Citation: LIN Qin. Simultaneous determination of three β-lactamase inhibitor residues in dairy products by high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2013, 31(5): 441-446. doi: 10.3724/SP.J.1123.2012.12049 shu

Simultaneous determination of three β-lactamase inhibitor residues in dairy products by high performance liquid chromatography

LIN Qin ^,

1.
Fujian Inspection and Research Institute for Product Quality, Fuzhou 350002, China

Corresponding author: LIN Qin,
Received Date: 27 December 2012
Available Online: 30 January 2013
Fund Project: 国家质量监督检验检疫总局科技计划项目(2010QK021). (2010QK021)

An improved method has been developed for the determination of clavulanate potassium, tazobactam and sulbactam in dairy products. High performance liquid chromatography with photodiode array detector (HPLC-PAD) was used in this method. The separation was performed on a CAPCELL PAK MG-C₁₈ column (150 mm×4.6 mm, 5 μm). The mobile phase was 0.03% (volume fraction unless otherwise specified) phosphate-acetonitrile. This method offered effective sample preparation procedures including dissolution with water, protein isolation with acetone, clean-up and enrichment by solid-phase extraction (SPE) with PWAX cartridges (60 mg/3 mL) under weak acidic condition and further elution with 0.05% ammonia-methanol solution. The analytical method was well validated and good results were obtained with the RSDs of 2.2%-7.4% and the recoveries of 84.6%-101.7%. The detection limits of clavulanate potassium, tazobactam and sulbactam were 0.03 mg/L. All of the target compounds exhibited good linearities (r>0.999) over the mass concentration range of 0.05-5 mg/L. With the advantages of good purification effect, high sensitivity, good recovery and repeatability, the method is suitable for the detection of β-lactamase inhibitors in dairy products.
- high performance liquid chromatography (HPLC),
- β-lactamase inhibitors,
- clavulanate potassium,
- tazobactam,
- sulbactam,
- dairy products,
- residue
1. [1]
  [1] Ministry of Health of the People's Republic of China (PRC), Ministry of Industry and Information Technology of the PRC, Ministry of Public Security of the PRC, et al. Health Authority Issued[2009] No.21 (中华人民共和国卫生部,中华人民共和国工业和信息化部,中华人民共和国公安部,等. 卫监督发[2009] 21号). (2009-03-06). http://www.sfda.gov.cn/WS01/CL0056/38681.html
2. [2]
  [2] Ding T L, Zhang Y X. Shanghai Medical & Pharmaceutical Journal (丁天然, 张永信. 上海医药), 2011, 32(5): 222
3. [3]
  [3] Jayakar B, Kumudhavalli M, Margret Chandira R, et al. Int J PharmTech Res, 2010, 2(1): 906
4. [4]
  [4] Sahu S, Asati A, Toppo F, et al. Int J Pharm Pharm Sci, 2012, 4(4): 721
5. [5]
  [5] Dhandapani B, Thirumoorthy N, Harun Rasheed S, et al. Int J ChemTech Res, 2010, 2(1): 752
6. [6]
  [6] Hou Z Y, Wang L Q, Lei Y, et al. China Medicine and Pharmacy (侯志勇, 王立强, 雷严, 等. 中国医药科学), 2012, 2(17): 98
7. [7]
  [7] Cao G X, Zhong C Q, Chen F, et al. Chinese Pharmaceutical Affairs (曹广祥, 钟传青, 陈仿, 等. 中国药事), 2008, 22(12): 1090
8. [8]
  [8] Sun Q, Zhang L T, Wang Y L, et al. Chinese Journal of Hospital Pharmacy (孙倩, 张兰桐, 王永利, 等. 中国医院药学杂志), 2011, 31(2): 94
