Citation: Lin LI, Si-Hua QIAN, Tian-Qi LYU, Yu-Hui WANG, Jian-Ping ZHENG. Recent Progress of Library Construction for Next-generation Sequencing[J]. Chinese Journal of Applied Chemistry, ;2021, 38(1): 11-23. doi: 10.19894/j.issn.1000-0518.200158 shu

Recent Progress of Library Construction for Next-generation Sequencing

Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering (NIMTE), Chinese Academy of Sciences, Ningbo 315201, China

Corresponding author: Yu-Hui WANG, wangyuhui@nimte.ac.cn Jian-Ping ZHENG, zhengjianping@nimte.ac.cn
Received Date: 25 May 2020
Accepted Date: 10 December 2020

Fund Project: the Natural Science Foundation of Zhejiang Province LY20B050003Ningbo 3315 Innovation Team 2019A-14-Cthe Major Science and Technology Projects in Ningbo 2016C50009

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High-throughput next-generation sequencing (NGS) is a revolutionary technology in gene sequencing. It is widely used in various solutions in biomedical-relevant fields. Library preparation with high quality and low-cost is the key for NGS. With the development of such sequencing platforms, different library preparation technologies have been built. However, these technologies also possess their own strengths and weaknesses. In this review, we first summarized various library preparation methods for NGS, then discussed the complete process of library preparation in single cell sequencing. We hope that this review can help researchers on the optimal selection of library preparation strategies and also offer suggestions for the development of new NGS library preparation technologies, especially in the exploitation of homebred kits.
- Next-generation sequencing,
- Single cell sequencing,
- Library preparation
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