Citation: ZHOU Hai-Ying, ZHOU Rui, XIONG Bin, HE Yan. Preparation and Cytotoxicity of High-aspect-ratio Gold Nanorods at Single Cell Level[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(12): 1807-1815. doi: 10.3724/SP.J.1096.2012.20793 shu

Preparation and Cytotoxicity of High-aspect-ratio Gold Nanorods at Single Cell Level

1.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha 410082, China

Corresponding author: HE Yan,
Received Date: 26 July 2012
Available Online: 19 September 2012
Fund Project: 本文系国家自然科学基金项目(No.20975036)资助 (No.20975036)

We have synthesized high-aspect-ratio gold nanorods (GNR) by using a three-step seed-mediated growth method. The aspect ratio of the GNRs is approximately 14. The modification of the GNRs was achieved by replacing the CTAB molecules on the surface of gold nanorods with the 11-mercaptoundecanoic (MUDA) molecules. The cytotoxicity of the as-prepared GNRs and their effects on endocytosis, adhesion, proliferation, intracellular reactive oxygen species (ROS) level and cytoskeleton of the cells were studied. Interestingly, by using the 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyl-tetrazolium bromide (MTT) assay, the GNRs did not show a significant toxicity to HeLa cells. However, single cell viability assays showed that GNR uptake could influence the cell adhesion at the early stage, though the effect was not much, and the cell proliferation was promoted to some degree. Moreover, large amounts of GNR uptake will lead to increased intracellular ROS level and impaired the cell skeleton.
- High-aspect-ratio gold nanorods,
- Surface modification,
- Cytotoxicity
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沈阳化工大学材料科学与工程学院沈阳 110142