Citation: FU Hai-Yuan, YUAN Jian, ZHOU Xiao-Ming, SUN Yun-Bo, HUANG Ya-Juan, HOU Li-Ping, SONG Cun-Yan, YANG Bao-An, ZHEN Pei, GAO Rong, MAO Pan-Yong, LIN Li-Zhong, XU Shu-Fang, XIAO Han-Zu, WEI Kai-Hua. Determination of Hepatocellular Carcinoma Serum Polypeptide Based on Antibody Preconcentration Coupled with Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(12): 1803-1806. doi: 10.3724/SP.J.1096.2012.20331 shu

Determination of Hepatocellular Carcinoma Serum Polypeptide Based on Antibody Preconcentration Coupled with Mass Spectrometry

FU Hai-Yuan ^1,2 ,
YUAN Jian ^2,3 ,
ZHOU Xiao-Ming ^2,3 ,
SUN Yun-Bo ^2,3 ,
HUANG Ya-Juan ^2,3 ,
HOU Li-Ping ^2,3 ,
SONG Cun-Yan ^2,3 ,
YANG Bao-An ⁴ ,
ZHEN Pei ^2,3,, ,
GAO Rong ⁵ ,
MAO Pan-Yong ⁵ ,
LIN Li-Zhong ⁶ ,
XU Shu-Fang ⁶ ,
XIAO Han-Zu ⁶ ,
WEI Kai-Hua ^2,3,,

1.
¹ 301 Militry Hospital of China, Beijing 100853, China;

Corresponding author: ZHEN Pei, WEI Kai-Hua,
Received Date: 20 March 2012
Available Online: 22 July 2012
Fund Project: 本文系卫生部传染病重大专项(No.2008ZX10002-016) (No.2008ZX10002-016)863项目(No.2012AA020205)资助 (No.2012AA020205)

