Citation: DENG Bi-Tao, XU Fei, CAO Hui, YUAN Min, YU Jin-Song, YIN Feng-Qin, WU Xiu-Xiu, YE Tai. Construction of Nuclease-Powered DNA Walker and Its Application in Food Safety[J]. Chinese Journal of Analytical Chemistry, ;2023, 51(6): 911-921. doi: 10.19756/j.issn.0253-3820.221589 shu

Construction of Nuclease-Powered DNA Walker and Its Application in Food Safety

Shanghai Engineering Research Center of Food Rapid Detection, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China

Corresponding author: YE Tai, taiye@usst.edu.cn
Received Date: 30 November 2022
Revised Date: 4 March 2023

Fund Project: Supported by the National Natural Science Foundation of China (No. 32172303) and the Shanghai Rising-Star Program (No. 22QA1406500).

The rapid and highly sensitive detection of contaminants in foods is an important strategy to prevent food safety incidents. DNA Walker is a dynamic nano-machine with a programmable structure and function. It can be moved along the designed track interface driven by free energy to carry out continuous mechanical displacement. The nuclease powered DNA walker has excellent signal amplification capabilities and reaction kinetics, and the DNA walker-based biosensor combined with aptamer has been widely used in food safety detection. This review summarized the construction strategies of nuclease powered DNA walker and its application in food safety testing, and its further prospects were also discussed.
- Food safety,
- Nuclease,
- DNA walkers,
- Bio-nano interface,
- Review
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