Citation: ZHAO Hui, WANG Yuhong, LIU Fang, WANG Yan, GU Xue, YAN Chao. Microfluidic evaporative light scattering detector coupled with capillary liquid chromatography and its application to Ginkgo biloba extract analysis[J]. Chinese Journal of Chromatography, ;2013, 31(8): 795-799. doi: 10.3724/SP.J.1123.2012.12043 shu

Microfluidic evaporative light scattering detector coupled with capillary liquid chromatography and its application to Ginkgo biloba extract analysis

1.
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China

Corresponding author: YAN Chao,
Received Date: 25 December 2012
Available Online: 2 February 2013
Fund Project: 国家自然科学基金项目(21175092,21105064) (21175092,21105064)国家重大科学仪器设备开发专项(2011YQ150072,2011YQ15007204,2011YQ15007207,2011YQ15007210) (2011YQ150072,2011YQ15007204,2011YQ15007207,2011YQ15007210)上海市自然科学基金项目(12ZR1413600) (12ZR1413600) 上海市经信委引进技术的吸收与创新计划项目(2010XI-007). (2010XI-007)

A novel separation system of microfluidic evaporative light scattering detector( μELSD) coupled with capillary liquid chromatography (cLC) was built and applied to the separation and detection of herbal medicine Ginkgo biloba extract and its disperse tablet formulation. Compared with the traditional HPLC, this μELSD-cLC system consumed much less sample and solvent. Some key parameters were optimized. It was found that the higher the evaporization temperature of the drift tube, the higher S/N could be achieved. The mobile phase A was 0.05% (v/v) trifluoroacetic acid (TFA), and the mobile phase B was methanol containing 0.05% (v/v) TFA. The optimized gradient conditions were as follows: 0-10 min, 5%B-25%B; 10-25 min, 25%B-38%B; 25-35 min, 38%B; 35-40 min, 38%B-42%B; 40-55 min, 42%B-50%B. The complex herbal medicine Ginkgo biloba extract and its disperse tablet formulation were successfully separated. Four main therapeutic components (bilobalide and ginkgolide A, B, C) were finally separated and determined, which were terpene lactones with pretty weak UV absorption. The RSDs of the detected terpene lactones from different manufacturers were all no more than 2.42%, which proved this platform’s good analysis repeatability. The results showed the applicability of the platform to the analysis of complex traditional Chinese medicines.
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沈阳化工大学材料科学与工程学院沈阳 110142