Citation: SHI Dongqin, WANG Rong, XIE Hua, TIAN Wei, JIA Zhengping, GUO Jiankui. Determination of K-ras gene mutation in colorectal cancer tissue by capillary electrophoresis[J]. Chinese Journal of Chromatography, ;2013, 31(6): 582-586. doi: 10.3724/SP.J.1123.2012.12023 shu

Determination of K-ras gene mutation in colorectal cancer tissue by capillary electrophoresis

SHI Dongqin ^1,2 ,
WANG Rong ^1,2,, ,
XIE Hua ¹ ,
TIAN Wei ² ,
JIA Zhengping ^1,, ,
GUO Jiankui ¹

1.
1. PLA Key Laboratory of the Plateau of the Environmental Damage Control, Base of Clinic Pharmacology, Lanzhou General Hospital, Lanzhou 730050, China;

Corresponding author: WANG Rong, JIA Zhengping,
Received Date: 12 December 2012
Available Online: 14 January 2013
Fund Project: 国家自然科学基金项目(20775089) (20775089)全军医药卫生"十一五"科研课题(06MB096). (06MB096)

The codons 12/13 of K-ras genomic DNA from 76 colorectal cancer tissues and normal tissues were amplified by PCR. The amplified 152 samples were purified, denatured, and then detected using capillary electrophoresis (CE)-laser induced fluorescence (LIF) with single-strand conformation polymorphism (SSCP). The abnormal samples were further confirmed by direct-sequencing. The 30 patients of the 76 colorectal cancer patients were found gene mutation, of which the results of base G→A point mutation were confirmed by the gene sequence. To detect K-ras gene mutation that has important role in clinical forecast, diagnosis, therapy and prognosis, the CE-LIF with SSCP was applied for detecting K-ras gene. It becomes a promising tool to analyse K-ras gene mutation in the clinical diagnosis and therapy of colorectal cancer tissue.
- capillary electrophoresis (CE),
- K-ras gene,
- single-strand conformation polymorphism,
- gene mutation,
- colorectal cancer tissue
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