Citation: GAO Ai, WANG Yu-Ru, HE Xi-Wen, YIN Xue-Bo. An Electrochemical Hydrogen Peroxide Biosensor Based on Polydopamine-Entrapped G-Quadruplex-Hemin DNAzyme[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(10): 1471-1476. doi: 10.3724/SP.J.1096.2012.20095 shu

An Electrochemical Hydrogen Peroxide Biosensor Based on Polydopamine-Entrapped G-Quadruplex-Hemin DNAzyme

1.
State Key Laboratory of Medicinal Chemical Biology and Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, China

Corresponding author: YIN Xue-Bo,
Received Date: 8 February 2012
Available Online: 1 June 2012
Fund Project: 本文系国家自然科学基金(No.21075068) (No.21075068) 973 项目(No.2011CB707703) (No.2011CB707703) 天津市自然科学基金(No.11JCZDJC22200) (No.11JCZDJC22200)

The complex of G-quadruplex-hemin, also named peroxidase-mimicking DNAzyme, shows a catalytic activity towards hydrogen peroxide, which is rarely used as a catalyst in the assembly of electrochemical biosensors. Meanwhile, it is of significance to develop novel method in immobilizing DNA for the purpose of fabrication of DNA-based biosensors. Dopamine can form polydopamine film via self-polymerization. In this study, an electrochemical hydrogen peroxide biosensor was successfully constructed using polydopamine-entrapped G-quadruplex-hemin DNAzyme. The mixture of DNA and hemin achieved the formation of G-quadruplex-hemin complex. After the physical adsorption of DNAzyme on the glassy carbon electrode, a droplet containing 5 g/L dopamine in PBS (pH 8.0) was cast onto the surface. Ambient oxygen in the air oxidized dopamine for the formation of polydopamine to retain DNAzyme. The influence of DNA sequence to electrochemistry was investigated, showing a different phenomenon to optical sensors. The proposed biosensor was sensitive to hydrogen peroxide with the linear range spans the concentration from 10 μmol/L to 1.5 mmol/L and the limit of detection of 2.2 μmol/L. The results demonstrated the possibility of enzyme immobilization on the electrode through polydopamine and the substitution of G-quadruplex-hemin DNAzyme for natural enzyme.
- DNAzyme,
- Dopamine,
- Polydopamine,
- G-Quadruplex,
- Hemin,
- Biosensor
1. [1]
  1 Tan X, Zhang J, Tan S, Zhao D, Huang Z, Mi Y, Huang Z. Electroanal., 2009, 21(13): 1514-1520
2. [2]
  2 Zhou K, Zhu Y, Yang X, Luo J, Li C, Luan S. Electrochim. Acta, 2010, 55(9): 3055-3060
3. [3]
  3 ZHENG Long-Zhen, LI Yin-Di, XIONG Le-Yan, LIU Wen, LIU Qiang, HAN Kui, YANG Shao-Ming, XIA Jian. Chinese J. Anal. Chem., 2012, 40(1): 72-76
4. [4]
  郑龙珍, 李引弟, 熊乐艳, 刘文, 刘强, 韩奎, 杨绍明, 夏坚. 分析化学, 2012, 40(1): 72-76
5. [5]
  4 LIU Yan, NIU Wei-Fen, XU Lan. Chinese J. Anal. Chem., 2011, 39(11): 1676-1681
6. [6]
  刘艳,牛卫芬,徐岚. 分析化学, 2011, 39(11): 1676-1681
7. [7]
  5 You J-M, Jeong Y N, Ahmed M S, Kim S K, Choi H C, Jeon S. Biosens. Bioelectron., 2011, 26(5): 2287-2291
8. [8]
  6 Liu H, Wen M, Zhang F, Liu D, Tian Y. Anal. Methods, 2010, 2(2): 143-148
9. [9]
  7 Guascito M R, Chirizzi D, Malitesta C, Mazzotta E, Siciliano M, Siciliano T, Tepore A, Turco A. Biosens. Bioe-lectron., 2011, 26(8): 3562-3569
10. [10]
  8 Travascio P, Li Y, Sen D. Chem. Biol., 1998, 5(9): 505-517
11. [11]
