Citation:
GAO Yan-Sheng, WANG Yuan, DI Jun-Wei. Glucose Biosensor Based on Gold Nanoparticles and Enzyme Embedded in Silica Sol-gel Network[J]. Chinese Journal of Applied Chemistry,
;2010, 27(3): 363-366.
doi:
10.3724/SP.J.1095.2010.90180
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A method for fabrication of glucose electrochemical biosensor based on simultaneously embedding gold nanoparticles and glucose oxidase in silica sol-gel network on gold electrode surface was investigated.The preparation conditions were optimized.The enzyme electrode displayed an excellent electrocatalytic response to glucose.The glucose sensor had a linear range from 0.02 to 2.0 mmol/L and a detection limit of 0.005 mmol/L.It was found that the response current to glucose was decreased by about 8% after storage for 100 days at 4℃.The biosensor exhibited high sensitivity,rapid response and good long-term stability.
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Keywords:
- gold nanoparticle,
- sol-gel,
- glucose,
- electrochemical biosensor
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