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Citation: ZHAI Haiyun, LI Jiangmei, CHEN Zuanguang, ZHOU Qing, PAN Yufang. Rapid Determination of Auramine O in Yellow Croaker by Microchip Capillary Electrophoresis[J]. Chinese Journal of Applied Chemistry, ;2013, 30(4): 481-485. doi: 10.3724/SP.J.1095.2013.20273 shu

Rapid Determination of Auramine O in Yellow Croaker by Microchip Capillary Electrophoresis

a. Department of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China;
b. School of Phamaceutical Sciences, Sun Yat-sen University, Guangzhou 510089, China

Received Date: 20 June 2012
Revised Date: 5 September 2012

A method was developed for the rapid determination of auramine O in yellow croaker by microchip capillary electrophoresis with contactless conductivity detection.Effects of the type and concentration of buffer,separation voltage and injection time on the separation and detection were investigated,and the optimal analysis conditions were obtained in the buffer solution of 5.0 mmol/L lactate(pH=3.29) at the separation voltage of 1.8 kV,where auramine O could be separated and determined within 1.0 min.The linear range of the concentration of auramine O is from 5.0 mg/L to 100.0 mg/L,and the limit of detection is 0.2 mg/kg(S/N=3).The method was used for the analysis of auramine O in the yellow croaker satisfactorily.
- microchip capillary electrophoresis,
- contactless conductivity detection,
- auramine O
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