  9 Li T, Shi L, Wang E, Dong S. Chem. Eur. J., 2009, 15(4): 1036-1042
12. [12]
  10 XU Jing, KONG De-Ming. Chinese J. Anal. Chem., 2012, 40(3): 347-353
13. [13]
  徐静, 孔德明. 分析化学, 2012, 40(3): 347-353
14. [14]
  11 Qiu B, Zheng Z Z, Lu Y J, Lin Z Y, Wong K Y, Chen G N. Chem. Commun., 2011, 47(5): 1437-1439
15. [15]
  12 Pavlov V, Xiao Y, Gill R, Dishon A, Kotler M, Willner I. Anal. Chem., 2004, 76(7): 2152-2156
16. [16]
  13 Lee H, Dellatore S M, Miller W M, Messersmith P B. Science, 2007, 318(5849): 426-430
17. [17]
  14 Yin X B, Liu D Y. J. Chromatogr. A, 2008, 1212(1-2): 130-136
18. [18]
  15 Xing B, Yin X B. PLoS One, 2009, 4(7): e6451
19. [19]
  16 Sundquist W I, Klug A. Nature, 1989, 342(6251): 825-829
20. [20]
  17 Li T, Li B, Dong S. Anal. Bioanal. Chem., 2007, 389(3): 887-893
21. [21]
  18 Li T, Dong S, Wang E. Chem. Asian J., 2009, 4(6): 918-922
22. [22]
  19 Nakayama S, Wang J, Sintim H O. Chem. Eur. J., 2011, 17(20): 5691-5698
23. [23]
  20 Kong D M, Yang W, Wu J, Li C X, Shen H X. Analyst, 2010, 135(2): 321-326
1. [1]
  Jing SU , Bingrong LI , Yiyan BAI , Wenjuan JI , Haiying YANG , Zhefeng Fan . Highly sensitive electrochemical dopamine sensor based on a highly stable In-based metal-organic framework with amino-enriched pores. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1337-1346. doi: 10.11862/CJIC.20230414
2. [2]
  Tiantian MA , Sumei LI , Chengyu ZHANG , Lu XU , Yiyan BAI , Yunlong FU , Wenjuan JI , Haiying YANG . Methyl-functionalized Cd-based metal-organic framework for highly sensitive electrochemical sensing of dopamine. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 725-735. doi: 10.11862/CJIC.20230351
3. [3]
  Peiyu Zhang , Aixin Song , Jingcheng Hao , Jiwei Cui . 高频超声法制备聚多巴胺薄膜综合实验. University Chemistry, 2025, 40(6): 210-214. doi: 10.12461/PKU.DXHX202407081
4. [4]
  Yang Meiqing , Lu Wang , Haozi Lu , Yaocheng Yang , Song Liu . Recent Advances of Functional Nanomaterials for Screen-Printed Photoelectrochemical Biosensors. Acta Physico-Chimica Sinica, 2025, 41(2): 2310046-0. doi: 10.3866/PKU.WHXB202310046
5. [5]
  Qiaoqiao BAI , Anqi ZHOU , Xiaowei LI , Tang LIU , Song LIU . Construction of pressure-temperature dual-functional flexible sensors and applications in biomedicine. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2259-2274. doi: 10.11862/CJIC.20240128
6. [6]
  Ke Zhao , Zhen Liu , Luyao Liu , Changyuan Yu , Jingshun Pan , Xuguang Huang . Functionalized Reflective Structure Fiber-Optic Interferometric Sensor for Trace Detection of Lead Ions. Acta Physico-Chimica Sinica, 2024, 40(4): 2304029-0. doi: 10.3866/PKU.WHXB202304029
7. [7]
  Lin′an CAO , Dengyue MA , Gang XU . Research advances in electrically conductive metal-organic frameworks-based electrochemical sensors. Chinese Journal of Inorganic Chemistry, 2025, 41(10): 1953-1972. doi: 10.11862/CJIC.20250160
8. [8]
  Xingchao Zhao , Xiaoming Li , Ming Liu , Zijin Zhao , Kaixuan Yang , Pengtian Liu , Haolan Zhang , Jintai Li , Xiaoling Ma , Qi Yao , Yanming Sun , Fujun Zhang . Photomultiplication-Type All-Polymer Photodetectors and Their Applications in Photoplethysmography Sensor. Acta Physico-Chimica Sinica, 2025, 41(1): 100007-0. doi: 10.3866/PKU.WHXB202311021
