Citation: ZHAO Yue-Liang, WANG Feng, ZHOU Hong-Jie, LI Wei-Ming, LI An, LIU Hai-Jing, HA Yi-Ming. Determination of 2-Dodecylcyclobutanone in Irradiated Beef by Direct Solvent Extraction and Gas Chromatography-Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(12): 1919-1923. doi: 10.3724/SP.J.1096.2012.20428 shu

Determination of 2-Dodecylcyclobutanone in Irradiated Beef by Direct Solvent Extraction and Gas Chromatography-Mass Spectrometry

1.
Institute of Agro-food Science & Technology Chinese Academy of Agricultural Sciences, Key Opening Laboratory of Agricultural Products Processing and Quality Control, Ministry of Agriculture, Beijing 100193, China

Corresponding author: HA Yi-Ming,
Received Date: 22 April 2012
Available Online: 13 August 2012
Fund Project: 本文系"十二五"国家科技支撑计划(Nos.2012BAK17B13,2012BAD29B03) (Nos.2012BAK17B13,2012BAD29B03)农业部公益性行业科研专项(No.201103007)资助项目 (No.201103007)

A new rapid analytical method based on direct solvent extraction (DSE)/gas chromatography-mass spectrometry (GC-MS) was developed to analyze 2-dodecyclobutanone (2-DCB) to detect γ-ray irradiated beef. A 5 g sample of ground beef patties was homogenized with 7 g of anhydrous sodium sulfate and blended with 150 mL acetonitrile using a blender for 2 min. The extract solvent was carefully transferred to a 500 mL round-bottom flask through filter paper. And the procedure repeated for two times. Then, the extraction solutions were evaporated to dryness using a rotary evaporator. The round-bottom flask was washed with hexane, and the solution was collected into a 50 mL volumetric flask. The 10 mL hexane aliquot was dried to 1 mL via nitrogen stream and then purified with a silica cartridge (1 g, 6 mL). The elution was concentrated to 1 mL with a nitrogen stream, and 1 μL was injected into the gas chromatography-mass spectrometer. The limit of detection and quantitation was 0.004 and 0.01 mg/kg, respectively. The procedure was evaluated with a recovery test in six samples spiked with 2-DCB at 0.01 and 0.5mg/kg of ground beef, resulting in 87.9%-91.6% recoveries with mostly less than 7% RSD. The operation time for sample preparation was about 60 to 90 min. The method was used to detect 2-DCB in ground beef irradiated at 5 targeted absorbed doses of 0.5, 1.0, 3.0, 5.0 and 7.0 kGy and the concentration of 2-DCB increased linearly with dose (y=0.0608x-0.0004, R²=0.9899).
- 2-Dodecyclobutanone,
- Irradiated beef,
- Direct solvent extraction,
- Gas chromatography-mass spectrometry
1. [1]
  1 Gadgil P G, Hachmeister K A, Smith J S, Kropf D H. J. Agric. Food Chem., 2002, 50(20): 5746-5750
2. [2]
  2 Obana H, FurutaM, Tanaka Y. J. Agric. Food Chem., 2005, 53(17): 6603-6608
3. [3]
  3 Obana H, Furuta M, Tanaka Y. J. Food Hyg. Soci. Japan, 2007, 48(6): 203-206
4. [4]
  4 Foodstuffs-Detection of Irradiated Foods Containing Fat-Gas Chromatography/Mass Spectrometric Analysis of 2-Alkylcyclobutanone. British and European Standard. BS EN 1785: 2003
5. [5]
  5 Tewfik I H, Ismail H M, Sumar S. Int. J. Food Sci. Nutr., 1999, 50(1): 51-56
6. [6]
  6 HAN Li, WANG Min, WANG Chuan-Xian, YANG Jie-Lin, YANG Zhen-Yu, GUO De-Hua. J. Instrum. Anal., 2009, 28(1): 76-79
7. [7]
  韩丽, 王敏, 王传现, 杨捷琳, 杨振宇, 郭德华. 分析测试学报, 2009, 28(1): 76-79
8. [8]
  7 Chen S, Morita Y, Saito K, Kameya H, Nakajima M, Todoriki S. J. Agric. Food Chem., 2011, 59(1): 78-84
9. [9]
  8 Gupta MN, Batra R, Tyagi R, Sharma A. Biotechnol. Prog., 1997, 13(3): 284-288
10. [10]
  9 ZHANG Hai-Wei, HA Yi-Ming, WANG Feng, LIN Ruo-Tai. Scientia Agricultura Sinica. 2009, 42(3): 989-995
11. [11]
  张海伟, 哈益明, 王锋, 林若泰. 中国农业科学, 2009, 42(3): 989-995
12. [12]
  10 Gadgil P, Hachmeister K A, Smith J S, Kropf D H. J. Agric. Food Chem., 2002, 50(20): 5746-5750
13. [13]
  11 Gadgil P, Smith J S, Hachmeister K A, Kropf D H. J. Agric. Food Chem., 2005, 53(6): 1890-1893
14. [14]
  12 Zanardi E, Battaglia A, Ghidini S, Conter M, Badiani A, Ianieri A. J. Agric. Food Chem., 2007, 55: 4264-4270
15. [15]
  13 Tewfik I. Int. J. Food Sci. Nut., 2008, 59(7-8): 590-598
16. [16]
  14 D'oca M C, Bartolotta A, Cammilleri M C, Giuffrida S A, Parlato A, Di Noto A M, Caracappa S. J. Radiate Phys. Chem., 2009, 78: 687-689
17. [17]
  15 Soncin S, Panseri S, Rusconi M, Mariani M, Chiesa L M, Biondi P A. Food Chem., 2012 , doi:10.1016/j.foodchem. 2012.02.089
1. [1]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
