liquid milk.
- high performance liquid chromatography (HPLC),
- nitroxynil,
- oxyclozanide,
- closantel,
- rafoxanide,
- milk,
- multiresidue
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沈阳化工大学材料科学与工程学院沈阳 110142