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Citation: JIA Chunhui, QIU Na, WU Pin, WANG Sheng, TANG Kun, ZHANG Fuli. Novel Water-soluble Asymmetric Pentamethine Cyanine Dyes:Synthesis,Fluorescent Properties and Fluorescent Labeling[J]. Chinese Journal of Applied Chemistry, ;2014, 31(11): 1255-1260. doi: 10.3724/SP.J.1095.2014.30661 shu

Novel Water-soluble Asymmetric Pentamethine Cyanine Dyes:Synthesis,Fluorescent Properties and Fluorescent Labeling

JIA Chunhui ^a,b ,
QIU Na ^a ,
WU Pin ^c ,
WANG Sheng ^b ,
TANG Kun ^a ,
ZHANG Fuli ^a,,

1.
a Research Institute of Environmental Medicine, Medical College of Henan University, Kaifeng 475004, China;
2.
b Pharmaceutical College of Henan University, Kaifeng 475004, China;
3.
c Second Branch of Henan Jinkai Investment Holding Group, Kaifeng 475100, China

Corresponding author: ZHANG Fuli,
Received Date: 26 December 2013
Available Online: 19 March 2014
Fund Project: 国家自然科学基金(21202036)资助项目 (21202036)

Novel asymmetric pentamethine cyanine(Cy5) dye with a branched ether chain(polyethylene glycol) was synthesized.The structure of the product was identified by ¹H NMR and HRMS.The fluorescence properties and light stability of this Cy5 were investigated.Then albumin bovine serum(BSA) was labeled with this Cy5.The maximum absorption and emission wavelengths of the dye in water were 657 nm and 671 nm.The fluorescence quantum yield(Ф) was 0.24 in water.After irradiated for 8 h,the 4.4% of the aqueous soluble dye was photo-degradated.The dye-to-protein(D/P) value of fluorescent labeling reached 1.57 when the molar ratio of dye and BSA was 2:1.
- indocyanine dyes,
- pentamethine,
- fluorescent labeling,
- branched compounds,
- albumin bovine serum,
- fluorescence quantum yield
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