9. [9]
  [9] Jiao X M, Zhao X D, Wang Q Y. Chinese Journal of Pharmaceutical Analysis (矫筱蔓, 赵晓冬, 王全一. 药物分析杂志), 2011, 31(3): 504
10. [10]
  [10] Ying G H, Wang Y, Deng Y, et al. Chinese Journal of Pharmaceutical Analysis (应国红, 王玉, 邓颖, 等. 药物分析杂志), 2009, 29(11): 1797
11. [11]
  [11] Sun Y X, Zhao L M, He X J, et al. Chinese Pharmaceutical Journal (孙亚欣, 肇丽梅, 何晓静, 等. 中国药学杂志), 2008, 43(21): 1651
12. [12]
  [12] Liu Y W, Sun P H, Zhou Ying, et al. Chinese Journal of Clinical Pharmacology (刘玉旺, 孙培红, 周颖, 等. 中国临床药理学杂志), 2001, 17(5): 360
13. [13]
  [13] Zhuo H T, Cao X M, Cao W, et al. Chinese Journal of Clinical Pharmacology (卓海通, 曹晓梅, 曹文, 等. 中国临床药理学杂志), 2000, 16(2): 111
14. [14]
  [14] Lin Z W, Li Y, Song W J, et al. Journal of Southern Medical University (林祖文, 李源, 宋玮娟, 等. 南方医科大学学报), 2011, 31(6): 1069
15. [15]
  [15] Gu Y, Wang X J, Wang R, et al. Journal of the Fourth Military Medical University (顾宜, 王晓娟, 王荣, 等. 第四军医大学学报), 2006, 27(22): 2106
16. [16]
  [16] Zhang J, Chen X Y, Dai X J, et al. Chinese Journal of Pharmaceutical Analysis (张娟, 陈笑艳, 戴晓健, 等. 药物分析杂志), 2012, 32(1): 20
17. [17]
  [17] Ye X C, Fan G R, Zhu D Q. China Pharmacy (叶显撑, 范国荣, 祝德秋. 中国药房), 2012, 23(6): 531
18. [18]
  [18] Liu S S, Shan Y, Man C X, et al. Science and Technology of Food Industry (刘珊珊, 单艺, 满朝新, 等. 食品工业科技), 2012, 33(16): 64
19. [19]
  [19] Xue X, Wang J, Liu G L, et al. Journal of Instrumental Analysis (薛霞, 王骏, 刘桂亮, 等. 分析测试学报), 2012, 31(6): 700
20. [20]
  [20] Guo Q L, Wang H, Yang H M, et al. Journal of Chinese Mass Spectrometry Society (郭启雷, 王浩, 杨红梅, 等. 质谱学报), 2009, 30(Suppl): 162
1. [1]
  Fan Wu , Wenchang Tian , Jin Liu , Qiuting Zhang , YanHui Zhong , Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031
2. [2]
  Siming Bian , Sijie Luo , Junjie Ou . Application of van Deemter Equation in Instrumental Analysis Teaching: A New Type of Core-Shell Stationary Phase. University Chemistry, 2025, 40(3): 381-386. doi: 10.12461/PKU.DXHX202406087
3. [3]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
4. [4]
  Hong Zheng , Xin Peng , Chunwang Yi . The Tale of Caprolactam Cyclic Oligomers: The Ever-changing Life of “Princess Cyclo”. University Chemistry, 2024, 39(9): 40-47. doi: 10.12461/PKU.DXHX202403058
5. [5]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
6. [6]
  Jiahe LIU , Gan TANG , Kai CHEN , Mingda ZHANG . Effect of low-temperature electrolyte additives on low-temperature performance of lithium cobaltate batteries. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 719-728. doi: 10.11862/CJIC.20250023
7. [7]
  Shengjuan Huo , Xiaoyan Zhang , Xiangheng Li , Xiangning Li , Tianfang Chen , Yuting Shen . Unveiling the Marvels of Titanium: Popularizing Multifunctional Colored Titanium Product Films. University Chemistry, 2024, 39(5): 184-192. doi: 10.3866/PKU.DXHX202310127
8. [8]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . 液相法制备硫化物固体电解质及其在全固态锂电池中的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2309019-. doi: 10.3866/PKU.WHXB202309019
9. [9]
