Citation: GAO Ai, WANG Yu-Ru, HE Xi-Wen, YIN Xue-Bo. An Electrochemical Hydrogen Peroxide Biosensor Based on Polydopamine-Entrapped G-Quadruplex-Hemin DNAzyme[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(10): 1471-1476. doi: 10.3724/SP.J.1096.2012.20095 shu

An Electrochemical Hydrogen Peroxide Biosensor Based on Polydopamine-Entrapped G-Quadruplex-Hemin DNAzyme

1.
State Key Laboratory of Medicinal Chemical Biology and Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, China

Corresponding author: YIN Xue-Bo,
Received Date: 8 February 2012
Available Online: 1 June 2012
Fund Project: 本文系国家自然科学基金(No.21075068) (No.21075068) 973 项目(No.2011CB707703) (No.2011CB707703) 天津市自然科学基金(No.11JCZDJC22200) (No.11JCZDJC22200)

The complex of G-quadruplex-hemin, also named peroxidase-mimicking DNAzyme, shows a catalytic activity towards hydrogen peroxide, which is rarely used as a catalyst in the assembly of electrochemical biosensors. Meanwhile, it is of significance to develop novel method in immobilizing DNA for the purpose of fabrication of DNA-based biosensors. Dopamine can form polydopamine film via self-polymerization. In this study, an electrochemical hydrogen peroxide biosensor was successfully constructed using polydopamine-entrapped G-quadruplex-hemin DNAzyme. The mixture of DNA and hemin achieved the formation of G-quadruplex-hemin complex. After the physical adsorption of DNAzyme on the glassy carbon electrode, a droplet containing 5 g/L dopamine in PBS (pH 8.0) was cast onto the surface. Ambient oxygen in the air oxidized dopamine for the formation of polydopamine to retain DNAzyme. The influence of DNA sequence to electrochemistry was investigated, showing a different phenomenon to optical sensors. The proposed biosensor was sensitive to hydrogen peroxide with the linear range spans the concentration from 10 μmol/L to 1.5 mmol/L and the limit of detection of 2.2 μmol/L. The results demonstrated the possibility of enzyme immobilization on the electrode through polydopamine and the substitution of G-quadruplex-hemin DNAzyme for natural enzyme.
- DNAzyme,
- Dopamine,
- Polydopamine,
- G-Quadruplex,
- Hemin,
- Biosensor
1. [1]
  1 Tan X, Zhang J, Tan S, Zhao D, Huang Z, Mi Y, Huang Z. Electroanal., 2009, 21(13): 1514-1520
2. [2]
  2 Zhou K, Zhu Y, Yang X, Luo J, Li C, Luan S. Electrochim. Acta, 2010, 55(9): 3055-3060
3. [3]
  3 ZHENG Long-Zhen, LI Yin-Di, XIONG Le-Yan, LIU Wen, LIU Qiang, HAN Kui, YANG Shao-Ming, XIA Jian. Chinese J. Anal. Chem., 2012, 40(1): 72-76
4. [4]
  郑龙珍, 李引弟, 熊乐艳, 刘文, 刘强, 韩奎, 杨绍明, 夏坚. 分析化学, 2012, 40(1): 72-76
5. [5]
  4 LIU Yan, NIU Wei-Fen, XU Lan. Chinese J. Anal. Chem., 2011, 39(11): 1676-1681
6. [6]
  刘艳,牛卫芬,徐岚. 分析化学, 2011, 39(11): 1676-1681
7. [7]
  5 You J-M, Jeong Y N, Ahmed M S, Kim S K, Choi H C, Jeon S. Biosens. Bioelectron., 2011, 26(5): 2287-2291
8. [8]
  6 Liu H, Wen M, Zhang F, Liu D, Tian Y. Anal. Methods, 2010, 2(2): 143-148
9. [9]
  7 Guascito M R, Chirizzi D, Malitesta C, Mazzotta E, Siciliano M, Siciliano T, Tepore A, Turco A. Biosens. Bioe-lectron., 2011, 26(8): 3562-3569
10. [10]
  8 Travascio P, Li Y, Sen D. Chem. Biol., 1998, 5(9): 505-517
11. [11]
  9 Li T, Shi L, Wang E, Dong S. Chem. Eur. J., 2009, 15(4): 1036-1042
12. [12]
  10 XU Jing, KONG De-Ming. Chinese J. Anal. Chem., 2012, 40(3): 347-353
13. [13]
  徐静, 孔德明. 分析化学, 2012, 40(3): 347-353
14. [14]
  11 Qiu B, Zheng Z Z, Lu Y J, Lin Z Y, Wong K Y, Chen G N. Chem. Commun., 2011, 47(5): 1437-1439
15. [15]
  12 Pavlov V, Xiao Y, Gill R, Dishon A, Kotler M, Willner I. Anal. Chem., 2004, 76(7): 2152-2156
16. [16]
  13 Lee H, Dellatore S M, Miller W M, Messersmith P B. Science, 2007, 318(5849): 426-430
17. [17]
  14 Yin X B, Liu D Y. J. Chromatogr. A, 2008, 1212(1-2): 130-136
18. [18]
  15 Xing B, Yin X B. PLoS One, 2009, 4(7): e6451
19. [19]
  16 Sundquist W I, Klug A. Nature, 1989, 342(6251): 825-829
20. [20]
  17 Li T, Li B, Dong S. Anal. Bioanal. Chem., 2007, 389(3): 887-893
21. [21]
  18 Li T, Dong S, Wang E. Chem. Asian J., 2009, 4(6): 918-922
22. [22]
  19 Nakayama S, Wang J, Sintim H O. Chem. Eur. J., 2011, 17(20): 5691-5698
23. [23]
  20 Kong D M, Yang W, Wu J, Li C X, Shen H X. Analyst, 2010, 135(2): 321-326
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沈阳化工大学材料科学与工程学院沈阳 110142