Citation: WANG Guojuan, ZHANG Kai, HE Xiwen, ZHANG Yukui. Identification of methylation at lysine 27 and 36 sites in histone H3[J]. Chinese Journal of Chromatography, ;2013, 31(6): 514-517. doi: 10.3724/SP.J.1123.2013.01008 shu

Identification of methylation at lysine 27 and 36 sites in histone H3

WANG Guojuan ^1,2 ,
ZHANG Kai ^1,2,, ,
HE Xiwen ² ,
ZHANG Yukui ^1,2,3

1.
1. State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, China;

Corresponding author: ZHANG Kai,
Received Date: 5 January 2013
Available Online: 11 March 2013
Fund Project: 国家重点基础研究发展计划("973"项目)(2012cb910601) ("973"项目)(2012cb910601)国家自然科学基金项目(21275077). (21275077)

we combined high performance liquid chromatography and tandem mass spectrometry (HPLC-MS/MS) with bioinformatics tools to analyze the isobaric modified peptides which were methylated and dimethylated at either lysine (K) 27 or/and K36 from histone H3. They were identified and dissected through alignment of every fragment ion, and the two modified sites were further analyzed according to their relative intensities of MS/MS spectra.
- liquid chromatography (LC),
- mass spectrometry (MS),
- histone,
- post-translational modifications (PTMs),
- quantitative proteomics
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