快速自成胶的可注射粘性热敏水凝胶的抗菌和促进伤口愈合性能分析

引用本文: 黄童黛, 曹玉玉, 江云静, 翟贝贝, 白荣先, 吴杰, 尤祥宇, 孙红梅. 快速自成胶的可注射粘性热敏水凝胶的抗菌和促进伤口愈合性能分析[J]. 分析化学, 2023, 51(6): 982-993. doi: 10.19756/j.issn.0253-3820.231142 shu

Citation: HUANG Tong-Dai, CAO Yu-Yu, JIANG Yun-Jing, ZHAI Bei-Bei, BAI Rong-Xian, WU Jie, YOU Xiang-Yu, SUN Hong-Mei. Analysis of Antimicrobial and Wound-Healing Properties of Injectable and Adhesive Thermosensitive Hydrogels with Ultrafast Self-gelling[J]. Chinese Journal of Analytical Chemistry, 2023, 51(6): 982-993. doi: 10.19756/j.issn.0253-3820.231142 shu

快速自成胶的可注射粘性热敏水凝胶的抗菌和促进伤口愈合性能分析

湖北工业大学生物工程与食品学院, 发酵工程教育部重点实验室, 国家细胞调控与分子药物“111”引智基地, 工业微生物湖北省重点实验室, 湖北省工业发酵协同创新中心, 武汉 430068

通讯作者: 孙红梅,E-mail:hmsunqi@163.com

基金项目:
湖北工业大学国家“111”细胞调控与分子药学中心合作项目(No.XBTK-2022007)和湖北省教育厅科研计划项目(No.B2017047)资助。

摘要: 开发具有形状适应性和良好抑菌活性的智能水凝胶对于细菌感染创面的治疗非常重要。本研究以生物相容性良好的壳聚糖（CS）、β-甘油磷酸钠（β-GP）和聚多巴胺纳米颗粒（PDA NPs）为原料，制备了具有粘附性和光热抗菌活性的可注射热敏水凝胶。通过在CS上接枝具有贻贝类活性的邻苯二酚基团，提高了水凝胶与组织的粘附性。在生理温度（37℃）下，邻苯二酚改性壳聚糖（CHI-C）、β-GP和PDA NPs经过简单搅拌混匀后，放置1.5 min即可通过氢键和席夫碱键结合形成交联网络。在水凝胶中引入具有高效光热转换能力的PDA NPs不仅赋予水凝胶优异的光热抗菌能力，而且增强了水凝胶网络的力学性能。本研究制备的CHI-C/β-GP/PDA水凝胶具有良好的生物相容性、优异的光热转化能力和极佳的抗菌效果。此水凝胶可在小鼠创面原位形成，这为不规则伤口愈合提供了良好的环境。实验结果表明，制备的智能水凝胶有望成为一种具有良好应用前景的伤口敷料，用于促进细菌感染伤口的愈合。

关键词:

English

Analysis of Antimicrobial and Wound-Healing Properties of Injectable and Adhesive Thermosensitive Hydrogels with Ultrafast Self-gelling

Key Laboratory of Fermentation Engineering(Ministry of Education), National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Key Laboratory of Industrial Microbiology in Hubei, Hubei Province Cooperative Innovation Center for Industrial Fermentation, School of Bioengineering and Food, Hubei University of Technology, Wuhan 430068, China

Corresponding author: SUN Hong-Mei, hmsunqi@163.com

Received Date: 12 April 2023
Revised Date: 05 May 2023
Fund Project:
Supported by the Collaborative Grant-in-Aid of the HBUT National ″111″ Center for Cellular Regulation and Molecular Pharmaceutics (No. XBTK-2022007) and the Scientific Research Program Project of Hubei Provincial Department of Education (No. B2017047).

Abstract: The development of a smart hydrogel platform with shape adaptability and excellent antibacterial activity for treating bacteria-infected wound is highly demanded in practical applications. Herein, an injectable thermosensitive hydrogel with adhesive and photothermal antibacterial activity was prepared by combination of biocompatible chitosan (CS), β-sodium glycerophosphate (β-GP) and polydopamine nanoparticles (PDA NPs) via hydrogen bonding and Schiff base bond. It was noted that catechol groups were grafted on the chitosan backbone to improve the adhesive activity between hydrogel and tissues. Catechol-modified chitosan (CHI-C), β-GP and PDA NPs could form a crosslinked network by simple stirring at physiological temperature within 1.5 min. Furthermore, the introduction of PDA NPs with efficient photothermal conversion ability not only endowed the hydrogel with excellent antibacterial ability, but also enhanced the mechanical property of the hydrogel network. The as-prepared CHI-C/β-GP/PDA hydrogel with good biocompatibility, excellent photothermal conversion ability, and fantastic antibacterial effect could be injected directly in the irregular wounds of mice to form the hydrogel in situ. The experimental results demonstrated that the as-prepared smart hydrogel could be expected to serve as a promising wound to promote bacteria-infected wound healing.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

快速自成胶的可注射粘性热敏水凝胶的抗菌和促进伤口愈合性能分析

通讯作者: 孙红梅,E-mail:hmsunqi@163.com

English

Analysis of Antimicrobial and Wound-Healing Properties of Injectable and Adhesive Thermosensitive Hydrogels with Ultrafast Self-gelling

Corresponding author: SUN Hong-Mei, hmsunqi@163.com

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

快速自成胶的可注射粘性热敏水凝胶的抗菌和促进伤口愈合性能分析

通讯作者: 孙红梅,E-mail:hmsunqi@163.com

English

Analysis of Antimicrobial and Wound-Healing Properties of Injectable and Adhesive Thermosensitive Hydrogels with Ultrafast Self-gelling

Corresponding author: SUN Hong-Mei, hmsunqi@163.com

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs