氮和磷共掺杂碳纳米粒子荧光猝灭选择性检测亚叶酸钙

引用本文: 李莉, 李猛, 杨明利, 李洪雷, 张焕, 马跃, 林乐儿, 张明. 氮和磷共掺杂碳纳米粒子荧光猝灭选择性检测亚叶酸钙[J]. 分析化学, 2023, 51(2): 296-304. doi: 10.19756/j.issn.0253-3820.221407 shu

Citation: LI Li, LI Meng, YANG Ming-Li, LI Hong-Lei, ZHANG Huan, MA Yue, LIN Le-Er, ZHANG Ming. Selective Detection of Calcium Folinate by Fluorescence Quenching of Nitrogen and Phosphorus Codoped Carbon Nanoparticles[J]. Chinese Journal of Analytical Chemistry, 2023, 51(2): 296-304. doi: 10.19756/j.issn.0253-3820.221407 shu

氮和磷共掺杂碳纳米粒子荧光猝灭选择性检测亚叶酸钙

李莉 ^{1,
,} ,
李猛 ^2, ,
杨明利 ^1, ,
李洪雷 ^1, ,
张焕 ^1, ,
马跃 ^1, ,
林乐儿 ^1, ,
张明 ^{1,
,}

1.
南京医科大学康达学院, 连云港 222000;
2.
连云港市第二人民医院胸外科, 连云港 222000

通讯作者: 李莉,E-mail:lili@njmu.edu.cn; 张明,E-mail:535zhangming@163.com

基金项目:
江苏省高校优秀中青年教师和校长境外研修计划基金项目、江苏高校“青蓝工程”项目(No.KD2021qljs001)、连云港高新区科技计划项目(No.HZ201906)、连云港市“海燕计划”项目(No.2020-QD-005)、江苏省大学生创新创业训练计划项目(Nos.202113980012Y,202113980014Y)和南京医科大学康达学院科研发展基金项目(Nos.KD2020KYJJZD074,KD2021KYJJZD014,KD2021KYRC003,KD2021KYRC015)资助。

摘要: 以磷酸铵（（NH₄）₃PO₄）和抗坏血酸钠为原料，通过一步水热法合成了氮和磷共掺杂碳纳米粒子（N/P-CNPs），量子产率为19%。基于亚叶酸钙（CF）对N/P-CNPs的快速、高选择性猝灭，建立了测定CF的新方法。在最优实验条件下，在0.2~108.5 μmol/L范围内，CF的浓度与N/P-CNPs的荧光猝灭程度（（F₀-F）/F₀，F₀为N/P-CNPs的初始荧光强度，F为加入CF后的荧光强度）呈良好的线性关系，检出限为0.05 μmol/L。经实验验证，CF对N/P-CNPs的荧光猝灭机理为内滤效应和静态猝灭效应。将本方法用于实际样品中CF含量的检测，所得结果与高效液相色谱法测定结果基本一致，表明本方法具有良好的实用性，为检测CF提供了一种新策略。

关键词:

English

Selective Detection of Calcium Folinate by Fluorescence Quenching of Nitrogen and Phosphorus Codoped Carbon Nanoparticles

LI Li ^{1,
,} ,
LI Meng ^2, ,
YANG Ming-Li ^1, ,
LI Hong-Lei ^1, ,
ZHANG Huan ^1, ,
MA Yue ^1, ,
LIN Le-Er ^1, ,
ZHANG Ming ^{1,
,}

1.
Kangda College, Nanjing Medical University, Lianyungang 222000, China;
2.
Department of Thoracic Surgery, The Second People's Hospital of Lianyungang, Lianyungang 222000, China

Corresponding author: LI Li, ; ZHANG Ming,

Received Date: 09 August 2022
Revised Date: 11 December 2022
Fund Project:
Supported by the Jiangsu Overseas Research & Training Program for University Prominent Young & Middle-aged Teachers and Presidents Fund, the Qing Lan Project Fund of Jiangsu Province (No. KD2021qljs001), the Science and Technology Project Fund of Gaoxin District in Lianyungang (No. HZ201906), the Petrel Plan Project Fund of Lianyungang (No. 2020-QD-005), the Innovation and Entrepreneurship Training Program for College Students in Jiangsu Province (Nos. 202113980012Y, 202113980014Y) and the Science and Technology Funds of Kangda College of Nanjing Medical University (Nos. KD2020KYJJZD074, KD2021KYJJZD014, KD2021KYRC003, KD2021KYRC015).

Abstract: Nitrogen and phosphorus codoped carbon nanoparticles (N/P-CNPs) were synthesized by one-step hydrothermal method using ammonium phosphate and sodium ascorbate as raw materials, with a quantum yield of 19%. Based on the rapid and highly selective quenching of N/P-CNPs by calcium folinate (CF), a new method for quantitative determination of CF in CF injection samples was established. Under the optimal experimental conditions, the concentration of CF in the range of 0.2-108.5 μmol/L and the degree of fluorescence quenching of N/P-CNPs ((F₀-F)/F₀, F₀ is the initial fluorescence intensity, F is the fluorescence intensity after adding CF) showed a good linear relationship. The detection limit was 0.05 μmol/L. Besides, it was verified that the fluorescence quenching mechanism of CF on N/P-CNPs was inner filter effect and static quenching effect. The established method was used to detect the content of CF in actual samples, and the obtained results were basically consistent with the results of high performance liquid chromatography, indicating that the method had good practicability and provided a new strategy for the detection of CF.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

氮和磷共掺杂碳纳米粒子荧光猝灭选择性检测亚叶酸钙

通讯作者: 李莉,E-mail:lili@njmu.edu.cn; 张明,E-mail:535zhangming@163.com

English

Selective Detection of Calcium Folinate by Fluorescence Quenching of Nitrogen and Phosphorus Codoped Carbon Nanoparticles

Corresponding author: LI Li, ; ZHANG Ming,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

氮和磷共掺杂碳纳米粒子荧光猝灭选择性检测亚叶酸钙

通讯作者: 李莉,E-mail:lili@njmu.edu.cn; 张明,E-mail:535zhangming@163.com

English

Selective Detection of Calcium Folinate by Fluorescence Quenching of Nitrogen and Phosphorus Codoped Carbon Nanoparticles

Corresponding author: LI Li, ; ZHANG Ming,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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