Citation:
WU Fei, ZHU Jin, TAN Kejun. Resonance Light Scattering Spectra of Perfluorooctane Sulfonate-Protein System and Its Analytical Application[J]. Chinese Journal of Applied Chemistry,
;2012, 29(8): 969-973.
doi:
10.3724/SP.J.1095.2012.00387
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The interaction of bovine serum albumin(BSA) with perfluorooctane sulfonate(PFOS) was investigated with resonance light scattering(RLS) spectroscopy.A RLS method for the determination of PFOS was established.In pH=4.1 Britton-Robinson buffer solution,perfluorooctane sulfonate anions reacted with the protonated BSA by electrostatic forces and hydrophobic interactions to form ion-association complexes and resulted in greatly enhanced resonance light scattering signals characterized by a peak at 285.0 nm.The RLS intensity of system was proportional to the content of PFOS in the range of 0.2~25.0 μmol/L,and the limit of detection was 20.0 nmol/L.The optimum reaction conditions and interference of foreign substances were investigated.This RLS method was applied to the determination of PFOS in environmental samples with RSD≤4.4%.
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