Citation: ZHAO Yue-Liang, WANG Feng, ZHOU Hong-Jie, LI Wei-Ming, LI An, LIU Hai-Jing, HA Yi-Ming. Determination of 2-Dodecylcyclobutanone in Irradiated Beef by Direct Solvent Extraction and Gas Chromatography-Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(12): 1919-1923. doi: 10.3724/SP.J.1096.2012.20428 shu

Determination of 2-Dodecylcyclobutanone in Irradiated Beef by Direct Solvent Extraction and Gas Chromatography-Mass Spectrometry

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Institute of Agro-food Science & Technology Chinese Academy of Agricultural Sciences, Key Opening Laboratory of Agricultural Products Processing and Quality Control, Ministry of Agriculture, Beijing 100193, China

Corresponding author: HA Yi-Ming,
Received Date: 22 April 2012
Available Online: 13 August 2012
Fund Project: 本文系"十二五"国家科技支撑计划(Nos.2012BAK17B13,2012BAD29B03) (Nos.2012BAK17B13,2012BAD29B03)农业部公益性行业科研专项(No.201103007)资助项目 (No.201103007)

A new rapid analytical method based on direct solvent extraction (DSE)/gas chromatography-mass spectrometry (GC-MS) was developed to analyze 2-dodecyclobutanone (2-DCB) to detect γ-ray irradiated beef. A 5 g sample of ground beef patties was homogenized with 7 g of anhydrous sodium sulfate and blended with 150 mL acetonitrile using a blender for 2 min. The extract solvent was carefully transferred to a 500 mL round-bottom flask through filter paper. And the procedure repeated for two times. Then, the extraction solutions were evaporated to dryness using a rotary evaporator. The round-bottom flask was washed with hexane, and the solution was collected into a 50 mL volumetric flask. The 10 mL hexane aliquot was dried to 1 mL via nitrogen stream and then purified with a silica cartridge (1 g, 6 mL). The elution was concentrated to 1 mL with a nitrogen stream, and 1 μL was injected into the gas chromatography-mass spectrometer. The limit of detection and quantitation was 0.004 and 0.01 mg/kg, respectively. The procedure was evaluated with a recovery test in six samples spiked with 2-DCB at 0.01 and 0.5mg/kg of ground beef, resulting in 87.9%-91.6% recoveries with mostly less than 7% RSD. The operation time for sample preparation was about 60 to 90 min. The method was used to detect 2-DCB in ground beef irradiated at 5 targeted absorbed doses of 0.5, 1.0, 3.0, 5.0 and 7.0 kGy and the concentration of 2-DCB increased linearly with dose (y=0.0608x-0.0004, R²=0.9899).
- 2-Dodecyclobutanone,
- Irradiated beef,
- Direct solvent extraction,
- Gas chromatography-mass spectrometry
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