Citation: WANG Jing, WANG Dan, ZHANG Hua, PENG Xiaojun, DONG Junpu. Analysis of docosahexenoic acid in human blood using heterocyclic derivatization-gas chromatography-triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, ;2013, 31(8): 734-738. doi: 10.3724/SP.J.1123.2013.01050 shu

Analysis of docosahexenoic acid in human blood using heterocyclic derivatization-gas chromatography-triple quadrupole mass spectrometry

WANG Jing ¹ ,
WANG Dan ² ,
ZHANG Hua ^1,, ,
PENG Xiaojun ¹ ,
DONG Junpu ²

1.
1. State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China;

Corresponding author: ZHANG Hua,
Received Date: 30 January 2013
Available Online: 17 March 2013

A method was developed and validated for the analysis of docosahexenoic acid (DHA) in human blood by heterocyclic derivatization-gas chromatography coupled with triple quadrupole mass spectrometry (GC-MS/MS). 2-Amino-2-methyl-1-propanol (AMP) was used as the reaction reagent of DHA heterocyclic derivatization and the most optimal reaction conditions of this reaction were optimized. Multiple reaction monitoring and internal standard calibration curve were applied to detect DHA by GC-MS/MS. The linear range for the determination of DHA was 0.07-10 μg/mL (r²=0.9991). The limit of detection (S/N=2.8) was 0.02 μg/mL and the limit of quantification (S/N=10) was 0.07 μg/mL. The average recoveries of DHA at three spiked levels of 0.5, 1.5 and 2.5 μg ranged from 94.40% to 103.13% and the relative standard deviations (RSD) were in the range of 1.51%-3.16%. The method was simple, accurate, reliable and small amount of sample was required. It was suitable for detecting the contents of DHA in human blood.
1. [1]
  [1] Zhu L N, Zhang Z G, Zhang M, et al. China Dairy Industry (朱丽娜, 张志国, 张敏, 等. 中国乳品工业), 2009, 37(2): 45
2. [2]
  [2] Wang Y N, Hou D Y, Xin G, et al. Journal of Anshan Normal University (王月囡, 侯冬岩, 辛广, 等. 鞍山师范学院学报), 2012, 14(4): 50
3. [3]
  [3] Luo H, Huang B, Wang J, et al. Nursing Practice and Research (罗红, 黄冰, 王静, 等. 护理实践与研究), 2011, 8(2): 73
4. [4]
  [4] Guo Y H, Li Y J. Meat Research (郭玉华, 李钰金. 肉类研究), 2011(1): 82
5. [5]
  [5] National Postdoctoral Workstation Group of Xiuzheng Pharmaceutical Group Co. Chinese Journal of Healthy Birth & Child Care (修正国家级博士后工作站专家组. 中国优生优育), 2010, 16(4): 219
6. [6]
  [6] Cao W X, Meng J, Tian Y X. China Oils and Fats (曹万新, 孟橘, 田玉霞. 中国油脂), 2011, 36(3): 1
7. [7]
  [7] Bailey-Hall E, Nelson E B, Ryan A S. Lipids, 2008, 43(2): 181
8. [8]
  [8] Petrovic M, Kezic N, Bolanca V. Food Chemistry, 2010, 122(1): 285
9. [9]
  [9] Kangania C O, Kelleyb D E, DeLanya J P. J Chromatogr B, 2008, 873(1): 95
10. [10]
  [10] Tang S, Qin C, Wang H, et al. J Supercrit Fluids, 2011, 57(1): 44
11. [11]
  [11] Loukasa V, Dimizasa C, Sinanogloub V J, et al. Chem Phys Lipids, 2010, 163(3): 292
12. [12]
  [12] Bo H B, Wang X, Zhai Z D, et al. Chinese Journal of Chromatography (薄海波, 王霞, 翟宗德, 等. 色谱), 2006, 24(2): 181
13. [13]
