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Citation: ZHANG Mei, WANG Li, YUAN Huizhen, LIU Li, SONG Yonghai, YE Shuhong, XIAO Xianping, WANG Jingran, LIU Huiping. Preparation of Acetylcholinesterase/Nafion/Prussian Blue Modified Glassy Carbon Electrode and Its Application in Pesticide Detection[J]. Chinese Journal of Applied Chemistry, ;2011, 28(12): 1429-1435. doi: 10.3724/SP.J.1095.2011.00766 shu

Preparation of Acetylcholinesterase/Nafion/Prussian Blue Modified Glassy Carbon Electrode and Its Application in Pesticide Detection

1.
College of Chemistry & Chemical Engineering, Jiangxi Normal University, Nanchang 330022

Corresponding author: SONG Yonghai,
Received Date: 28 December 2010
Available Online: 10 April 2011
Fund Project: 国家自然科学基金(20905032,21065005) (20905032,21065005)江西省自然科学基金(2008GZH0028) (2008GZH0028)江西省教育厅科技项目(GJJ10389) (GJJ10389)江西师范大学研究生创新基金(YJS2010060)资助项目 (YJS2010060)

This paper reports the preparation of an acetylcholinesterase(AChE)/Nafion/Prussian blue(PB)/glass carbon electrode(GCE) and the electrochemical detection of pesticides such as carbaryl and trichlorfon.Atomic force microscopy and electrochemical technique were used to investigate the construction of the modified electrode and its effects on pesticide detection.Those results indicated that AChE dispersed uniformly in Nafion/PB/GCE.The AChE/Nafion/PB/GCE constructed under optimal conditions showed a good linear relationship between the peak current and the concentration of pesticide in the range of 0.01~0.5 μmol/L & 2.0~10.0 μmol/L for carbaryl and 0.02~1.0 μmol/L & 2.0~8.0 μmol/L for trichlorfon,respectively.The limit of detection is 5.0 nmol/L for carbaryl and 10.0 nmol/L for trichlorfon,respectively.Furthermore the modified electrode was applied in the detection of analogous practical samples.It was found that the asprepared sensor had high detection sensitivity,good reproducibility and anti-interference.
- carbaryl,
- trichlorfon,
- prussian blue,
- Nafion,
- acetylcholinesterase,
- electrochemistry
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