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Citation: YU Rui-Xiang, YANG Rui-Yu, ZHANG Xin, FANG Xiao-Ming, DING Zhuo-Ping. Simultaneous Determination of Four Steradienes in Vegetable Oils by High Performance Liquid Chromatography[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(12): 1902-1906. doi: 10.3724/SP.J.1096.2012.20610 shu

Simultaneous Determination of Four Steradienes in Vegetable Oils by High Performance Liquid Chromatography

1.
¹ College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China;

Corresponding author: FANG Xiao-Ming, DING Zhuo-Ping,
Received Date: 14 June 2012
Available Online: 12 July 2012
Fund Project: 本文系国家质检总局科研项目(No.2012IK184) (No.2012IK184)上海市教育委员会重点学科建设项目(No.J50704)资助 (No.J50704)

A method for the simultaneous determination of four steradienes in vegetable oils by high performance liquid chromatography (HPLC) was established. The sample was dissolved in petroleum ether and passed through a silica gel column, and the analytes were eluted with petroleum ether. The chromatographic separation was carried out on a C30 column (250 mm×4.6 mm i.d, 5 μm) using UV detection at 235 nm. Acetonitrile/tert-butyl methyl ether (75:25, V/V) was served as mobile phase in isocratic elution. Results showed good linear ranges for 4 steradienes from 0.025 mg/L to 1.0 mg/L with correlation coefficients(r²)>0.999. The mean recoveries were in the range of 89.2%-109.8% at three spiked levels of 0.050, 0.10 and 0.50 mg/kg, and the relative standard deviations (RSDs) were less than 8%. The limits of detection (LODs) were of 0.010 mg/kg. The proposed method had been applied in real sample analysis.
- High performance liquid chromatography,
- Steradienes,
- Vegetable oils
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