季铵化磁性壳聚糖复合材料对磷酸化肽的富集研究

引用本文: 周丝, 彭宇, 陈燕萍, 贾琼. 季铵化磁性壳聚糖复合材料对磷酸化肽的富集研究[J]. 分析化学, 2023, 51(6): 1051-1058. doi: 10.19756/j.issn.0253-3820.231052 shu

Citation: ZHOU Si, PENG Yu, CHEN Yan-Ping, JIA Qiong. Enrichment of Phosphopeptides with Quaternarized Magnetic Chitosan Composite[J]. Chinese Journal of Analytical Chemistry, 2023, 51(6): 1051-1058. doi: 10.19756/j.issn.0253-3820.231052 shu

季铵化磁性壳聚糖复合材料对磷酸化肽的富集研究

吉林大学化学学院, 长春 130012

通讯作者: 贾琼,E-mail:jiaqiong@jlu.edu.cn

基金项目:
吉林大学无机合成与制备化学国家重点实验室项目(No.2022-21)资助。

摘要: 蛋白质磷酸化是最重要和最普遍的翻译后修饰之一，蛋白质磷酸化的测定对于全面了解生物过程中的磷酸化途径至关重要。质谱技术是分析蛋白质磷酸化的重要手段，但磷酸化肽固有的低丰度、低电离效率以及与非磷酸化肽共存等特性严重影响质谱对其进行直接分析。为解决此问题，需在质谱分析前对磷酸化肽进行选择性富集。本研究制备了一种基于季铵化磁性壳聚糖的复合材料用于磷酸化肽的富集。此磁性材料具有快速的磁响应性、良好的生物相容性、正电性以及廉价易得等优点。采用β-酪蛋白作为模型蛋白质，结果表明此材料对磷酸化肽具有良好的富集选择性。经过富集后，结合基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）检测手段，方法的检出限为0.4 fmol。本方法被成功用于脱脂牛奶中磷酸化肽的检测，表明其对复杂样品中磷酸化肽的富集和检测具有良好的应用潜力。

关键词:

English

Enrichment of Phosphopeptides with Quaternarized Magnetic Chitosan Composite

College of Chemistry, Jilin University, Changchun 130012, China

Corresponding author: JIA Qiong, jiaqiong@jlu.edu.cn

Received Date: 15 February 2023
Revised Date: 31 March 2023
Fund Project:
Supported by the State Key Laboratory of Inorganic Synthesis & Preparative Chemistry, Jilin University (No. 2022-21).

Abstract: Protein phosphorylation is one of the most important and common post-translational modifications, and the determination of protein phosphorylation is essential for comprehensive understanding of phosphorylation pathways in biological processes. Mass spectrometric technique has become an important method to analyze protein phosphorylation. However, the low abundance, low ionization efficiency of phosphopeptides and their coexistence with non-phosphopeptides seriously affect the direct analysis of phosphopeptides by mass spectrometry. To solve this issue, selective enrichment of phosphopeptides is usually required before the analysis step. Functional magnetic materials have good magnetic responsiveness and can be quickly separated from solution with the help of external magnets. In the present work, a new method based on quaternarized magnetic chitosan composite as the adsorbent was developed for enrichment of phosphopeptides combined with matrixassisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). The magnetic material had the advantages such as rapid magnetic response, good biocompatibility, positive electricity, cheap availability, and good enrichment selectivity for phosphopeptides. The detection limit of β-casein was 0.4 fmol after enrichment. This method was successfully applied to detection of phosphopeptides in actual skim milk samples, indicating that the synthesized magnetic material had high application potential in detection of phosphopeptides in complex samples.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

季铵化磁性壳聚糖复合材料对磷酸化肽的富集研究

通讯作者: 贾琼,E-mail:jiaqiong@jlu.edu.cn

English

Enrichment of Phosphopeptides with Quaternarized Magnetic Chitosan Composite

Corresponding author: JIA Qiong, jiaqiong@jlu.edu.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

季铵化磁性壳聚糖复合材料对磷酸化肽的富集研究

通讯作者: 贾琼,E-mail:jiaqiong@jlu.edu.cn

English

Enrichment of Phosphopeptides with Quaternarized Magnetic Chitosan Composite

Corresponding author: JIA Qiong, jiaqiong@jlu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs