Citation: HUANG Lanqi, ZHOU Weihua, CHEN Yiwang, ZHANG Lingyi, WAN Yiqun, ZHANG Weibing. Separation performance of vancomycin-bonded stationary phase[J]. Chinese Journal of Chromatography, ;2013, 31(9): 821-824. doi: 10.3724/SP.J.1123.2013.02003 shu

Separation performance of vancomycin-bonded stationary phase

1.
1. Department of Chemistry, Nanchang University, Nanchang 330031, China;

Corresponding author: ZHOU Weihua,
Received Date: 4 February 2013
Available Online: 3 March 2013
Fund Project: 食品科学与技术国家重点实验室2010年度开放基金(SKLF-KF-201002). (SKLF-KF-201002)

Vancomycin is one of the macrocyclic antibiotics with complex molecular structure. Based on the various functional groups of vancomycin, a vancomycin-bonded stationary phase using glutaraldehyde as the spacer was prepared. Its chromatographic properties in reversed-phase, ion exchange and hydrophilic phase modes were investigated separately. The prepared stationary phase showed the typical characteristic of the reversed-phase stationary, when the organic solvent content was low in the mobile phase. On the contrary, its chromatographic characteristic transformed into hydrophilic phase mode with the organic solvent content increased in the mobile phase. Owing to the amino groups of vancomycin, ion exchange mode can also be applied to the separation method development. The vancomycin-bonded stationary phase was applied to the separation of eight achiral drugs and stevioside in reversed-phase, ion exchange and hydrophilic phase modes. The separations were achieved in three different kinds of separation modes by using appropriate chromatographic conditions. The results provide guidance for the design of new types of stationary phase, and method development of chromatographic stationary phases modified by special compounds with complex construction in corresponding separation modes.
- vancomycin,
- stationary phase,
- separation mode
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