Citation: ZHOU Hongbin, XIONG Zhiyu, YU Yang, WAN Rong, LI Ping, SHEN Bo. Determination of alditols in wine by gas chromatography-mass spectrometry after acetate derivatization[J]. Chinese Journal of Chromatography, ;2013, 31(8): 786-790. doi: 10.3724/SP.J.1123.2013.01005 shu

Determination of alditols in wine by gas chromatography-mass spectrometry after acetate derivatization

ZHOU Hongbin ^1,, ,
XIONG Zhiyu ² ,
YU Yang ¹ ,
WAN Rong ¹ ,
LI Ping ¹ ,
SHEN Bo ¹

1.
1. State Key Laboratory of Food Additive and Condiment Testing, Zhenjiang 212008, China;

Corresponding author: ZHOU Hongbin,
Received Date: 5 January 2013
Available Online: 26 January 2013
Fund Project: 国家质量监督检验检疫总局公益性科研项目(2012424012-3). (2012424012-3)

The acetate derivatization of alditols for determining alditol level in wine by gas chromatography (GC)-mass spectrometry (MS) has been developed. The wine sample was mixed with pyridine and centrifuged at 5000 r/min at the temperature of 4℃ for 10 min. After filtration with organic phase membrane, the supernatant was derivatized with acetic anhydride, and then dehydrated with anhydrous sodium sulfate. The GC separation was performed on a DB-5MS capillary column. The alditols were determined by MS in selected ion monitoring (SIM) mode and quantified by external standard method. The calibration curves showed good linearities in the range of 0.019-1.25 mg/L except for lactitol (0.039-2.50 mg/L) with the correlation coefficients greater than 0.99. The limits of quantification (S/N=10) of erythritol, xylitol, D-mannitol, sorbitol, galactitol and lactitol were 0.17, 0.29, 0.43, 0.46, 0.47 and 2.88 mg/L respectively. The limits of detection (S/N=3) were 0.05, 0.08, 0.13, 0.14, 0.14 and 1.38 mg/L respectively. The recoveries of alditols spiked in the wine at two levels of 40 mg/L and 80 mg/L were ranged from 80.15% to 108.75% with the relative standard deviations (RSDs) of 2.16%-6.97%. The sensitivity, accuracy and precision of the method can meet the technical standard. The method can be applied to the rapid determination of alditols in wine.
- derivatization,
- gas chromatography-mass spectrometry (GC-MS),
- alditol,
- wine
1. [1]
  [1] Zhang H N, Yuan W, Qi X C, et al. Liquor Making (张会宁, 苑伟, 祁新春, 等. 酿酒), 2012, 39(5): 47
2. [2]
  [2] GB 2760-2011
3. [3]
  [3] Yu C, Yan Z, Cai L P, et al. Science and Technology of Food Industry (于趁, 闫正, 蔡立鹏, 等. 食品工业科技), 2010(3): 362
4. [4]
  [4] Tian Q, Fang C L, Xiu X H. China Food Additives (田强, 方春雷, 修秀红. 中国食品添加剂), 2009(4): 164
5. [5]
  [5] Ge C Y, Zhang J L, Chen J H. Chinese Journal of Chromatography (葛驰宇, 张君丽, 陈建华. 色谱), 2012, 30(8): 843
6. [6]
  [6] Zhao S, Yao J P, Ding X J, et al. Food Science (赵珊, 姚金苹, 丁晓静, 等. 食品科学), 2009, 30(2): 190
7. [7]
  [7] Zhang Y H, Jiang J G, Han X J, et al. China J Pharm Anal (张轶华, 姜建国, 韩学静, 等. 药物分析杂志), 2011, 31(7): 1341
8. [8]
  [8] Zheng Y J, Li Y L, Zhang X G, et al. Food and Fermentation Industries (郑彦婕, 黎永乐, 张协光, 等. 食品与发酵工业), 2010, 36(7): 154
9. [9]
  [9] Ma S M, Wang M T, Han D C, et al. China Food Additives (马书民, 王明泰, 韩大川, 等. 中国食品添加剂), 2011(5): 206
10. [10]
  [10] Zhou H H, Ma R L, Suo J. Journal of Instrumental Analysis (周红华, 马仁玲, 索瑾. 分析测试学报), 2003, 22(5): 110
11. [11]
  [11] Esteban N V, Liberato D J, Sidbury J B, et al. Anal Chem, 1987, 59: 1674
1. [1]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
2. [2]
  Yiling Wu , Peiyao Jin , Shenyue Tian , Ji Zhang . The Star of Sugar Substitutes: An Interview of Erythritol. University Chemistry, 2024, 39(9): 22-27. doi: 10.12461/PKU.DXHX202404034
3. [3]
  Zhuoming Liang , Ming Chen , Zhiwen Zheng , Kai Chen . Multidimensional Studies on Ketone-Enol Tautomerism of 1,3-Diketones By ¹H NMR. University Chemistry, 2024, 39(7): 361-367. doi: 10.3866/PKU.DXHX202311029
4. [4]
