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Citation: XIA Binbin, YANG Ruiqin, WANG Yanji, LIU Jianjun. CdSe Quantum Dots Labeling to Blood Fingerprints on Dark Surfaces and Fluorescent Imaging[J]. Chinese Journal of Applied Chemistry, ;2011, 28(6): 689-694. doi: 10.3724/SP.J.1095.2011.00428 shu

CdSe Quantum Dots Labeling to Blood Fingerprints on Dark Surfaces and Fluorescent Imaging

1.
a Department of Forensic Science, Chinese People's Public Security University, Beijing 100038;
2.
b College of Science, Beijing University of Chemical Technology, Beijing

Corresponding author: YANG Ruiqin,
Received Date: 22 July 2010
Available Online: 6 December 2010
Fund Project: 霍英东基金(111092)资助项目 (111092)

Water-soluble fluorescent CdSe quantum dots(QDs) were synthesized using thiolglycolic acid(TGA) as the stabilizer and characterized by fluorescence spectroscopy.The material exhibited strong visible fluorescence under excitation at 365 nm.QDs were applied to label blood fingerprints deposited on non-porous surfaces.Then fluorescent images of fingerprints were obtained by the charge-coupled device(CCD) camera.The effects of labeling time,blood concentration and stored time of blood fingerprints on imaging were investigated.The results showed that the fluorescent images could be obtained within labeling time of 30 min.CdSe QDs can detect blood fingerprints on different surfaces especially on black surface;1% diluted blood fingerprints also could be labeled.The labeled blood fingerprints have higher and stable fluorescence intensity,weaker background interference and better contrast between fingerprints and substrate.CdSe QDs are promising reagents for blood fingerprints development on non-porous dark surfaces.
- CdSe quantum dots,
- blood fingerprints,
- labeling,
- fluorescent-based imaging
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