Citation: ZHANG Yuqiong, ZHONG Yanlong, GAO Cuiyun, DONG Zhaorong, CHEN Na, WANG Meifang. Determination of five endogenous hormones in wheat by high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2013, 31(8): 800-803. doi: 10.3724/SP.J.1123.2013.01011 shu

Determination of five endogenous hormones in wheat by high performance liquid chromatography

1.
1. School of Life Sciences, Anhui Agricultural University, Hefei 230036, China;

Corresponding author: ZHANG Yuqiong, DONG Zhaorong,
Received Date: 5 January 2013
Available Online: 30 January 2013
Fund Project: 农业部科技项目国家公益性行业(农业)科研专项(201103001,201203032,201203096) (农业)科研专项(201103001,201203032,201203096)安徽省自然科学基金面上项目(1208085mc48). (1208085mc48)

A high performance liquid chromatographic (HPLC) method was developed to determine the five endogenous hormones including indole-3-acetic acid (IAA), abscisic acid (ABA), gibberellic acid (GA₃), zeatin (ZT) and salicylic acid (SA) in wheat. The separation conditions were optimized, and methanol was chosen as the extraction solvent. Then the extract was extracted by petroleum ether and ethyl acetate, and purified with the Sep-Pak C18 column. The chromatographic conditions were as follows: Eclipse XDB-C₁₈ reversed phase column (250 mm×4.6 mm, 5 μm), the flow rate of 1 mL/min, the injection volume of 10 μL, and the detection wavelength of 240 nm were used for the separation of SA from 14.5 min to 18 min, while the detection at 254 nm used for the separation of the others. Methanol (A) and acetic acid aqueous solution (pH 3.6) (B) were used as the mobile phases with the linear gradient set as follows: 0-7 min 20%A, 7-10 min 20%A-28%A, 10-17 min 28%A, 17-19 min 28%A-40%A, 19-35 min 40%A. The results showed that: the hormones were separated well with the recoveries of 96.9%-98%, and the RSDs were in the range of 1.54% to 2.29%. It is a reliable method for rapid, accurate separation and determination of the endogenous hormones in wheat.
- high performance liquid chromatography (HPLC),
- endogenous hormones,
- wheat
1. [1]
  [1] Maggio A, Barbieri G, Raimondi G, et al. J Plant Growth Regul, 2010, 29: 63
2. [2]
  [2] Liu H T, Li Y F, Luan T G, et al. Chromatographia, 2007, 66: 515
3. [3]
  [3] Zhang S L, Li Z Q. Journal of Wheat Research (张酸亮, 李赈群. 小麦研究), 1996, 17(3): 33
4. [4]
  [4] Zhao C J, Kang S J, Wang J H, et al. Acta Agriculturae Boreali-Sinica (赵春江, 康书江, 王纪华, 等. 华北农学报), 2000, 15(3): 51
5. [5]
  [5] He J L, Liu H X. Journal of Tropical and Subtropical Botany (贺继临, 刘鸿先. 热带亚热带植物学报), 1998, 6(4): 341
6. [6]
  [6] Liu C J, Liu Y Y, Li S Q, et al. Food Research and Development (刘长江, 刘旸旸, 李书倩, 等. 食品研究与开发), 2012, 33(2): 162
7. [7]
  [7] Tang Y, Wang L, Ma C, et al. Journal of Life Sciences, 2011, 5: 139
8. [8]
  [8] Yang T X, Wei A Z, Zheng Y, et al. Chinese Journal of Analytical Chemistry (杨途熙, 魏安智, 郑元, 等. 分析化学), 2007, 35: 1359
9. [9]
  [9] Fu J H, Chu J F, Wang J D, et al. Chinese Journal of Analytical Chemistry (符继红, 褚金芳, 王吉德, 等. 分析化学), 2009, 37(9): 1324
10. [10]
  [10] Yan S J, Guo L W, Wu C E, et al. Chinese Journal of Analytical Chemistry (闫师杰, 郭李维, 吴彩娥, 等. 分析化学), 2010, 38(6): 843
11. [11]
  [11] Chen B L, Zheng C X, Sheng J D, et al. Journal of Xinjiang Agricultural University (陈波浪, 郑春霞, 盛建东, 等. 新疆农业大学学报), 2006, 29(1): 28
12. [12]
  [12] Guo L J, Tan X Q, Lu P, et al. China Pharmacist (郭良君, 谭兴起, 陆萍, 等. 中国药师), 2009, 12(12): 1779
13. [13]
  [13] Zhang Z, Zhang Q, Wang Z H, et al. Chinese Journal of Chromatography (张政, 张强, 王转花, 等. 色谱), 1994, 12(2): 140
14. [14]
  [14] Raskin I. Plant Physiol, 1992, 99: 799
15. [15]
  [15] Wang S L, Wang P, Wang C Y. Journal of Analytical Science (王水良, 王平, 王趁义. 分析科学学报), 2010, 5(26): 547
16. [16]
  [16] Wang R Z, Xiao L T, Lin W H, et al. Chinese Journal of Chromatography (王若仲, 萧浪涛, 蔺万煌, 等. 色谱), 2002, 20(2): 148
17. [17]
  [17] Bu J J, Hu Y L, Wei C B, et al. Northern Horticulture (步佳佳, 胡玉林, 魏长斌, 等. 北方园艺), 2012(8): 30
1. [1]
  Jingming Li , Bowen Ding , Nan Li , Nurgul . Application of Comparative Teaching Method in Experimental Project Design of Instrumental Analysis Course: A Case Study in Chromatography Experiment Teaching. University Chemistry, 2024, 39(8): 263-269. doi: 10.3866/PKU.DXHX202312078
2. [2]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
