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Citation: GUO Meng, YANG Jinghe. A Fluorescent Energy Transfer Method and Its Application in the Detection of Folic Acid[J]. Chinese Journal of Applied Chemistry, ;2011, 28(1): 101-106. doi: 10.3724/SP.J.1095.2011.00174 shu

A Fluorescent Energy Transfer Method and Its Application in the Detection of Folic Acid

GUO Meng ^a,, ,
YANG Jinghe ^b

1.
a School of Chemistry and Chemical Engineering, Weifang University, Weifang 261061;
2.
b School of Chemistry and Chemical Engineering, Shandong University, Ji'nan

Corresponding author: GUO Meng,
Received Date: 26 March 2010
Available Online: 19 May 2010
Fund Project: 国家自然科学基金(20575035) (20575035)潍坊市科技发展计划(2060402)资助项目 (2060402)

The detection of folic acid by a fluorescent energy transfer method has been developed. In the folic acid-KMnO₄-Eu³⁺-H₂O₂ system with the presence of HAc-NaAc buffer solution(pH=4.7,0.2 mol/L), the folic acid can be oxidized by KMnO₄ with the presence of H₂O₂ and converted into the purine of butterfly-6-carboxyl acid, which can coordinate with Eu³⁺ forming a complex. The experimental conditions including the concentration of buffer solution and pH value, choice of oxidant, the temperature and heating time, the concentration of Eu³⁺, etc., were optimized. This system can emit the characteristic fluorescence of Eu³⁺ through an intra-molecule energy transfer process. The fluorescent intensity is in proportional to the concentration of folic acid in the range of 4×10^-8~4×10^-5 mol/L with correlation coefficient of 0.9902. The detection limit is found to be 1.2×10^-8 mol/L. The developed method has been successfully applied to the determination of folic acid in the actual samples.
- folic acid,
- Eu,
- fluorescence
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