Citation: LIN Qin. Simultaneous determination of three β-lactamase inhibitor residues in dairy products by high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2013, 31(5): 441-446. doi: 10.3724/SP.J.1123.2012.12049 shu

Simultaneous determination of three β-lactamase inhibitor residues in dairy products by high performance liquid chromatography

LIN Qin ^,

1.
Fujian Inspection and Research Institute for Product Quality, Fuzhou 350002, China

Corresponding author: LIN Qin,
Received Date: 27 December 2012
Available Online: 30 January 2013
Fund Project: 国家质量监督检验检疫总局科技计划项目(2010QK021). (2010QK021)

An improved method has been developed for the determination of clavulanate potassium, tazobactam and sulbactam in dairy products. High performance liquid chromatography with photodiode array detector (HPLC-PAD) was used in this method. The separation was performed on a CAPCELL PAK MG-C₁₈ column (150 mm×4.6 mm, 5 μm). The mobile phase was 0.03% (volume fraction unless otherwise specified) phosphate-acetonitrile. This method offered effective sample preparation procedures including dissolution with water, protein isolation with acetone, clean-up and enrichment by solid-phase extraction (SPE) with PWAX cartridges (60 mg/3 mL) under weak acidic condition and further elution with 0.05% ammonia-methanol solution. The analytical method was well validated and good results were obtained with the RSDs of 2.2%-7.4% and the recoveries of 84.6%-101.7%. The detection limits of clavulanate potassium, tazobactam and sulbactam were 0.03 mg/L. All of the target compounds exhibited good linearities (r>0.999) over the mass concentration range of 0.05-5 mg/L. With the advantages of good purification effect, high sensitivity, good recovery and repeatability, the method is suitable for the detection of β-lactamase inhibitors in dairy products.
- high performance liquid chromatography (HPLC),
- β-lactamase inhibitors,
- clavulanate potassium,
- tazobactam,
- sulbactam,
- dairy products,
- residue
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