Citation: LI Peng-Fei, WANG Yan, ZHANG Zheng, TONG Wei-Hang, WU Hua, MA Ping, WANG Jing, LIU Li-Hong. Determination of Immunodepressants and Combined Drugs by Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(10): 1573-1578. doi: 10.3724/SP.J.1096.2012.11135 shu

Determination of Immunodepressants and Combined Drugs by Liquid Chromatography-Tandem Mass Spectrometry

LI Peng-Fei ¹ ,
WANG Yan ² ,
ZHANG Zheng ¹ ,
TONG Wei-Hang ² ,
WU Hua ¹ ,
MA Ping ² ,
WANG Jing ² ,
LIU Li-Hong ^1,,

1.
¹ Pharmacy Department of Beijing Chao-Yang Hospital, Beijing 100043, China;

Corresponding author: LIU Li-Hong,
Received Date: 9 October 2011
Available Online: 3 May 2012
Fund Project: 本文系首都医学发展科研基金(No.2007-1016)资助 (No.2007-1016)

This study aims to develop an LC-MS/MS method for the determination of drugs contained immunodepressants and other drugs in human plasma. The drugs and the internal standard were treated by methanol precipitation to remove protein component from human plasma, and then separated on a Kromasil-C₁₈ column (5 μm, 50 mm×4.6 mm) with a gradient elution. The mobile phase consisted of methanol-acetonitrile-1 mmol/L ammonium acetate was maintained at 35 ℃. The flow rate was 1.1 mL/min and 20 μL aliquot of residues was injected into the LC-MS/MS system. Detection was carried out by multiple reaction monitoring on a 3200 QTrap LC-MS/MS system. The assay was linear over the range of 0.2-1000 μg/L with a lower limit of quantitation of 0.2 μg/L. Intra-and inter-day precisions were both less than 15%. The relative deviation was in the range of -13%-9.33%. The stabilities of the method were good. It is a rapid, sensitive, selective and reliable method for the determination of the drugs which contain immunodepressants and other drugs in human plasma. The assay can be applied for the determination of these drugs in human plasma.
- Liquid chromatography tandem mass spectrometry,
- Immunodepressants,
- Drug combination
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沈阳化工大学材料科学与工程学院沈阳 110142