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Citation: XU Rong, RUAN Lin'gao, GE Mei, ZHANG Yixuan. Synthesis of Fluorescein Isothiocyanate-labeled Vancomycin and Its Bacterial Detection by Fluorescence[J]. Chinese Journal of Applied Chemistry, ;2014, 31(2): 220-224. doi: 10.3724/SP.J.1095.2014.30114 shu

Synthesis of Fluorescein Isothiocyanate-labeled Vancomycin and Its Bacterial Detection by Fluorescence

XU Rong ^a ,
RUAN Lin'gao ^b ,
GE Mei ^c ,
ZHANG Yixuan ^a,,

1.
a Shenyang Pharmaceutical University, Shenyang 110072, China;
2.
b Shanghai Jiaotong University, Shanghai 200240, China;
3.
c Shanghai Laiyi Biological Drug Development Center, Shanghai 200240, China

Corresponding author: ZHANG Yixuan,
Received Date: 7 March 2013
Available Online: 23 April 2013
Fund Project: 国家重大新药创制项目(2012ZX09301002-003) (2012ZX09301002-003)

Fluorescein isothiocyanate(FITC)-labeled vancomycin(FITC-Vanco,FV) was synthesized.After purified by preparative HPLC,the structure of the target compound was indentified by MS.Its combination amount toward bacteria with different concentration was evaluated by the fluorescence intensity of the bacterial suspension.FV synthesized in this study shows specific binding with different bacteria.It shows promising application value in studying bacterial resistance mechanism and bacterial susceptibility to vancomycin.
- vancomycin,
- fluorescein isothiocyanate,
- fluorescent probe,
- bacterial detection
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