Citation: WANG Ji-Pin, BAI Guang-Xin, LI Jin-Ran, WANG Ye-Bao, LENG Ji-Yan, QIN Jun-Jie. Solvent Extraction for Determination of Blueberry Anthocyanins[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(12): 1952-1953. doi: 10.3724/SP.J.1096.2012.20901
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A solvent extraction method was developed for the determination of blueberry anthocyanins. The blueberries samples were coarse extracted twice by acid ethanol. After purificated by the AB-8 macroporous resin, the blueberries extract was vacuum dried. The components of blueberries extract were identified by FeCl3 color reaction and neutral acetic acid lead reaction, and then determined by infrared spectrometry and UV-vis method. The total content of anthocyanins was calculated by pH differential method following by the equation: produce=absorbency×the diluted times of determination spectrophotometry from samples/the quality (g). The results show that the extract of blueberry is part of flavonoids, the extract content is 2.29 mg/g, and the color value is 36.
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Keywords:
- Solvent extraction
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