Citation: FENG Xiaoyan, ZHANG Jin, LV Meiling, GAO Mingxia, ZHANG Xiangmin. Detection of the major milk allergenα-casein in food with liquid chromatography-tandem mass spectrometry in multiple reaction monitoring mode[J]. Chinese Journal of Chromatography, ;2013, 31(6): 510-513. doi: 10.3724/SP.J.1123.2013.01003 shu

Detection of the major milk allergenα-casein in food with liquid chromatography-tandem mass spectrometry in multiple reaction monitoring mode

1.
1. Department of Chemistry, Fudan University, Shanghai 200433, China;

Corresponding author: ZHANG Xiangmin,
Received Date: 5 January 2013
Available Online: 2 February 2013
Fund Project: 科技部基础研究计划项目(2012CB910604) (2012CB910604)国家自然科学基金项目(21275034,21027002,21175026). (21275034,21027002,21175026)

A new method of detecting milk allergen protein in food was established. Using multiple reaction monitoring (MRM) mode in high performance liquid chromatography-mass spectrometry (HPLC-MS) to detect milk allergen protein α-casein is successful and convenient. All transitions gave a linear response in the chosen concentration range of the milk allergen protein (between 0.5 and 250 mg/L total soluble protein, 5 data points), and the detection limit is 0.5 mg/L of α-casein. Food samples from markets were tested. The results showed that MRM method could be used to detect the milk allergen in food products.
- multiple reaction monitoring (MRM),
- liquid chromatography-mass spectrometry(LC-MS),
- milk allergen,
- α-casein,
- food
1. [1]
  [1] Picariello G, Mamone G, Addeo F, et al. J Chromatogr A, 2011, 1218: 7386
2. [2]
  [2] Stevenson S E, Houston N L, Thelen J J. Regulatory Toxicology and Pharmacology, 2010, 58: S36
3. [3]
  [3] Guo L, Huo G C, Zhang Y H. Food Science and Technology (郭鸰, 霍贵成, 张英华. 食品科技), 2007(4): 262
4. [4]
  [4] Zhang X, Wu D X, Liu G H, et al. Journal of Food Safety and Quality (张霞, 吴冬雪, 刘国红, 等. 食品安全质量检测学报), 2012, 3(4): 290
5. [5]
  [5] Zhang Z J, Mao L T, Xiang J J, et al. Food Science and Technology (张在军, 毛露甜, 向军俭, 等. 食品科技), 2005(4): 69
6. [6]
  [6] Lange V, Picotti P, Domon B, et al. Molecular Systems Biology, 2008, 4: 222. DOI: 10.1038/msb.2008.61
7. [7]
  [7] Picotti P, Rinner O, Stallmach R, et al. Nature Methods, 2010, 7: 43
8. [8]
  [8] Zhao Y, Jia W, Wang J F, et al. Anal Chem, 2011, 83: 8802
9. [9]
  [9] Whiteaker J R, Zhang H, Zhao L, et al. J Proteome Res, 2007, 6: 3962
10. [10]
  [10] Monaci L, Losito I, Palmisano F, et al. J Chromatogr A, 2010, 1217: 4300
11. [11]
  [11] van Hengel A J. Anal Bioanal Chem, 2007, 389: 111
12. [12]
  [12] Monaci L, Tregoat V, van Hengel A J, et al. Eur Food Res Technol, 2006, 223: 149
13. [13]
  [13] Mao L T, Xiang J J, Zhang Z J. Food Science (毛露甜, 向军俭, 张在军. 食品科学), 2007, 28(5): 305
14. [14]
  [14] Heick J, Fischer M, Popping B. J Chromatogr A, 2011, 1218: 938
15. [15]
  [15] Ansari P, Stoppacher N, Rudolf J, et al. Anal Bioanal Chem, 2011, 399: 1105
1. [1]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
2. [2]
  Simin Fang , Hong Wu , Sizhe Sheng , Lingling Li , Yuxi Wang , Hongchun Li , Jun Jiang . The Food Kingdom Lecture Series: The Science behind Color. University Chemistry, 2024, 39(9): 177-182. doi: 10.12461/PKU.DXHX202402012
3. [3]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
4. [4]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . 液相法制备硫化物固体电解质及其在全固态锂电池中的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2309019-. doi: 10.3866/PKU.WHXB202309019
5. [5]