  [13] Kou X Y, Yu G P. Food Research and Development (寇秀颖, 于国萍. 食品研究与开发), 2005, 26(2): 46
14. [14]
  [14] Li Q C, Zhang C, Peng H M, et al. Acta Pharmaceutica Sinica (李青翠, 张驰, 彭会明, 等. 药学学报), 1997, 32(8): 635
15. [15]
  [15] Xu H, Pan C P. Modem Scientific Instruments (许华, 潘灿平. 现代科学仪器), 2009(2): 116
16. [16]
  [16] Xu H Z, Zhang W L, Chen R G. Acta Nutrimenta Sinica (徐惠珠, 张伟利, 陈瑞冠. 营养学报), 1986, 8(2): 165
17. [17]
  [17] Li Y Y, Zhuang H Q, Mo L E, et al. Journal of Guangdong Medical College (黎运源, 庄海旗, 莫丽儿, 等. 广东医学院学报), 1994, 12(2): 90
18. [18]
  [18] Sun S L, Gu X H, Tang J, et al. Journal of Food Science and Biotechnology (孙素玲, 顾晓红, 汤坚, 等. 食品与生物技术学报), 2005, 24(2): 107
19. [19]
  [19] Zou Y H. Food Science (邹耀洪. 食品科学), 2007, 28(6): 293
20. [20]
  [20] Zhuang W, McKague B, Reeve D. Int J Mass Spectrom, 2004, 232(2): 127
1. [1]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
2. [2]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
3. [3]
  Mengyang LI , Hao XU , Zhonghao NIU , Chunhua GONG , Weihui ZHONG , Jingli XIE . Highly effective catalytic synthesis of β-amino alcohols by using viologen-polyoxometalate hybrid materials. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1294-1300. doi: 10.11862/CJIC.20250080
4. [4]
  Tianlong Zhang , Rongling Zhang , Hongsheng Tang , Yan Li , Hua Li . Online Monitoring and Mechanistic Analysis of 3,5-diamino-1,2,4-triazole (DAT) Synthesis via Raman Spectroscopy: A Recommendation for a Comprehensive Instrumental Analysis Experiment. University Chemistry, 2024, 39(6): 303-311. doi: 10.3866/PKU.DXHX202312006
5. [5]
  Zihan Cheng , Kai Jiang , Jun Jiang , Henggang Wang , Hengwei Lin . Achieving thermal-stimulus-responsive dynamic afterglow from carbon dots by singlet-triplet energy gap engineering through covalent fixation. Acta Physico-Chimica Sinica, 2026, 42(2): 100169-0. doi: 10.1016/j.actphy.2025.100169
6. [6]
  Guojie Xu , Fang Yu , Yunxia Wang , Meng Sun . Introduction to Metal-Catalyzed β-Carbon Elimination Reaction of Cyclopropenones. University Chemistry, 2024, 39(8): 169-173. doi: 10.3866/PKU.DXHX202401060
7. [7]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
8. [8]
  Yan'e LIU , Shengli JIA , Yifan JIANG , Qinghua ZHAO , Yi LI , Xinshu CHANG . MoO₃/cellulose derived carbon aerogel: Fabrication and performance as cathode for lithium-sulfur battery. Chinese Journal of Inorganic Chemistry, 2025, 41(8): 1565-1573. doi: 10.11862/CJIC.20250054
9. [9]
  Chi Li , Jichao Wan , Qiyu Long , Hui Lv , Ying Xiong . N-Heterocyclic Carbene (NHC)-Catalyzed Amidation of Aldehydes with Nitroso Compounds. University Chemistry, 2024, 39(5): 388-395. doi: 10.3866/PKU.DXHX202312016
10. [10]
  Yujie WANG , Laobang WANG , Zheng ZHANG , Qi LIU , Jianping LANG . Construction of W/Cu/S cluster-based supramolecular compounds via alkynyl/sulfur cycloaddition and their third-order nonlinear optical properties. Chinese Journal of Inorganic Chemistry, 2025, 41(10): 2069-2077. doi: 10.11862/CJIC.20250129
