Citation: GUO Zhi-Ying, LIANG Yue-Qin, CUI Xiao-Lei, YU Shui. Determination of Depleted Uranium and ²³⁵U/²³⁸U of Tissues and Combined with DNA in Mouse by Inductively Coupled Plasma Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(12): 1865-1870. doi: 10.3724/SP.J.1096.2012.20627 shu

Determination of Depleted Uranium and ²³⁵U/²³⁸U of Tissues and Combined with DNA in Mouse by Inductively Coupled Plasma Mass Spectrometry

1.
Biomedical Analysis Center, Academy of Military Medical Sciences, Beijing 100850, China

Corresponding author: YU Shui,
Received Date: 18 June 2012
Available Online: 8 August 2012
Fund Project: 本文系国家科技部创新方法研究项目(No.2008IM021500) (No.2008IM021500)国家自然科学基金项目(No.81102072) (No.81102072)第47批博士后基金项目(No.20100471003)资助 (No.20100471003)

The BALB/c mice was exposed to deplete uranium (DU) solution (²³⁵U/²³⁸U=0.00431). Blood and the tissues of kidney, liver, spleen, cerebrum, femur, lung and testicle were collected in different time after the exposion. The uranium concentration and ²³⁵U/²³⁸U ratio in tissues were determined simultaneously by inductively coupled plasma mass spectrometry (ICP-MS), and to analyze the distribution and metabolism of DU in the body. The extracted and purified genomic DNA in kidney and liver was dialyzed to excluding the DU uncombined, and quantified by ultraviolet spectrophotometry. Then the DNA was diluted with special diluents, and the binding level of DU with genomic DNA in kidney and liver was detected by ICP-MS. Pt was confirmed as the internal standard to compensate matrix influence. The limits of detection in tissues were in the range of 0.0019 μg/kg to 0.0981 μg/kg. The precision (RSD) ranged from 0.9% to 2.1%, and the recovery was about 100%±10%. The results of ²³⁵U/²³⁸U isotopic ratio in the depleted uranium aerosols detected by ICP-MS were consistent with the results detected by α-spectrometer, and RSD=6%. The genomic DNA was diluted with neutral solution. The detection limit of uranium in DNA was 0.0016 μg/kg, and the recovery was 98.3%±7.3%. The analytical results showed that DU could be differentially accumulated in different tissues, and most of DU was accumulated in kidney and bone, and scarcely enter into the brain tissue. Especially, DU would be accumulated in bone for a long time. DU could combine with genomic DNA in kidney and liver, and the binding level of DU in kidney was relatively high and persistent. The uranium concentration in tissues had relationship to some extent with ²³⁵U/²³⁸U, and the ²³⁵U/²³⁸U isotopic ratio would be altered with uranium concentration. The uranium concentration and ²³⁵U/²³⁸U can be sensitive indicator to measure the pollution of uranium.
- Inductively coupled plasma mass spectrometry,
- Depleted uranium,
- Isotopic ratio of uranium,
- Deoxyribonucleic acid
1. [1]
  1 Shawky S. East Mediterr Health J., 2002, 8 (2-3): 432-439
2. [2]
  2 Briner W. Int J Environ Res Public Health, 2010, 7(1): 303-313
3. [3]
  3 YU Shui, ZOU Xiao-Ying, ZHONG Wei-Qiang, WANG Gong-Peng, GUO Li-Sheng. Chin. J. Radio. Med. Prot., 2001, 21(2): 90-92
4. [4]
  于水, 邹晓颖, 仲伟强, 王功鹏, 郭力生. 中华放射医学与防护杂志, 2001, 21(2): 90-92
5. [5]
  4 Fairlie I. Med. Confl. Surviv., 2009, 25(1): 41-64
6. [6]
  5 LIANG Zhi-Rong, CHEN Qi, LIU Xue-Jun. Journal of Nuclear Radiochemistry, 2007, 29(1): 57-59
7. [7]
  梁志荣, 陈琦, 刘学军. 核化学与放射化学, 2007, 29(1): 57-59
8. [8]
  6 LI Rong, AI Guo-Ping, XU Hui, REN Jiong, LOU Shu-Fen, CHENG Tian-Min, SU Yong-Ping, ZHENG Huai-En, JIANG Jian-Xin, HUANG Yue-Sheng. J. Fourth Mil Med Univ., 2004, 25(6): 503-506
9. [9]
  李蓉, 艾国平, 徐辉, 任泂, 楼淑芬, 程天民, 粟永萍, 郑怀恩, 蒋建新, 黄跃生. 第四军医大学学报, 2004, 25(6): 503-506
10. [10]
  7 ZENG Jing, YAN Lai-Lai, OUYANG Li, WANG Jing-Yu. Chinese J. Anal Chem., 2011, 39(8): 1176-1180
11. [11]
  曾静, 闫赖赖, 欧阳荔, 王京宇. 分析化学, 2011, 39(8): 1176-1180
12. [12]
  8 ZHANG Lin-Lin, XING Xiao-Ru, WU Guo-Ping, WEI Fu-Sheng. Spectroscopy and Spectral Analysis, 2009, 29(4): 1115-1118
13. [13]
  张霖琳, 邢小茹, 吴国平, 魏复盛. 光谱学与光谱分析, 2009, 29(4): 1115-1118
14. [14]
  9 Castro W, Hoogewerff J, Latkoczy C, Almirall J R. Forensic Sci. Int., 2010, 195(1): 17-27
15. [15]
  10 YANG Zhi-Hua, LU Ying, CAO Zhen-Shan, YU Shui, ZHU Mao-Xiang. J. Health Toxicol., 2002, 16(3): 146-149
16. [16]
  杨陟华, 陆颖, 曹珍山, 于水, 朱茂祥. 卫生毒理学杂志, 2002, 16(3): 146-149
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沈阳化工大学材料科学与工程学院沈阳 110142

Determination of Depleted Uranium and 235U/238U of Tissues and Combined with DNA in Mouse by Inductively Coupled Plasma Mass Spectrometry

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通讯作者: 陈斌, bchen63@163.com

Determination of Depleted Uranium and ²³⁵U/²³⁸U of Tissues and Combined with DNA in Mouse by Inductively Coupled Plasma Mass Spectrometry