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Citation: YE Neng-Sheng, WANG Xiao-Bo, WANG Ji-Fen, GU Xue-Xin. Determination of Cocaine and Its Metabolite Ecgonine Methyl Ester in Human Urine by Gas Chromatography[J]. Chinese Journal of Applied Chemistry, ;2010, 27(10): 1221-1224. doi: 10.3724/SP.J.1095.2010.90847 shu

Determination of Cocaine and Its Metabolite Ecgonine Methyl Ester in Human Urine by Gas Chromatography

a. Department of Chemistry, Capital Normal University, Beijing 100048;
b. Department of Criminal Science and Technology, Chinese People's Public Security University, Beijing 100048

Received Date: 16 December 2009
Revised Date: 1 March 2010

A facile method has been set up for the determination of cocaine (COC) and its metabolite ecgonine methyl ester(EME) in human urine by means of gas chromatography(GC) with a flame ionization detector(FID).The urine samples were prepared by liquid-liquid extraction(LLE). To improve the extraction efficiency,the experimental parameters of LLE,including extraction solvent and its amount,pH value of the urine sample and extraction time were investigated.The optimized conditions are:8.0 mL of chloroformisopropanol(volume ratio 9:1),was used extractant,the pH value of urine sample is around 9.5,the extraction temperature and time are 40℃ and 6 min,respectively.The intra-day precisions of COC and EME were 1.73% and 1.44% while the inter-day precisions of COC and EME were 2.57% and 2.89%,respectively.The limits of detection(LOD) for both were 0.04 mg/L. The derivatization of COC and EME in traditional detection process could be bypassed in this protocol. The results indicate that the developed method is rapid,accurate and sensitive,which can be used for the simultaneous determinations of COC and EME in urine samples for forensic applications.
- liquid-liquid extraction,
- gas chromatography,
- cocaine,
- ecgonine methyl ester,
- urine
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