11. [11]
  Conghao Shi , Ranran Wang , Juli Jiang , Leyong Wang . The Illustration on Stereoisomers of Macrocycles Containing Multiple Chiral Centers via Tröger Base-based Macrocycles. University Chemistry, 2024, 39(7): 394-397. doi: 10.3866/PKU.DXHX202311034
12. [12]
  Lingbang Qiu , Jiangmin Jiang , Libo Wang , Lang Bai , Fei Zhou , Gaoyu Zhou , Quanchao Zhuang , Yanhua Cui . In Situ Electrochemical Impedance Spectroscopy Monitoring of the High-Temperature Double-Discharge Mechanism of Nb₁₂WO₃₃ Cathode Material for Long-Life Thermal Batteries. Acta Physico-Chimica Sinica, 2025, 41(5): 100040-0. doi: 10.1016/j.actphy.2024.100040
13. [13]
  Gaofeng Zeng , Shuyu Liu , Manle Jiang , Yu Wang , Ping Xu , Lei Wang . Micro/Nanorobots for Pollution Detection and Toxic Removal. University Chemistry, 2024, 39(9): 229-234. doi: 10.12461/PKU.DXHX202311055
14. [14]
  Yuanchun Pan , Xinyun Lin , Leyi Yang , Wenya Hu , Dekui Song , Nan Liu . Artificial Intelligence Science Practice: Preparation of Electronic Skin by Chemical Vapor Deposition of Graphene. University Chemistry, 2025, 40(11): 272-280. doi: 10.12461/PKU.DXHX202412052
15. [15]
  Zhuoyan Lv , Yangming Ding , Leilei Kang , Lin Li , Xiao Yan Liu , Aiqin Wang , Tao Zhang . Light-Enhanced Direct Epoxidation of Propylene by Molecular Oxygen over CuO_x/TiO₂ Catalyst. Acta Physico-Chimica Sinica, 2025, 41(4): 100038-0. doi: 10.3866/PKU.WHXB202408015
16. [16]
  Hong RAO , Yang HU , Yicong MA , Chunxin LÜ , Wei ZHONG , Lihua DU . Synthesis and in vitro anticancer activity of phenanthroline-functionalized nitrogen heterocyclic carbene homo- and heterobimetallic silver/gold complexes. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2429-2437. doi: 10.11862/CJIC.20240275
17. [17]
  Li'na ZHONG , Jingling CHEN , Qinghua ZHAO . Synthesis of multi-responsive carbon quantum dots from green carbon sources for detection of iron ions and L-ascorbic acid. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 709-718. doi: 10.11862/CJIC.20240280
18. [18]
  Yiming Lu , Xiang Xie , Xiaoqing Qiu , Yang Liu , Xinyuan Cheng . The New Year’s Eve of the Aviation Brake Material Family. University Chemistry, 2024, 39(9): 203-207. doi: 10.12461/PKU.DXHX202403061
19. [19]
  Ke Qiu , Fengmei Wang , Mochou Liao , Kerun Zhu , Jiawei Chen , Wei Zhang , Yongyao Xia , Xiaoli Dong , Fei Wang . A Fumed SiO₂-based Composite Hydrogel Polymer Electrolyte for Near-Neutral Zinc-Air Batteries. Acta Physico-Chimica Sinica, 2024, 40(3): 2304036-0. doi: 10.3866/PKU.WHXB202304036
20. [20]
  Renxiao Liang , Zhe Zhong , Zhangling Jin , Lijuan Shi , Yixia Jia . A Palladium/Chiral Phosphoric Acid Relay Catalysis for the One-Pot Three-Step Synthesis of Chiral Tetrahydroquinoline. University Chemistry, 2024, 39(5): 209-217. doi: 10.3866/PKU.DXHX202311024

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(546)
HTML views(55)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142