  Lingbang Qiu , Jiangmin Jiang , Libo Wang , Lang Bai , Fei Zhou , Gaoyu Zhou , Quanchao Zhuang , Yanhua Cui . 原位电化学阻抗谱监测长寿命热电池Nb₁₂WO₃₃正极材料的高温双放电机制. Acta Physico-Chimica Sinica, 2025, 41(5): 100040-. doi: 10.1016/j.actphy.2024.100040
6. [6]
  Tianlong Zhang , Rongling Zhang , Hongsheng Tang , Yan Li , Hua Li . Online Monitoring and Mechanistic Analysis of 3,5-diamino-1,2,4-triazole (DAT) Synthesis via Raman Spectroscopy: A Recommendation for a Comprehensive Instrumental Analysis Experiment. University Chemistry, 2024, 39(6): 303-311. doi: 10.3866/PKU.DXHX202312006
7. [7]
  Feiya Cao , Qixin Wang , Pu Li , Zhirong Xing , Ziyu Song , Heng Zhang , Zhibin Zhou , Wenfang Feng . Magnesium-Ion Conducting Electrolyte Based on Grignard Reaction: Synthesis and Properties. University Chemistry, 2024, 39(3): 359-368. doi: 10.3866/PKU.DXHX202308094
8. [8]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-. doi: 10.3866/PKU.WHXB202309036
9. [9]
  Yongjie ZHANG , Bintong HUANG , Yueming ZHAI . Research progress of formation mechanism and characterization techniques of protein corona on the surface of nanoparticles. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2318-2334. doi: 10.11862/CJIC.20240247
10. [10]
  Zeyu XU , Anlei DANG , Bihua DENG , Xiaoxin ZUO , Yu LU , Ping YANG , Wenzhu YIN . Evaluation of the efficacy of graphene oxide quantum dots as an ovalbumin delivery platform and adjuvant for immune enhancement. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1065-1078. doi: 10.11862/CJIC.20240099
11. [11]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
12. [12]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
13. [13]
  Peng GENG , Guangcan XIANG , Wen ZHANG , Haichuang LAN , Shuzhang XIAO . Hollow copper sulfide loaded protoporphyrin for photothermal-sonodynamic therapy of cancer cells. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1903-1910. doi: 10.11862/CJIC.20240155
14. [14]
  Min Gu , Huiwen Xiong , Liling Liu , Jilie Kong , Xueen Fang . Rapid Quantitative Detection of Procalcitonin by Microfluidics: An Instrumental Analytical Chemistry Experiment. University Chemistry, 2024, 39(4): 87-93. doi: 10.3866/PKU.DXHX202310120
15. [15]
  Xiyuan Su , Zhenlin Hu , Ye Fan , Xianyuan Liu , Xianyong Lu . Change as You Want: Multi-Responsive Superhydrophobic Intelligent Actuation Material. University Chemistry, 2024, 39(5): 228-237. doi: 10.3866/PKU.DXHX202311059
16. [16]
  Zongpei Zhang , Yanyang Li , Yanan Si , Kai Li , Shuangquan Zang . Developing a Chemistry Experiment Center Employing a Multifaceted Approach to Serve High-Quality Laboratory Education. University Chemistry, 2024, 39(7): 13-19. doi: 10.12461/PKU.DXHX202404041
17. [17]
  Ruilin Han , Xiaoqi Yan . Comparison of Multiple Function Methods for Fitting Surface Tension and Concentration Curves. University Chemistry, 2024, 39(7): 381-385. doi: 10.3866/PKU.DXHX202311023
18. [18]
  Xingyuan Lu , Yutao Yao , Junjing Gu , Peifeng Su . Energy Decomposition Analysis and Its Application in the Many-Body Effect of Water Clusters. University Chemistry, 2025, 40(3): 100-107. doi: 10.12461/PKU.DXHX202405074
19. [19]
  Yutong Dong , Huiling Xu , Yucheng Zhao , Zexin Zhang , Ying Wang . The Hidden World of Surface Tension and Droplets. University Chemistry, 2024, 39(6): 357-365. doi: 10.3866/PKU.DXHX202312022
20. [20]
  Gaoyan Chen , Chaoyue Wang , Juanjuan Gao , Junke Wang , Yingxiao Zong , Kin Shing Chan . Heart to Heart: Exploring Cardiac CT. University Chemistry, 2024, 39(9): 146-150. doi: 10.12461/PKU.DXHX202402011

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(328)
HTML views(12)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142