单颗粒电感耦合等离子体飞行时间质谱测定生物样品中掺金属微塑料

引用本文: 杨璞, 方昊, 孟紫薇, 郑令娜, 汪冰, 王萌, 刘广才, 程文播, 丰伟悦. 单颗粒电感耦合等离子体飞行时间质谱测定生物样品中掺金属微塑料[J]. 分析化学, 2023, 51(6): 1059-1065. doi: 10.19756/j.issn.0253-3820.231035 shu

Citation: YANG Pu, FANG Hao, MENG Zi-Wei, ZHENG Ling-Na, WANG Bing, WANG Meng, LIU Guang-Cai, CHENG Wen-Bo, FENG Wei-Yue. Determination of Metal-doped Microplastic in Biological Samples by Single Particle-Inductively Coupled Plasma Time-of-Flight Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2023, 51(6): 1059-1065. doi: 10.19756/j.issn.0253-3820.231035 shu

单颗粒电感耦合等离子体飞行时间质谱测定生物样品中掺金属微塑料

杨璞 ^1,2, ,
方昊 ^1,2, ,
孟紫薇 ^1, ,
郑令娜 ^1, ,
汪冰 ^1, ,
王萌 ^{1,
,} ,
刘广才 ^3, ,
程文播 ^{3,
,} ,
丰伟悦 ^1,

1.
中国科学院高能物理研究所, 中国科学院纳米生物效应与安全性重点实验室, 北京 100049;
2.
中国科学院大学, 北京 100049;
3.
中国科学院苏州医工所天津工程技术研究室, 天津市医用质谱精准诊断企业重点实验室, 天津 300399

通讯作者: 王萌,E-mail:wangmeng@ihep.ac.cn; 程文播,E-mail:chengwb@sibet.ac.cn

基金项目:
广东省基础与应用基础研究基金区域联合基金项目(No.DG2231351B)、环境化学国家重点实验室开放课题项目(No.KF2020-19)和天津市医用质谱精准诊断企业重点实验室2022年度开放课题项目资助。

摘要: 建立了单颗粒电感耦合等离子体飞行时间质谱（SP-ICP-TOF-MS）分析生物样品中掺金属聚苯乙烯颗粒的新方法。采用四甲基氢氧化铵和过氧化氢消解含有聚苯乙烯微塑料颗粒的小鼠肝脏样品；采用离心浓缩的方法消除消解液中的基体，以满足SP-ICP-TOF-MS分析要求。本方法对聚苯乙烯颗粒的回收率为102%±11%，微塑料颗粒中Ce、Eu、Ho和Lu的检出限为0.004~0.026 fg，颗粒数量浓度检出限为37 particles/mL。本方法为研究微塑料的环境污染和毒理提供了新途径。

关键词:

English

Determination of Metal-doped Microplastic in Biological Samples by Single Particle-Inductively Coupled Plasma Time-of-Flight Mass Spectrometry

1.
Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China;
2.
University of Chinese Academy of Sciences, Beijing 100049, China;
3.
Tianjin Key Laboratory of Medical Mass Spectrometry for Accurate Diagnosis, Tianjin Engineering Technology Research Branch, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Tianjin 300399, China

Corresponding author: WANG Meng, ; CHENG Wen-Bo,

Received Date: 04 February 2023
Revised Date: 26 April 2023
Fund Project:
Supported by the Guangdong Basic and Applied Basic Research Fund (No. DG2231351B), the State Key Laboratory of Environmental Chemistry and Ecotoxicology (No. KF2020-19) and the Tianjin Key Laboratory of Medical Mass Spectrometry for Accurate Diagnosis.

Abstract: A new method for analysis of metal-doped polystyrene microplastics in biological samples by single particle-inductively coupled plasma time-of-flight mass spectrometry (SP-ICP-TOF-MS) was developed. Mouse liver samples spiked with the particles were successfully digested using tetramethylammonium hydroxide and hydrogen peroxide. Centrifugal concentration method was used to remove the matrix from the digested solution and to meet the analytical requirements of SP-ICP-TOF-MS. The method achieved recoveries of 102%±11% for microplastic particles. The SP-ICP-TOF-MS achieved detection limits of 0.004-0.026 fg for Ce, Eu, Ho, and Lu in microplastics, and 37 particles/mL for particle number concentration. The developed method provided a new approach to study the environmental impact and toxicology of microplastics.

Key words:

Microplastics
/ Inductively coupled plasma time-of-flight mass spectrometry
/ Single particle analysis

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

单颗粒电感耦合等离子体飞行时间质谱测定生物样品中掺金属微塑料

通讯作者: 王萌,E-mail:wangmeng@ihep.ac.cn; 程文播,E-mail:chengwb@sibet.ac.cn

English

Determination of Metal-doped Microplastic in Biological Samples by Single Particle-Inductively Coupled Plasma Time-of-Flight Mass Spectrometry

Corresponding author: WANG Meng, ; CHENG Wen-Bo,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

单颗粒电感耦合等离子体飞行时间质谱测定生物样品中掺金属微塑料

通讯作者: 王萌,E-mail:wangmeng@ihep.ac.cn; 程文播,E-mail:chengwb@sibet.ac.cn

English

Determination of Metal-doped Microplastic in Biological Samples by Single Particle-Inductively Coupled Plasma Time-of-Flight Mass Spectrometry

Corresponding author: WANG Meng, ; CHENG Wen-Bo,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs