Citation: LI Chun-Mei, JIN Fen, XU Si-Yuan, LIU Jia-Jia, JIN Mao-Jun, SHAO Hua, WANG Jing, YANG Ming-Qi. Determination of Chlormequat in Muscles of Mice by Solid Phase Extraction-Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(12): 1924-1928. doi: 10.3724/SP.J.1096.2012.20371 shu

Determination of Chlormequat in Muscles of Mice by Solid Phase Extraction-Liquid Chromatography-Tandem Mass Spectrometry

LI Chun-Mei ^1,2 ,
JIN Fen ^1,, ,
XU Si-Yuan ¹ ,
LIU Jia-Jia ^1,3 ,
JIN Mao-Jun ¹ ,
SHAO Hua ¹ ,
WANG Jing ¹ ,
YANG Ming-Qi ^2,,

1.
¹ Key Lab of Agro-product Quality and Safety, Institute of Quality Standards & Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, Beijing 100081, China;

Corresponding author: JIN Fen, YANG Ming-Qi,
Received Date: 9 April 2012
Available Online: 17 July 2012
Fund Project: 本文系国家自然基金资助项目(No.21107138) (No.21107138)

A method for the determination of chlormequat (CQ) in muscles of mice using solid phase extraction with liquid chromatography tandem mass spectrometry was developed. CQ was extracted using acetonitrile, cleaned-up by Weak Cation-exchange (WCX) solid phase extraction (SPE) cartridges and then eluted by 3 mL of formic acid-methanol (1:99, V/V) by gravity. The separation was performed on a hydrophilic interaction liquid chromatography (HILIC) column with aqueous solution containing 10 mmol/L ammonium acetate and 0.1% formic acid-acetonitrile (40:60, V/V) as the mobile phase. CQ was determined in the mode of electrospray ionization source (ESI⁺) and multiple-reaction monitoring (MRM). The quantification was carried out by matrix-matched external standard curve. The results showed that the calibration curve was linear in the range of 5.0-500.0 μg/L with the correlation coefficient of 0.9991. The mean recovery of CQ in spiked muscle samples was 73.2%-82.3% and the relative standard deviation (RSD) was less than 9.3% at 10.0, 100.0 and 200.0 μg/kg with three spiked levels (n=7). The limit of quantification (LOQ) for CQ was 10.0 μg/kg. The method was successfully applied to the analysis of trace level of CQ in muscles of mice.
- Liquid chromatography-mass spectrometry,
- Chlormequat,
- Muscles,
- Weak cation exchange solid phase extraction
1. [1]
  1 ZHANG Xi, JIN Fen, QIAN Yong-Zhong, YU Zhi-Yong, WANG Jing. Chinese Journal of Food and Fermentation Industries Editorial Staff, 2008, 34(10): 127-131
2. [2]
  张曦, 金芬, 钱永忠, 于志勇, 王静. 食品与发酵工业, 2008, 34(10): 127-131
3. [3]
  2 Kamel A H, Moreira F T C, Almeida S A A, Sales M G F. Electroanalysis, 2008, 20(2): 194-202
4. [4]
  3 WANG Jin-Hua, LU Xiao-Yu, HUANG Mei, WU Zhuan, MA Gui-Ping, XU Chao-Yi. Chinese J. Anal. Chem., 2007, 35(10): 1509-1512
5. [5]
  王金花, 卢晓宇, 黄梅, 吴瑑, 马贵平, 徐超一. 分析化学, 2007, 35(10): 1509-1512
6. [6]
  4 Hau J, Riediker S, Varga N, Stadler R H. J. Chromatogr. A, 2000, 878(1): 77-86
7. [7]
  5 Esparza X, Moyano E, Galceran M T. J. Chromatogr. A, 2009, 1216(20): 4402-4406
8. [8]
  6 LV Wen-Yuan. Chinese J. Forensic Medicine, 2001, 16(3): 161-162
9. [9]
  吕文渊. 中国法医学杂志, 2001, 16(3): 161-162
10. [10]
  7 WANG Lü-Quan. Chinese Medical J. Communications, 1996, 10(3): 37-38
11. [11]
  王吕泉. 交通医学, 1996, 10(3): 37-38
12. [12]
  8 FANG Ming-Cheng, LI Xue-Feng, ZHANG Zhi-Wei, ZHU Bao-Lun, YANG Qing-Yu. Chinese Journal of Forensic Science and Technology, 2006, 2: 26
13. [13]
  方明成, 李雪枫, 张志威, 朱宝伦, 杨庆雨. 刑事技术, 2006, 2: 26
14. [14]
  9 Torner H, Blottner S, Kuhla S, Langhammer M, Alm H, Tuchscherer A. Reproductive Toxicology, 1999, 13(5): 399-404
15. [15]
  10 Srensen M T, Danielsen V. International J. Andrology, 2006, 29: 129-133
16. [16]
  11 LI Chun-Mei, JIN Fen, YU Hong-Xia, WANG Jiu-Tao, SHI Xiao-Mei, WANG Jing, YANG Ming-Qi. Chinese J. Environment & Health, 2011, 28(8): 667-670
17. [17]
  李春梅, 金芬, 于洪侠, 王久涛, 史晓梅, 王静, 杨鸣琦. 环境与健康杂志, 2011, 28(8): 667-670
18. [18]
  12 Scorecard, the Pollution Information Site, http://scorecard.goodguide.com/health-effects/chemicals-2.tcl?short_hazard_name=endo&all_p=t
19. [19]
  13 ZHANG Xi. Determination of Chlormequat and Mepiquat in Tomatoes and Preliminary Reaserch on the Residue. Chinese Academy of Agricultural Sciences, 2010
20. [20]
  张曦. 番茄中矮壮素和缩节胺的检测方法及其残留的初步研究. 中国农业科学院, 2010
21. [21]
  14 ZHANG Xi, JIN Fen, QIAN Yong-Zhong, YU Zhi-Yong, WANG Jing. Chinese Journal of Science and Technology of Food Industry, 2011, 6: 394-397