3. [3]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
4. [4]
  Fan Wu , Wenchang Tian , Jin Liu , Qiuting Zhang , YanHui Zhong , Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031
5. [5]
  Hui Shi , Shuangyan Huan , Yuzhi Wang . Ideological and Political Design of Potassium Permanganate Oxidation-Reduction Titration Experiment. University Chemistry, 2024, 39(2): 175-180. doi: 10.3866/PKU.DXHX202308042
6. [6]
  Zhenlin Zhou , Siyuan Chen , Yi Liu , Chengguo Hu , Faqiong Zhao . A New Program of Voltammetry Experiment Teaching Based on Laser-Scribed Graphene Electrode. University Chemistry, 2024, 39(2): 358-370. doi: 10.3866/PKU.DXHX202308049
7. [7]
  Feng Liang , Desheng Li , Yuting Jiang , Jiaxin Dong , Dongcheng Liu , Xingcan Shen . Method Exploration and Instrument Innovation for the Experiment of Colloid ζ Potential Measurement by Electrophoresis. University Chemistry, 2024, 39(5): 345-353. doi: 10.3866/PKU.DXHX202312009
8. [8]
  Wei Peng , Baoying Wen , Huamin Li , Yiru Wang , Jianfeng Li . Exploration and Practice on Raman Scattering Spectroscopy Experimental Teaching. University Chemistry, 2024, 39(8): 230-240. doi: 10.3866/PKU.DXHX202312062
9. [9]
  Yujia Luo , Yunpeng Qi , Huiping Xing , Yuhu Li . The Use of Viscosity Method for Predicting the Life Expectancy of Xuan Paper-based Heritage Objects. University Chemistry, 2024, 39(8): 290-294. doi: 10.3866/PKU.DXHX202401037
10. [10]
  Tiejun Su . The Construction and Application of the Calculation Formula for Endpoint Error in Precipitation Titration: A Case Study of the Mohr Method. University Chemistry, 2024, 39(11): 384-387. doi: 10.12461/PKU.DXHX202402039
11. [11]
  Jiahao Lu , Xin Ming , Yingjun Liu , Yuanyuan Hao , Peijuan Zhang , Songhan Shi , Yi Mao , Yue Yu , Shengying Cai , Zhen Xu , Chao Gao . 基于稳态电热法的石墨烯膜导热系数的精确可靠测量. Acta Physico-Chimica Sinica, 2025, 41(5): 100045-. doi: 10.1016/j.actphy.2025.100045
12. [12]
  Bingliang Li , Yuying Han , Dianyang Li , Dandan Liu , Wenbin Shang . One-Step Synthesis of Benorilate Guided by Green Chemistry Principles and in vivo Dynamic Evaluation. University Chemistry, 2024, 39(6): 342-349. doi: 10.3866/PKU.DXHX202311070
13. [13]
  Mengyao Shi , Kangle Su , Qingming Lu , Bin Zhang , Xiaowen Xu . Determination of Potassium Content in Tobacco Stem Ash by Flame Atomic Absorption Spectroscopy. University Chemistry, 2024, 39(10): 255-260. doi: 10.12461/PKU.DXHX202404105
14. [14]
  Zhuo Wang , Xue Bai , Kexin Zhang , Hongzhi Wang , Jiabao Dong , Yuan Gao , Bin Zhao . MOF模板法合成氮掺杂碳材料用于增强电化学钠离子储存和去除. Acta Physico-Chimica Sinica, 2025, 41(3): 2405002-. doi: 10.3866/PKU.WHXB202405002
15. [15]
  Jiaojiao Yu , Bo Sun , Na Li , Cong Wen , Wei Li . Improvement of Classical Organic Experiment Based on the “Reverse-Step Optimization Method”: Taking Synthesis of Ethyl Acetate as an Example. University Chemistry, 2025, 40(3): 333-341. doi: 10.12461/PKU.DXHX202405177
16. [16]
  Ruiqing LIU , Wenxiu LIU , Kun XIE , Yiran LIU , Hui CHENG , Xiaoyu WANG , Chenxu TIAN , Xiujing LIN , Xiaomiao FENG . Three-dimensional porous titanium nitride as a highly efficient sulfur host. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 867-876. doi: 10.11862/CJIC.20230441
17. [17]
  Wenxiu Yang , Jinfeng Zhang , Quanlong Xu , Yun Yang , Lijie Zhang . Bimetallic AuCu Alloy Decorated Covalent Organic Frameworks for Efficient Photocatalytic Hydrogen Production. Acta Physico-Chimica Sinica, 2024, 40(10): 2312014-. doi: 10.3866/PKU.WHXB202312014
18. [18]
  Jie Li , Huida Qian , Deyang Pan , Wenjing Wang , Daliang Zhu , Zhongxue Fang . Efficient Synthesis of Anethaldehyde Induced by Visible Light. University Chemistry, 2024, 39(4): 343-350. doi: 10.3866/PKU.DXHX202310076
19. [19]
  Haoxiang Zhang , Zhihan Zhao , Yongchen Jin , Zhiqiang Niu , Jinlei Tian . Synthesis of an Efficient Absorbent Gel: A Recommended Comprehensive Chemistry Experiment. University Chemistry, 2024, 39(11): 251-258. doi: 10.12461/PKU.DXHX202401084
20. [20]
  Ran Yu , Chen Hu , Ruili Guo , Ruonan Liu , Lixing Xia , Cenyu Yang , Jianglan Shui . 杂多酸H₃PW₁₂O₄₀高效催化MgH₂储氢. Acta Physico-Chimica Sinica, 2025, 41(1): 2308032-. doi: 10.3866/PKU.WHXB202308032

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(226)
HTML views(19)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142