Citation: BAI Dong, SONG Jian-Nan. Study of Metabolic Biomarkers in Plasma of Patients with Dyslipidemia Based on Gas Chromatography-Mass Spectrometry and Graphical Models[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(10): 1482-1487. doi: 10.3724/SP.J.1096.2012.20012 shu

Study of Metabolic Biomarkers in Plasma of Patients with Dyslipidemia Based on Gas Chromatography-Mass Spectrometry and Graphical Models

BAI Dong ,
SONG Jian-Nan ^,

1.
Institute of Basic Theory, China Academic of Chinese Medicine Sciences, Beijing 100700, China

Corresponding author: SONG Jian-Nan,
Received Date: 6 January 2012
Available Online: 26 March 2012
Fund Project: 本文系国家自然科学基金(No.90709008)资助项目 (No.90709008)

To explore the plasma metabolite profiles in patients with dyslipidemia and seek for the metabolic biomarkers of the disease, the plasma metabolite profiles of 37 patients and 10 healthy people were analyzed by gas chromatography-mass spectrometry (GC-MS). Partial least squares-discriminant analysis (PLS-DA) was employed to perform the pattern recognition analysis of the data. The concentrations in plasma of 9 metabolites were found out with significant differences between patients with dyslipidemia and healthy people (p＜0.05) .They were identified as valine, glycine, alanine, pyroglutamic acid, glucuronic acid, galactose, mannose, linoleic acid and glycerol by searching in NIST database. The concentrations of 8 metabolites in plasma of patients with dyslipidemia were less than those of healthy people, while the glycerol`s was higher. The plasma metabolic biomarkers showed good correlation with the diagnostic criteria of dyslipidemia analyzed graphical models. Compared with healthy people, these 9 metabolites showing abnormal assembling in patients with dyslipidemia, and they might be the diagnostic and prognostic special metabolic biomarkers.
- Dyslipidemia,
- Gas chromatography-mass spectrometry,
- Graphical models,
- Metabolomic
1. [1]
  1 Nicholson J K, Lindon J C, Holmes E. Xenobiotica, 1999, 29 (11): 1181-1189
2. [2]
  2 Brindle J T, Antti H, Holmes E, Tranter G, Nicholson J K, Bethell H W, Clarke S, Schofield P M, McKilligin E, Mosedale D E, Grainger D. J. Nat. Med., 2002, 8 (12): 1439-1444
3. [3]
  3 Cheng L L, Burns M A, Taylor J L, He W, Halpern E F, McDougal W S, Wu C L. Cancer Res., 2005, 65 (8): 3030-3034
4. [4]
  4 Odunsi K, Wollman R M, Ambrosone C B, Hutson A, McCann S E, Tammela J, Geisler J P, Miller G, Sellers T, Cliby W, Qian F, Keitz B, Intengan M, Lele S, Alderfer J L. Int. J. Cancer, 2005, 113(5): 782-788
5. [5]
  5 WANG Guang-Ji, CHA Wei-Bin, HAO Hai-Ping, A Ji-Ye. Chinese Journal of Natural Medicines, 2008, 6(2): 89-97
6. [6]
  王广基, 查伟斌, 郝海平, 阿基业. 中国天然药物, 2008, 6(2): 89-97
7. [7]
  6 SONG Jian-Nan, LIU Jun-Lian, FANG Xiang-Zhong, HU Yuan-Hui, LEI Yan, NIU Xiao-Hong, WU Gang, CHEN Bao-Sheng, MA Ya-Luan, CHEN Bing, JIN Hong. Journal of Chinese Integrative Medicine, 2008, 6(12):1233-1237
8. [8]
  宋剑南, 刘军莲, 房祥忠, 胡元会, 雷燕, 牛晓红, 吴刚, 陈保生, 马雅銮, 陈冰, 金红. 中西医结合学报, 2008, 6(12): 1233-1237
9. [9]
  7 YAN Bei, A Ji-Ye, HAO Hai-Ping, WANG Guang-Ji, ZHU Xuan-Xuan, CHA Wei-Bin, LIU Lin-Sheng, GUAN En-Ze, ZHANG Yin, GU Sheng-Hua, HUANG Qing, ZHEN Yuan-Ting. Science in China(Series C: Life Sciences), 2008, 38(12): 1143-1151
10. [10]
  严蓓, 阿基业, 郝海平, 王广基, 朱萱萱, 查伟斌, 刘林生, 管恩泽, 张颖, 顾胜华, 黄青, 郑媛婷. 中国科学 C 辑:生命科学, 2008, 38(12): 1143-1151
11. [11]
  8 TANG Jia-Hang, YE Xi-Yun, LIU Jiang, LI Ping, ZHANG Qian, HU Jing-Jie. Journal of Shanghai Jiaotong University(Medical Science), 2009, 29(2): 150-153
12. [12]
  唐嘉航, 叶希韵, 刘江, 李萍, 张倩, 胡静婕. 上海交通大学学报(医学版), 2009, 29(2): 150-153
13. [13]
  9 SUN Chang-Hao, ZHOU Xiao-Rong, WU Shu-Ran, LIU Rong. Nutrition Newsletter, 2004, 6(4): 16-21
14. [14]
  孙长颢, 周晓蓉, 五舒然, 刘荣. 营养健康新观察, 2004, 6(4): 16-21
15. [15]
  10 LIN Jian-Hua. Foreign Medical Sciences· Surgery Section, 1992, 19(2): 98-99
16. [16]
  林建华. 国外医学·外科学分册, 1992, 19(2): 98-99 11 YU Xiao-Ming, CHEN Ji-Wei, ZHOU Xue-Feng, SHI Huo-Xi. Journal of Hubei Medical University, 2003, 24(3): 251-253
17. [17]
  余晓明, 陈纪伟, 周雪峰, 史火喜. 武汉大学学报(医学版), 2003, 24(3): 251-253 12 WANG Yong, GUO Shu-Zhen, LI Chun, CHEN Jian-Xin, CHUO Wen-Jing, DONG Fang, WANG Wei, WANG Ying-Feng. Journal of Chinese Integrative Medicine, 2011, 9(2): 158-167
18. [18]
  王勇, 郭淑贞, 李春, 陈建新, 啜文静, 董芳, 王伟, 王英锋. 中西医结合学报, 2011, 9(2): 158-167
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