  Meijin Li , Xirong Fu , Xue Zheng , Yuhan Liu , Bao Li . The Marvel of NAD⁺: Nicotinamide Adenine Dinucleotide. University Chemistry, 2024, 39(9): 35-39. doi: 10.12461/PKU.DXHX202401027
10. [10]
  Ruiqing LIU , Wenxiu LIU , Kun XIE , Yiran LIU , Hui CHENG , Xiaoyu WANG , Chenxu TIAN , Xiujing LIN , Xiaomiao FENG . Three-dimensional porous titanium nitride as a highly efficient sulfur host. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 867-876. doi: 10.11862/CJIC.20230441
11. [11]
  Ran Yu , Chen Hu , Ruili Guo , Ruonan Liu , Lixing Xia , Cenyu Yang , Jianglan Shui . 杂多酸H₃PW₁₂O₄₀高效催化MgH₂储氢. Acta Physico-Chimica Sinica, 2025, 41(1): 2308032-. doi: 10.3866/PKU.WHXB202308032
12. [12]
  Peng XU , Shasha WANG , Nannan CHEN , Ao WANG , Dongmei YU . Preparation of three-layer magnetic composite Fe₃O₄@polyacrylic acid@ZiF-8 for efficient removal of malachite green in water. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 544-554. doi: 10.11862/CJIC.20230239
13. [13]
  Qilong Fang , Yiqi Li , Jiangyihui Sheng , Quan Yuan , Jie Tan . Magical Pesticide Residue Detection Test Strips: Aptamer-based Lateral Flow Test Strips for Organophosphorus Pesticide Detection. University Chemistry, 2024, 39(5): 80-89. doi: 10.3866/PKU.DXHX202310004
14. [14]
  Yutong Dong , Huiling Xu , Yucheng Zhao , Zexin Zhang , Ying Wang . The Hidden World of Surface Tension and Droplets. University Chemistry, 2024, 39(6): 357-365. doi: 10.3866/PKU.DXHX202312022
15. [15]
  Gaoyan Chen , Chaoyue Wang , Juanjuan Gao , Junke Wang , Yingxiao Zong , Kin Shing Chan . Heart to Heart: Exploring Cardiac CT. University Chemistry, 2024, 39(9): 146-150. doi: 10.12461/PKU.DXHX202402011
16. [16]
  Wenkai Chen , Yunjia Shen , Xiangmeng Kong , Yanli Zeng . Quantum Chemistry Calculation of Key Physical Quantity in Circularly Polarized Luminescence: Introducing an Exploratory Computational Chemistry Experiment. University Chemistry, 2025, 40(3): 83-91. doi: 10.12461/PKU.DXHX202405018
17. [17]
  Xiaowu Zhang , Pai Liu , Qishen Huang , Shufeng Pang , Zhiming Gao , Yunhong Zhang . Acid-Base Dissociation Equilibrium in Multiphase System: Effect of Gas. University Chemistry, 2024, 39(4): 387-394. doi: 10.3866/PKU.DXHX202310021
18. [18]
  Qingtang ZHANG , Xiaoyu WU , Zheng WANG , Xiaomei WANG . Performance of nano Li₂FeSiO₄/C cathode material co-doped by potassium and chlorine ions. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1689-1696. doi: 10.11862/CJIC.20240115
19. [19]
  Mengyao Shi , Kangle Su , Qingming Lu , Bin Zhang , Xiaowen Xu . Determination of Potassium Content in Tobacco Stem Ash by Flame Atomic Absorption Spectroscopy. University Chemistry, 2024, 39(10): 255-260. doi: 10.12461/PKU.DXHX202404105
20. [20]
  Shahua Huang , Xiaoming Guo , Lin Lin , Guangping Chang , Sheng Han , Zuxin Zhou . Application of “Integration of Industry and Education” in Engineering Chemistry: Improvement of the Pesticide Fipronil Production. University Chemistry, 2024, 39(3): 199-204. doi: 10.3866/PKU.DXHX202309064

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(164)
HTML views(0)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142