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Citation: ZHONG Hui, FANG Zheng, ZOU Baohua, LI Xin, GUO Kai. Immobilized-Lipase(Candida sp. 99-125)-Catalyzed Esterification of Alkyl Oleates in Solvent-free Systems[J]. Chinese Journal of Applied Chemistry, ;2013, 30(9): 1030-1035. doi: 10.3724/SP.J.1095.2013.20496 shu

Immobilized-Lipase(Candida sp. 99-125)-Catalyzed Esterification of Alkyl Oleates in Solvent-free Systems

ZHONG Hui ^a, ,
FANG Zheng ^a ,
ZOU Baohua ^a ,
LI Xin ^b ,
GUO Kai ^b

a. School of Pharmaceutical Sciences, Nanjing University of Technology, Nanjing 211816, China;
b. College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 211816, China

Received Date: 31 October 2012
Revised Date: 19 December 2012

The esterification between oleic acid and alkyl alcohols in solvent-free systems were catalyzed by an immobilized lipase from Candida sp.99-125. The effects of several factors including enzyme concentration,temperature,molar ratio of oleic acid to alkyl alcohols,and structure of alcohols have been also investigated. The results indicated that the reactions catalyzed by lipase at 20℃ with the presence of 3% (mass fraction) lipase,a 1:1 molar ratio of oleic acid to alcohols,afforded products in high yields. Methanol has certain toxicity on the activity of the lipase. The enzyme showed high selectivity to primary alcohols and low selectivity to secondary and tertiary alcohols because of the sterical effect. It showed high activity to long chain fatty acids and low activity to polybasic acids and polyhydric alcohols with branched chain. The lipase showed no appreciable loss in activity after being continuously operated for 5 times. The enzymatic synthesis gave purer products,compared with the conventional chemical system.
- enzymatic catalysis,
- immobilized lipase,
- esterification,
- oleates,
- solvent-free system
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