Citation: YAN Xiao-Fei, LI Yun-Tao, WANG Rong-Hui, LIN Jian-Han, WEN Xin-Hua, WANG Mao-Hua, AN Dong, HAN Wei-Jing, YU Yu-De, LI Yan-Bin. An Impedance Immunosensor for Detection of H5 Subtype Avian Influenza Virus[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(10): 1507-1513. doi: 10.3724/SP.J.1096.2012.20120 shu

An Impedance Immunosensor for Detection of H5 Subtype Avian Influenza Virus

1.
¹ Key Laboratory on Modern Precision Agriculture System Integration Research, Ministry of Education, China Agricultural University, Beijing 100083, China;

Corresponding author: WANG Mao-Hua,
Received Date: 14 February 2012
Available Online: 27 March 2012
Fund Project: 本文系国际科技交流与合作专项(No.2010DFA31000)资助 (No.2010DFA31000)

An impedance immunosensor was developed for the rapid detection of H5 subtype avian influenza virus (AIV). Monoclonal antibodies against AIV H5N1 surface antigen hemagglutinin (HA) were immobilized on the surface of gold interdigitated array microelectrodes through protein A for capturing AIV H5N1 in sample solutions. Electrochemical impedance spectroscopy in the presence of [Fe(CN)₆]^3-/4- as a redox probe was used to describe the surface modification of microelectrodes and the binding of viruses. A linear relationship between the logarithmic value of concentration of AIV H5N1 and the change of electron transfer resistance was found in the concentration range of 2¹-2⁶ HA unit per 50 μL, and its correlation coefficient was 0.9885. The detection limit was 2⁰ HA unit per 50 μL, and the detection time was 1 h. This immunosensor could be used repeatedly with good specificity and high sensitivity, and it is promising for rapid detection of pathogenic microorganisms.
- Impedance immunosensor,
- Avian influenza virus,
- Electrochemical impedance spectroscopy,
- Interdigitated array microelectrodes
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沈阳化工大学材料科学与工程学院沈阳 110142