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Citation: GUO Meng, WANG Shumin, WANG Jiali, LI Dan, GAO Qingyu, LI Limin. Spectroscopic and Electrochemical Methods on the Interaction Mechanism of Baicalein with DNA[J]. Chinese Journal of Applied Chemistry, ;2014, 31(1): 114-119. doi: 10.3724/SP.J.1095.2014.30232 shu

Spectroscopic and Electrochemical Methods on the Interaction Mechanism of Baicalein with DNA

GUO Meng ^a,b,, ,
WANG Shumin ^b ,
WANG Jiali ^b ,
LI Dan ^b ,
GAO Qingyu ^a,, ,
LI Limin ^b

1.
a School of Chemical Engineering and Technology, China University of Mining and Technology, Xuzhou 221116, China;
2.
b Chemistry & Chemical and Environmental Engineering College, Weifang University, Weifang 261061, China

Corresponding author: GUO Meng, GAO Qingyu,
Received Date: 13 May 2013
Available Online: 8 June 2013
Fund Project: 山东省自然科学基金资助项目(Y2008B30) (Y2008B30)潍坊市科技发展计划项目(2060402) (2060402)

The interaction between fish sperm DNA(fsDNA) and baicalein(BAI) in NaAc-HAc buffer(pH=3.22) was investigated by using cyclic voltammetry,square wave voltammetry,fluorescence spectroscopy,ultraviolet visible spectroscopy and viscosity measurement.Their interaction is in a groove binding mode and the complex has a higher electrical activity.The peak current of BAI is enhanced significantly because fsDNA provides a low polar hydrophobic environment.The enhanced peak current is in proportional to the concentration of fsDNA in the range of 7.0×10^-8~7.0×10^-6 g/mL.Based on this observstion,a new method with a detection limits at 4.1×10^-8 g/mL for the determination of nucleic acids is established.
- baicalein,
- DNA,
- electrochemistry,
- groove binding mode
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