9. [9]
  Jiarong Feng , Yejie Duan , Chu Chu , Dezhen Xie , Qiu'e Cao , Peng Liu . Preparation and Application of a Streptomycin Molecularly Imprinted Electrochemical Sensor: A Suggested Comprehensive Analytical Chemical Experiment. University Chemistry, 2024, 39(8): 295-305. doi: 10.3866/PKU.DXHX202401016
10. [10]
  Liwei Wang , Guangran Ma , Li Wang , Fugang Xu . A Comprehensive Analytical Chemistry Experiment: Colorimetric Detection of Vitamin C Using Nanozyme and Smartphone. University Chemistry, 2024, 39(8): 255-262. doi: 10.3866/PKU.DXHX202312094
11. [11]
  Run Yang , Huajie Pang , Huiping Zang , Ruizhong Zhang , Zhicheng Zhang , Xiyan Li , Libing Zhang . Artificial Intelligence-Enabled DNA Computing: Exploring New Frontiers in Bioinformatics. University Chemistry, 2025, 40(9): 107-117. doi: 10.12461/PKU.DXHX202412135
12. [12]
  Changqing MIAO , Fengjiao CHEN , Wenyu LI , Shujie WEI , Yuqing YAO , Keyi WANG , Ni WANG , Xiaoyan XIN , Ming FANG . Crystal structures, DNA action, and antibacterial activities of three tetranuclear lanthanide-based complexes. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2455-2465. doi: 10.11862/CJIC.20240192
13. [13]
  Yihui Song , Shangshang Qin , Kai Wu , Chengyun Jin , Bin Yu . 生物化学在高水平创新型药学人才培养中的交叉融合应用——以去甲基化酶LSD1抑制剂的活性评价为例. University Chemistry, 2025, 40(6): 341-352. doi: 10.12461/PKU.DXHX202406018
14. [14]
  Chang Liu , Tao Wu , Lijiao Deng , Xuzi Li , Xin Fu , Shuzhen Liao , Wenjie Ma , Guoqiang Zou , Hai Yang . Programmed DNA walkers for biosensors. Chinese Chemical Letters, 2024, 35(9): 109307-. doi: 10.1016/j.cclet.2023.109307
15. [15]
  Cun WANG , Shaohan XU , Yuqian ZHANG , Yaoyao ZHANG , Tao GONG , Rong WEN , Yuhang LIAO , Yanrong REN . Terbium complex electrochemiluminescent emitters: Synthesis and application in the detection of epinephrine. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1351-1360. doi: 10.11862/CJIC.20240427
16. [16]
  Leyuan Sun , Xiaoyu Xie , Fangfang Chen . 敦煌壁画的“DNA变身”. University Chemistry, 2025, 40(8): 211-217. doi: 10.12461/PKU.DXHX202410079
17. [17]
  Quanliang Chen , Zhaohui Zhou . Research on the Active Site of Nitrogenase over Fifty Years. University Chemistry, 2024, 39(7): 287-293. doi: 10.3866/PKU.DXHX202310133
18. [18]
  Zongfei YANG , Xiaosen ZHAO , Jing LI , Wenchang ZHUANG . Research advances in heteropolyoxoniobates. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 465-480. doi: 10.11862/CJIC.20230306
19. [19]
  Min LIU , Huapeng RUAN , Zhongtao FENG , Xue DONG , Haiyan CUI , Xinping WANG . Neutral boron-containing radical dimers. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 123-130. doi: 10.11862/CJIC.20240362
20. [20]
  Jianjun LI , Mingjie REN , Lili ZHANG , Lingling ZENG , Huiling WANG , Xiangwu MENG . UV-assisted degradation of tetracycline hydrochloride by MnFe₂O₄@activated carbon activated persulfate. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1869-1880. doi: 10.11862/CJIC.20240187

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(813)
HTML views(65)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142