Polypeptide antibody pre-concentration method coupled with mass spectrometry detecting was established for the determination of serum polypeptide. Polypeptide was captured by agarose beads followed by binding of specific polyclonal antibodies from serum. After timed washing steps, the remaining bound polypeptides were eluted from the beads and detected by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). We optimized a protein A agarose bead-based platform amenable to high throughput peptide capture followed by mass spectrometry can achieve ion signal, with precision (RSD<12%) and accuracy (about 10% of relative error) sufficient for quantifying biomarkers in the physiologically relevant nmol/L range. The method for the detection of peptide 5 (pep5) in the clinical hepatocellular carcinoma serum had sensitivity of 78.0% and specificity of 90%. The method is appropriate for the detection of low concentration biomarkers in clinical samples, and it will be significant for earlier diagnosis of cancers.
- Anti-peptide antibody,
- Immunoaffinity enrichment,
- Mass spectrometry
1. [1]
  1 Sparbier K, Wenzel T, Dihazi H, Blaschke S, Müller GA, Deelder A, Flad T, Kostrzewa M. Proteomics, 2009, 9(6): 1442-1450
2. [2]
  2 YUAN Jian, SUN Yun-Bo, WEI Kai-Hua. Chinese J. Anal. Chem., 2011, 39(8): 1129-1133
3. [3]
  原剑, 孙云波, 魏开华. 分析化学, 2011,39(8): 1129-1133
4. [4]
  3 Jemal A, Bray F, Center M M, Ferlay J, Ward E, Forman D A. CA: Cancer Journal for Clinicians, 2011, 61(12): 69-90
5. [5]
  4 Carter J D, Labean T H. J. Nucleic Acids. 2011, 2011: 926595. Epub 2011 Oct 9
6. [6]
  5 Cui J, Zhang K, Huang Q, Yu Y, Peng X. Anal. Chim. Acta, 2011, 688(1): 84-89
7. [7]
  6 Whiteaker J R, Zhao L, Zhang H Y, Feng L C, Piening B D, Anderson L, Paulovich A G. Anal. Biochem., 2007, 362(1): 44-54
8. [8]
  7 Stefaniuk P, Cianciara J, Wiercinska-Drapalo A. World J. Gastroenterol., 2010, 16(4): 418-424
9. [9]
  8 Anderson N L, Jackson A, Smith D, Hardie D, Borchers C, Pearson T W. Mol. Cell Proteomics, 2009, 8(5): 995-1005
10. [10]
  9 Ziganshin R, Arapidi G, Azarkin I, Zaryadieva E, Alexeev D, Govorun V, Ivanov V. J. Proteomics, 2011, 74(5): 595-606
11. [11]
  10 Villanueva J, Shaffer D R, Philip J, Chaparro C A, Erdjument-Bromage H, Olshen A B, Fleisher M, Lilja H, Brogi E, Boyd J, Sanchez-Carbayo M, Holland E C, Cordon-Cardo C, Scher H I, Tempst P. J. Clin. Invest., 2006, 116(1): 271-284
1. [1]
  Wei Shao , Wanqun Zhang , Pingping Zhu , Wanqun Hu , Qiang Zhou , Weiwei Li , Kaiping Yang , Xisheng Wang . Design and Practice of Ideological and Political Cases in the Course of Instrument Analysis Experiment: Taking the GC-MS Experiment as an Example. University Chemistry, 2024, 39(2): 147-154. doi: 10.3866/PKU.DXHX202309048
2. [2]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
3. [3]
  Zihan Lin , Wanzhen Lin , Fa-Jie Chen . Electrochemical Modifications of Native Peptides. University Chemistry, 2025, 40(3): 318-327. doi: 10.12461/PKU.DXHX202406089
4. [4]
  Hao Wu , Zhen Liu , Dachang Bai . ¹H NMR Spectrum of Amide Compounds. University Chemistry, 2024, 39(3): 231-238. doi: 10.3866/PKU.DXHX202309020
5. [5]
  Yue-Zhou Zhu , Kun Wang , Shi-Sheng Zheng , Hong-Jia Wang , Jin-Chao Dong , Jian-Feng Li . Application and Development of Electrochemical Spectroscopy Methods. Acta Physico-Chimica Sinica, 2024, 40(3): 2304040-0. doi: 10.3866/PKU.WHXB202304040
6. [6]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-0. doi: 10.3866/PKU.WHXB202309036
7. [7]
  Hanmei Lü , Xin Chen , Qifu Sun , Ning Zhao , Xiangxin Guo . Uniform Garnet Nanoparticle Dispersion in Composite Polymer Electrolytes. Acta Physico-Chimica Sinica, 2024, 40(3): 2305016-0. doi: 10.3866/PKU.WHXB202305016
8. [8]
  Xianyong Lu , Tao Hu . Developing an Innovative Inorganic Chemistry Teaching Model Based on Aerospace Specialty Characteristics. University Chemistry, 2025, 40(7): 127-131. doi: 10.12461/PKU.DXHX202409037
9. [9]
  Xinran Zhang , Siqi Liu , Yichi Chen , Qingli Zou , Qinghong Xu , Yaqin Huang . From Protein to Energy Storage Materials: Edible Gelatin Jelly Electrolyte. University Chemistry, 2025, 40(7): 255-266. doi: 10.12461/PKU.DXHX202408104
10. [10]
  Yan Zhang , Xiaoyan Cao , Yiming Li , Shuwei Xia , Mutai Bao . Comparison of Electrolyte Solutions Section in Physical Chemistry Textbooks at Home and Abroad. University Chemistry, 2025, 40(9): 303-309. doi: 10.12461/PKU.DXHX202502027
11. [11]
  Jiandong Liu , Zhijia Zhang , Kamenskii Mikhail , Volkov Filipp , Eliseeva Svetlana , Jianmin Ma . Research Progress on Cathode Electrolyte Interphase in High-Voltage Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(2): 100011-0. doi: 10.3866/PKU.WHXB202308048
12. [12]
  Zeyu XU , Anlei DANG , Bihua DENG , Xiaoxin ZUO , Yu LU , Ping YANG , Wenzhu YIN . Evaluation of the efficacy of graphene oxide quantum dots as an ovalbumin delivery platform and adjuvant for immune enhancement. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1065-1078. doi: 10.11862/CJIC.20240099
13. [13]
  Zhuoming Liang , Ming Chen , Zhiwen Zheng , Kai Chen . Multidimensional Studies on Ketone-Enol Tautomerism of 1,3-Diketones By ¹H NMR. University Chemistry, 2024, 39(7): 361-367. doi: 10.3866/PKU.DXHX202311029
14. [14]
  Zongyuan Chen , ChunSheng Shi , Yiwen Li , Ganlin Zu , Qiang Jin , Haishan Wang , Fujun Wang , Dekun Yan , Zhijun Guo , Wangsuo Wu . Measurement of Uranium Isotopes in Environmental Water Samples by Alpha-Spectroscopy: Design of an Undergraduate Radiochemistry Experiment. University Chemistry, 2025, 40(4): 353-358. doi: 10.12461/PKU.DXHX202406103
15. [15]
  Lingbang Qiu , Jiangmin Jiang , Libo Wang , Lang Bai , Fei Zhou , Gaoyu Zhou , Quanchao Zhuang , Yanhua Cui . In Situ Electrochemical Impedance Spectroscopy Monitoring of the High-Temperature Double-Discharge Mechanism of Nb₁₂WO₃₃ Cathode Material for Long-Life Thermal Batteries. Acta Physico-Chimica Sinica, 2025, 41(5): 100040-0. doi: 10.1016/j.actphy.2024.100040
16. [16]
  Zhaoxuan ZHU , Lixin WANG , Xiaoning TANG , Long LI , Yan SHI , Jiaojing SHAO . Application of poly(vinyl alcohol) conductive hydrogel electrolytes in zinc ion batteries. Chinese Journal of Inorganic Chemistry, 2025, 41(5): 893-902. doi: 10.11862/CJIC.20240368
17. [17]
  Changsheng An , Tao Liu . Decoding SEI chemistry at the lithium-metal potential. Acta Physico-Chimica Sinica, 2025, 41(9): 100101-0. doi: 10.1016/j.actphy.2025.100101
18. [18]
  Zhi Dou , Huiyu Duan , Yixi Lin , Yinghui Xia , Mingbo Zheng , Zhenming Xu . High-Throughput Screening Lithium Alloy Phases and Investigation of Ion Transport for Solid Electrolyte Interphase Layer. Acta Physico-Chimica Sinica, 2024, 40(3): 2305039-0. doi: 10.3866/PKU.WHXB202305039
19. [19]
  Ke Qiu , Fengmei Wang , Mochou Liao , Kerun Zhu , Jiawei Chen , Wei Zhang , Yongyao Xia , Xiaoli Dong , Fei Wang . A Fumed SiO₂-based Composite Hydrogel Polymer Electrolyte for Near-Neutral Zinc-Air Batteries. Acta Physico-Chimica Sinica, 2024, 40(3): 2304036-0. doi: 10.3866/PKU.WHXB202304036
20. [20]
  Jianan Fang , Youhao Gu , Zexuan Gui , Laiying Zhang , Jiawei Yan , Ruming Yuan , Xiaoming Xu . Experimental Improvement and Expansion of the Electromotive Force Method to Determine the Mean Activity Coefficient of Electrolyte Solution. University Chemistry, 2025, 40(11): 263-271. doi: 10.12461/PKU.DXHX202504055

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(533)
HTML views(46)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142