Citation: SHI Jing-Jing, LIAN Jie, ZHOU Wen-Wen, SHI Xi-Zeng, GAO Yun-Hua. Magnetoresistive Biosensor for Detection of Chloramphenicol Residue in Milk[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(10): 1524-1529. doi: 10.3724/SP.J.1096.2012.20046 shu

Magnetoresistive Biosensor for Detection of Chloramphenicol Residue in Milk

1.
¹ Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China;

Corresponding author: GAO Yun-Hua,
Received Date: 13 January 2012
Available Online: 18 April 2012
Fund Project: 本文系国家自然科学基金(No.20975106) (No.20975106) 中国科学院知识创新工程重要方向项目(No.KJCX2-YW-M15) (No.KJCX2-YW-M15)广东省中国科学院全面战略合作项目(No.2011A090100036)资助 (No.2011A090100036)

Based on direct competitive immunoassay principle and giant magnetoresistance (GMR) effect, a novel method for detecting chloramphenicol (CAP) by magnetic biosensor has been developed. The sample, biotinylated anti-CAP monoclonal antibody, and streptavidin-beads conjugate were added onto the prepared CAP hapten chips one by one. Subsequently, competitive immune reaction happened and the number of beads bonded on chips was detected by magnetic biosensor. The standard curve was established by optimizing the detection condition. This method can be used to detect CAP in a range of 0.05-100.0 μg/L, detection limit of 50 ng/L. The analyte recoveries in milk samples ranged from 95.97% to 99.36%. The relative standard deviations ranged from 0.8% to 3.9% for intra-assay and 1.1% to 1.7% for inter-assay. The correlation coefficient between this method and ELISA was 0.98. Rapid quantitative detection could be accomplished within 30 minutes using this method. It provides feasibility for the establishment of rapid multi-target magnetic competitive immunoassay system.
- Magnetic competitive immunoassay,
- Magnetic biosensor,
- Chloramphenicol,
- Number of beads
1. [1]
  1 Maciej J B, Huda H, Eid A E, Zuhour I, Mohammed A T. J. Chromatogr. B, 2004, 807(2): 343-356
2. [2]
  2 Vivekanandan K, Guru S M, Prasad S. Rapid Commun. Mass Spectrom., 2005, 19(21): 3025-3030
3. [3]
  3 Maximum Residue Limit of Veterinary Drug in Animal-Source Food. Attachment of Announcement No.235, Ministry of Agriculture of the People's Republic of China.
4. [4]
  动物源性食品中兽药最高残留限量. 中华人民共和国农业部第235号公告附件
5. [5]
  4 WANG Shi-Cheng, LI Guo-Chen, WANG Yan-Hong, HU Xiao-Yan, ZHANG Hong. Chinese J.Anal.Chem., 2010, 38(8): 1199-1202
6. [6]
  王世成, 李国琛, 王颜红, 胡小燕, 张红. 分析化学, 2010, 38(8): 1199-1202
7. [7]
  5 Epstein R L, Henry C, Holland K P, Dreas J. J. AOAC Int., 1994, 77: 570-576
8. [8]
  6 Brner S, Fry H, Balizs G, Kroker R. J. AOAC Int., 1995, 78: 1153-1160
9. [9]
  7 van Ginkel L A, van Rossum H J, Zoontjes P W, van Blitterswijk H, Ellen G, Vander Heft E, Zomer G. Anal. Chim. Acta, 1990, 237: 61-69
10. [10]
  8 Shen H Y, Jiang H L. Anal. Chim. Acta, 2005, 535(1-2): 33-41
11. [11]
  9 Byzova N A, Zvereva E A, Zherdeva A V, Ereminb S A, Dzantiev B B. Talanta, 2010, 81(3): 843-848
12. [12]
  10 Kima D M, Rahmanb M A, Doa M H, Banc C, Shima Y B. Biosen. Bioelectron., 2010, 25(7): 1781-1788
13. [13]
  11 Xu L, Yu H, Akhras M S, Han S J, Osterfeld S, White R L, Pourmand N, Wang S X. Biosen. Bioelectron., 2008, 24(1): 99-103
14. [14]
  12 GasterR S, Hall D A, Wang S X. Lab Chip, 2011, 11(5): 950-956
15. [15]
  13 WU Ling-Ling, JIANG Nan. Occupation and Health, 2008, 24(5): 428-429
16. [16]
  吴玲玲, 姜楠. 职业与健康, 2008, 24(5): 428-429
17. [17]
  14 LIU Nan, GAO Zhi-Xian, SU Pu, CHAO Fu-Huan, ZHU Mao-Xiang, YANG Zhi-Hua, PAN Xiu-Jie. Bull Acad. Mil. Med. Sci., 2009, 33(1): 41-44, 65
18. [18]
  刘楠, 高志贤, 苏璞, 晁福寰, 朱茂祥, 杨陟华, 潘秀颉. 军事医学科学院院刊, 2009, 33(1): 41-44, 65
19. [19]
  15 WU Hui-Juan, WEI Ling, LIU Qing-Jun, LE Jia-Chang, LI Bao-Ming, ZHANG Xiao-Li, GAO Li-Juan, KANG Zi-Jia, ZHAO Long, QIAN Jia-Lin. Food Science, 2010, 31(8): 167-170
20. [20]
  武会娟, 魏玲, 刘清珺, 乐加昌, 李宝明, 张小莉, 高丽娟, 亢子佳, 赵龙, 钱嘉林. 食品科学, 2010, 31(8): 167-170
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沈阳化工大学材料科学与工程学院沈阳 110142