Citation: LIU Guo-Yong, SHI Yu, SUN Jian, MU Jing. Recent Advances on Fluorescence-based Enzyme Linked Immunosorbent Assay[J]. Chinese Journal of Analytical Chemistry, ;2023, 51(3): 331-339. doi: 10.19756/j.issn.0253-3820.221449 shu

Recent Advances on Fluorescence-based Enzyme Linked Immunosorbent Assay

LIU Guo-Yong ¹ ,
SHI Yu ¹ ,
SUN Jian ^2,, ,
MU Jing ^1,,

1.
Institute of Precision Medicine, Peking University Shenzhen Hospital, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen 518036, China;
2.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Corresponding author: SUN Jian, MU Jing,
Received Date: 14 September 2022
Revised Date: 29 January 2023

Fund Project: Supported by the National Natural Science Foundation of China (No. 32101074), the Development Project of Science and Technology of Jilin Province, China (No. 20200201091JC) and the Scientific Research Foundation of Peking University Shenzhen Hospital (KYQD202100X).

Due to advantages in high-throughput, easy reading, simple operation and low cost, enzyme linked immunosorbent assay (ELISA) plays an important role in environmental monitoring, food safety testing and medical diagnosis. However, the insufficient chromogenic part of ELISA results in high detection limit, which limits the further application of ELISA in analysis and detection. To overcome this issue, the conventional ELISA has been improved by many methods. Among them, the fluorometric method has attracted widespread attention given its high sensitivity, simple operation, and fast response. Recently, a variety of fluorescent materials have been developed to construct different types of ELISA, which promotes the application of ELISA in analytical chemistry and biomedical detection. In this paper, the ELISA constructed by fluorescent materials such as organic small molecules, silicon/carbon nanoparticles, metal nanoclusters, and quantum dots was introduced in detail. Additionally, different enzymes in ELISA, including alkaline phosphatase, horseradish peroxidase and other enzyme as labeling enzymes, were systematically reviewed. Furthermore, the prospects of ELISA based on different fluorescent materials were also discussed.
- Enzyme linked immunosorbent assay,
- Fluorescent detection,
- Enzyme,
- Review
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