2. [2]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
3. [3]
  Zhuoyan Lv , Yangming Ding , Leilei Kang , Lin Li , Xiao Yan Liu , Aiqin Wang , Tao Zhang . Light-Enhanced Direct Epoxidation of Propylene by Molecular Oxygen over CuO_x/TiO₂ Catalyst. Acta Physico-Chimica Sinica, 2025, 41(4): 100038-. doi: 10.3866/PKU.WHXB202408015
4. [4]
  Xiaowu Zhang , Pai Liu , Qishen Huang , Shufeng Pang , Zhiming Gao , Yunhong Zhang . Acid-Base Dissociation Equilibrium in Multiphase System: Effect of Gas. University Chemistry, 2024, 39(4): 387-394. doi: 10.3866/PKU.DXHX202310021
5. [5]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403
6. [6]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-. doi: 10.3866/PKU.WHXB202309036
7. [7]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . 液相法制备硫化物固体电解质及其在全固态锂电池中的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2309019-. doi: 10.3866/PKU.WHXB202309019
8. [8]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
9. [9]
  Anqiu LIU , Long LIN , Dezhi ZHANG , Junyu LEI , Kefeng WANG , Wei ZHANG , Junpeng ZHUANG , Haijun HAO . Synthesis, structures, and catalytic activity of aluminum and zinc complexes chelated by 2-((2,6-dimethylphenyl)amino)ethanolate. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 791-798. doi: 10.11862/CJIC.20230424
10. [10]
  Kangmin Wang , Liqiu Wan , Jingyu Wang , Chunlin Zhou , Ke Yang , Liang Zhou , Bijin Li . Multifunctional 2-(2′-hydroxyphenyl)benzoxazoles: Ready synthesis, mechanochromism, fluorescence imaging, and OLEDs. Chinese Chemical Letters, 2024, 35(10): 109554-. doi: 10.1016/j.cclet.2024.109554
11. [11]
  Jiahong ZHENG , Jingyun YANG . Preparation and electrochemical properties of hollow dodecahedral CoNi₂S₄ supported by MnO₂ nanowires. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1881-1891. doi: 10.11862/CJIC.20240170
12. [12]
  Lihui Jiang , Wanrong Dong , Hua Yang , Yongqing Xia , Hongjian Peng , Jun Yuan , Xiaoqian Hu , Zihan Zeng , Yingping Zou , Yiming Luo . Study on Extraction of p-Hydroxyacetophenone. University Chemistry, 2024, 39(11): 259-268. doi: 10.12461/PKU.DXHX202402056
13. [13]
  Fan Wu , Wenchang Tian , Jin Liu , Qiuting Zhang , YanHui Zhong , Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031
14. [14]
  Zhaoyang WANG , Chun YANG , Yaoyao Song , Na HAN , Xiaomeng LIU , Qinglun WANG . Lanthanide(Ⅲ) complexes derived from 4′-(2-pyridyl)-2, 2′∶6′, 2″-terpyridine: Crystal structures, fluorescent and magnetic properties. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1442-1451. doi: 10.11862/CJIC.20240114
15. [15]
  Yue Li , Minghao Fan , Conghui Wang , Yanxun Li , Xiang Yu , Jun Ding , Lei Yan , Lele Qiu , Yongcai Zhang , Longlu Wang . 3D layer-by-layer amorphous MoS_x assembled from [Mo₃S₁₃]^2- clusters for efficient removal of tetracycline: Synergy of adsorption and photo-assisted PMS activation. Chinese Chemical Letters, 2024, 35(9): 109764-. doi: 10.1016/j.cclet.2024.109764
16. [16]
  Shiyan Cheng , Yonghong Ruan , Lei Gong , Yumei Lin . Research Advances in Friedel-Crafts Alkylation Reaction. University Chemistry, 2024, 39(10): 408-415. doi: 10.12461/PKU.DXHX202403024
17. [17]
  Ruolin CHENG , Haoran WANG , Jing REN , Yingying MA , Huagen LIANG . Efficient photocatalytic CO₂ cycloaddition over W₁₈O₄₉/NH₂-UiO-66 composite catalyst. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 523-532. doi: 10.11862/CJIC.20230349
18. [18]
  Liyang ZHANG , Dongdong YANG , Ning LI , Yuanyu YANG , Qi MA . Crystal structures, luminescent properties and Hirshfeld surface analyses of three cadmium(Ⅱ) complexes based on 2-(3-(pyridin-2-yl)-1H-pyrazol-1-yl)benzoate. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1943-1952. doi: 10.11862/CJIC.20240079
19. [19]
  Jianjun LI , Mingjie REN , Lili ZHANG , Lingling ZENG , Huiling WANG , Xiangwu MENG . UV-assisted degradation of tetracycline hydrochloride by MnFe₂O₄@activated carbon activated persulfate. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1869-1880. doi: 10.11862/CJIC.20240187
20. [20]
  Yujia LI , Tianyu WANG , Fuxue WANG , Chongchen WANG . Direct Z-scheme MIL-100(Fe)/BiOBr heterojunctions: Construction and photo-Fenton degradation for sulfamethoxazole. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 481-495. doi: 10.11862/CJIC.20230314

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(280)
HTML views(3)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142