  Xiaowu Zhang , Pai Liu , Qishen Huang , Shufeng Pang , Zhiming Gao , Yunhong Zhang . Acid-Base Dissociation Equilibrium in Multiphase System: Effect of Gas. University Chemistry, 2024, 39(4): 387-394. doi: 10.3866/PKU.DXHX202310021
5. [5]
  Weina Wang , Fengyi Liu , Wenliang Wang . “Extracting Commonality, Delving into Typicals, Deriving Individuality”: Constructing a Knowledge Graph of Crystal Structures. University Chemistry, 2024, 39(3): 36-42. doi: 10.3866/PKU.DXHX202308029
6. [6]
  Jianfeng Yan , Yating Xiao , Xin Zuo , Caixia Lin , Yaofeng Yuan . Comprehensive Chemistry Experimental Design of Ferrocenylphenyl Derivatives. University Chemistry, 2024, 39(4): 329-337. doi: 10.3866/PKU.DXHX202310005
7. [7]
  Wen YANG , Didi WANG , Ziyi HUANG , Yaping ZHOU , Yanyan FENG . La promoted hydrotalcite derived Ni-based catalysts: In situ preparation and CO₂ methanation performance. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 561-570. doi: 10.11862/CJIC.20230276
8. [8]
  Ping Ye , Lingshuang Qin , Mengyao He , Fangfang Wu , Zengye Chen , Mingxing Liang , Libo Deng . 荷叶衍生多孔碳的零电荷电位调节实现废水中电化学捕集镉离子. Acta Physico-Chimica Sinica, 2025, 41(3): 2311032-. doi: 10.3866/PKU.WHXB202311032
9. [9]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
10. [10]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403
11. [11]
  Min LI , Xianfeng MENG . Preparation and microwave absorption properties of ZIF-67 derived Co@C/MoS₂ nanocomposites. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1932-1942. doi: 10.11862/CJIC.20240065
12. [12]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-. doi: 10.3866/PKU.WHXB202309036
13. [13]
  Yuhang Jiang , Weijie Liu , Jiaqi Cai , Jiayue Chen , Yanping Ren , Pingping Wu , Liulin Yang . A Journey into the Science and Art of Sugar: “Dispersion of Light and Optical Rotation of Matter” Science Popularization Experiment. University Chemistry, 2024, 39(9): 288-294. doi: 10.12461/PKU.DXHX202401054
14. [14]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . 液相法制备硫化物固体电解质及其在全固态锂电池中的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2309019-. doi: 10.3866/PKU.WHXB202309019
15. [15]
  Yonghui ZHOU , Rujun HUANG , Dongchao YAO , Aiwei ZHANG , Yuhang SUN , Zhujun CHEN , Baisong ZHU , Youxuan ZHENG . Synthesis and photoelectric properties of fluorescence materials with electron donor-acceptor structures based on quinoxaline and pyridinopyrazine, carbazole, and diphenylamine derivatives. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 701-712. doi: 10.11862/CJIC.20230373
16. [16]
  Shengbiao Zheng , Liang Li , Nini Zhang , Ruimin Bao , Ruizhang Hu , Jing Tang . Metal-Organic Framework-Derived Materials Modified Electrode for Electrochemical Sensing of Tert-Butylhydroquinone: A Recommended Comprehensive Chemistry Experiment for Translating Research Results. University Chemistry, 2024, 39(7): 345-353. doi: 10.3866/PKU.DXHX202310096
17. [17]
  Lina Guo , Ruizhe Li , Chuang Sun , Xiaoli Luo , Yiqiu Shi , Hong Yuan , Shuxin Ouyang , Tierui Zhang . 层状双金属氢氧化物的层间阴离子对衍生的Ni-Al₂O₃催化剂光热催化CO₂甲烷化反应的影响. Acta Physico-Chimica Sinica, 2025, 41(1): 2309002-. doi: 10.3866/PKU.WHXB202309002
18. [18]
  Yikai Wang , Xiaolin Jiang , Haoming Song , Nan Wei , Yifan Wang , Xinjun Xu , Cuihong Li , Hao Lu , Yahui Liu , Zhishan Bo . 氰基修饰的苝二酰亚胺衍生物作为膜厚不敏感型阴极界面材料用于高效有机太阳能电池. Acta Physico-Chimica Sinica, 2025, 41(3): 2406007-. doi: 10.3866/PKU.WHXB202406007
19. [19]
  Tianlong Zhang , Chenjia Song , Wenmin Zhao , Hongsheng Tang , Yan Li , Hua Li . Dream as a Horse, Poem “Liquor” while the Age. University Chemistry, 2024, 39(9): 48-54. doi: 10.12461/PKU.DXHX202403076
20. [20]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(266)
HTML views(13)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142