22. [22]
  张曦, 金芬, 钱永忠, 于志勇, 王静. 食品工业科技, 2011, 6: 394-397
23. [23]
  15 CUI Hai-Bin, LIU Xiao-Li, XU Rong-Man, LI Xiao-Hong, DING Lin, REN Fei, YANG Yan-Wei. Chinese Journal of Fujian Analysis & Testing, 2009, 18(1): 20-24
24. [24]
  崔海滨, 刘晓丽, 徐榕蔓, 李晓红, 丁琳, 任菲, 杨艳伟. 福建分析测试, 2009, 18(1): 20-24
25. [25]
  16 Alder L, Startin J R. J. AOAC International, 2005, 88(6): 1762-1776
1. [1]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
2. [2]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
3. [3]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . 液相法制备硫化物固体电解质及其在全固态锂电池中的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2309019-. doi: 10.3866/PKU.WHXB202309019
4. [4]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-. doi: 10.3866/PKU.WHXB202309036
5. [5]
  Gaoyan Chen , Chaoyue Wang , Juanjuan Gao , Junke Wang , Yingxiao Zong , Kin Shing Chan . Heart to Heart: Exploring Cardiac CT. University Chemistry, 2024, 39(9): 146-150. doi: 10.12461/PKU.DXHX202402011
6. [6]
  Xiaowu Zhang , Pai Liu , Qishen Huang , Shufeng Pang , Zhiming Gao , Yunhong Zhang . Acid-Base Dissociation Equilibrium in Multiphase System: Effect of Gas. University Chemistry, 2024, 39(4): 387-394. doi: 10.3866/PKU.DXHX202310021
7. [7]
  Chunai Dai , Yongsheng Han , Luting Yan , Zhen Li , Yingze Cao . Ideological and Political Design of Solid-liquid Contact Angle Measurement Experiment. University Chemistry, 2024, 39(2): 28-33. doi: 10.3866/PKU.DXHX202306065
8. [8]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403
9. [9]
  Zhiwen HU , Weixia DONG , Qifu BAO , Ping LI . Low-temperature synthesis of tetragonal BaTiO₃ for piezocatalysis. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 857-866. doi: 10.11862/CJIC.20230462
10. [10]
  Haiyang Zhang , Yanzhao Dong , Haojie Li , Ruili Guo , Zhicheng Zhang , Jiangjiexing Wu . Exploring the Integration of Chemical Engineering Principle Experiment with Cutting-Edge Research Achievements. University Chemistry, 2024, 39(10): 308-313. doi: 10.12461/PKU.DXHX202405035
11. [11]
  Yixuan Zhu , Qingtong Wang , Jin Li , Lin Chen , Junlong Zhao . Blog of Oxytocin. University Chemistry, 2024, 39(9): 134-140. doi: 10.12461/PKU.DXHX202310090
12. [12]
  Feiya Cao , Qixin Wang , Pu Li , Zhirong Xing , Ziyu Song , Heng Zhang , Zhibin Zhou , Wenfang Feng . Magnesium-Ion Conducting Electrolyte Based on Grignard Reaction: Synthesis and Properties. University Chemistry, 2024, 39(3): 359-368. doi: 10.3866/PKU.DXHX202308094
13. [13]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
14. [14]
  Kexin Dong , Chuqi Shen , Ruyu Yan , Yanping Liu , Chunqiang Zhuang , Shijie Li . Integration of Plasmonic Effect and S-Scheme Heterojunction into Ag/Ag₃PO₄/C₃N₅ Photocatalyst for Boosted Photocatalytic Levofloxacin Degradation. Acta Physico-Chimica Sinica, 2024, 40(10): 2310013-. doi: 10.3866/PKU.WHXB202310013
15. [15]
  Aoyu Huang , Jun Xu , Yu Huang , Gui Chu , Mao Wang , Lili Wang , Yongqi Sun , Zhen Jiang , Xiaobo Zhu . Tailoring Electrode-Electrolyte Interfaces via a Simple Slurry Additive for Stable High-Voltage Lithium-Ion Batteries. Acta Physico-Chimica Sinica, 2025, 41(4): 100037-. doi: 10.3866/PKU.WHXB202408007
16. [16]
  Yukun Xing , Xiaoyu Xie , Fangfang Chen . A Sunlit Gift: Vitamin D. University Chemistry, 2024, 39(9): 28-34. doi: 10.12461/PKU.DXHX202402006
17. [17]
  Yaling Chen . Basic Theory and Competitive Exam Analysis of Dynamic Isotope Effect. University Chemistry, 2024, 39(8): 403-410. doi: 10.3866/PKU.DXHX202311093
18. [18]
  Yutong Dong , Huiling Xu , Yucheng Zhao , Zexin Zhang , Ying Wang . The Hidden World of Surface Tension and Droplets. University Chemistry, 2024, 39(6): 357-365. doi: 10.3866/PKU.DXHX202312022
19. [19]
  Xiaoning TANG , Junnan LIU , Xingfu YANG , Jie LEI , Qiuyang LUO , Shu XIA , An XUE . Effect of sodium alginate-sodium carboxymethylcellulose gel layer on the stability of Zn anodes. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1452-1460. doi: 10.11862/CJIC.20240191
20. [20]
  Hao Wu , Zhen Liu , Dachang Bai . ¹H NMR Spectrum of Amide Compounds. University Chemistry, 2024, 39(3): 231-238. doi: 10.3866/PKU.DXHX202309020

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(368)
HTML views(28)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142