Citation: WANG Yu-Hong, LIU Fang, ZHAO Hui, WANG Jian-Min, WANG Yan, YAN Chao. Development and Evaluation of Micro Evaporative Light Scattering Detector[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(10): 1622-1626. doi: 10.3724/SP.J.1096.2012.20373 shu

Development and Evaluation of Micro Evaporative Light Scattering Detector

WANG Yu-Hong ^1,2,3 ,
LIU Fang ² ,
ZHAO Hui ² ,
WANG Jian-Min ³ ,
WANG Yan ² ,
YAN Chao ^1,2,3,,

1.
¹ Department of Chemistry, East China Normal University, Shanghai 200062, China;

Corresponding author: YAN Chao,
Received Date: 9 April 2012
Available Online: 20 May 2012
Fund Project: 本文系国家重大科学仪器设备开发专项项目(No.2011YQ150072) (No.2011YQ150072) 国家自然科学基金项目(No.21175092) (No.21175092)上海市引进技术的吸收与创新计划项目(No.JJ-YJCX-03-10-2586)资助 (No.JJ-YJCX-03-10-2586)

A novel micro-flow evaporative light scattering detector (μELSD), suitable for capillary liquid chromatography (cLC) system, was constructed and eveluated. The main parameters of detector, such as the aperture of nebulizer, inner diameter of capillary, specification of the evaporative tube and scattering cell, were optimized. Under the optimal experimental conditions:nebulizer 410 μm I.D., nebulized capillary 50 μm I.D.×360 μm O.D., evaporative tube 15 cm×4.6 mm I.D., scattering cell 60 mm×33 mm I.D., the detection limitation of glucose (S/N＞10) was 1 ng with a linear range of 0.01-1 μg. Good reproducibility has been achieved in peak area and peak height with the RSD (n=6) of 0.4% and 0.3% (n=6), respectively. The detector was successfully coupled to a cLC system and applied to the separation and detection of three kinds of sweeteners, in the condition of capillary column ( C₁₈, 250 μm I.D.) and 0.1% (V/V) ammonium formate (pH=4.5): methanol (60:40, V/V) as mobile phase. The cLC-μELSD system has the advantages in universal applicability, high column efficiency, and less solvent and sample consumptions compare with a common HPLC-ELSD system, and is suitable for separation and analysis of real samples.
- Evaporative light scattering detector,
- Capillary liquid chromatography,
- Miniaturization,
- Sweeteners
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