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Citation: ZENG Weixiu, TIAN Qingqing, ZHAO Xin, CHEN Bo. Preparation and Properties of Cross-linked Angiotensin Converting Enzyme Aggregates[J]. Chinese Journal of Applied Chemistry, ;2013, 30(7): 815-820. doi: 10.3724/SP.J.1095.2013.20412 shu

Preparation and Properties of Cross-linked Angiotensin Converting Enzyme Aggregates

ZENG Weixiu ^a, ,
TIAN Qingqing ^a,b ,
ZHAO Xin ^a ,
CHEN Bo ^a

a. Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research Ministry of Education, Hunan Normal University, Changsha 410081, China;
b. Hunan Traditional Chinese Medical College, Zhuzhou 412008, China

Received Date: 18 September 2012
Revised Date: 22 November 2012

Cross-linked angiotensin converting enzyme aggregates(ACE-CLEAs) were prepared and their characteristics were compared with that of free ACE including optimal temperature and pH,K_m,v_max,thermal and pH stabilities. Based on the relative activity of immobilized enzyme,the optimal preparation conditions should be as follows:precipitating the enzyme with 80% saturated ammonium sulfate for 0.5 h,and then cross-linking it with 0.02%(mass fraction) glutaraldehyde for 1 h. According to the comparison for enzymology property,ACE-CLEAs had better thermal and pH stabilities than free ACE,and the K_m of ACE-CLEAs was close to that of free ACE,suggesting that ACE-CLEAs had an almost equal affinity to enzyme as the free ACE.
- angiotensin converting enzyme,
- cross-linked enzyme aggregates,
- immobilization
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