Citation: GAO Xue-Jin, LIU Guang-Sheng, CHENG Li, GENG Ling-Xiao, XUE Ji-Xing, JIA Zhi-Yang, QI Yong-Sheng, WANG Pu. Development of Glucose On-line Detection System for Fermentation Process[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(12): 1945-1949. doi: 10.3724/SP.J.1096.2012.20352 shu

Development of Glucose On-line Detection System for Fermentation Process

1.
¹ School of Electronic Information and Control Engineering, Beijing University of Technology, Beijing 100124, China;

Corresponding author: GAO Xue-Jin,
Received Date: 5 April 2012
Available Online: 26 June 2012
Fund Project: 本文系国家自然科学基金(Nos.61174109,60974133) (Nos.61174109,60974133)北京市教委科技计划面上项目(No.KM201210005002)资助项目 (No.KM201210005002)

Due to the high temperature steam sterilization in the fermentation process, traditional "enzyme membrane" glucose biosensors are no longer suitable for online glucose detection. This leads to failure of real-time glucose feeding control, consequently affecting the quality and yields of fermentation. In order to solve this problem, a glucose on-line detection system for monitoring the fermentation process was set up. The problem was solved by using a self-developed "enzyme solution" glucose biosensor which stands high temperature. Another difficult problem about on-line sampling was solved by using a self-developed dialysis sampling system which also stands high temperature. Experimental results show that the sensitivity of the sensor is 0.259 nA/(mg/L), the detection limit is 0.7 mg/L and the RSD is 2.0% at the glucose concentration of 500 mg/L, and that the system responds linearly to the glucose concentration in the range of 0-1000 mg/L. These results were compared with those on a commercial off-line SBA-40E Biosensing analyzer, exhibiting an excellent linear correlation with the linear coefficient close to 1. Glucose concentration was measured on-line in the coli fermentation process with the detection system, and the results exhibit the same variation trend to the commercial off-line SBA-40E Biosensing Analyzer.
- Fermentation process,
- Glucose concentration,
- On-line measurement,
- Enzyme injection,
- Biosensor
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