Citation: SHI Lu-Lu, HONG Huan-Huan, CHEN An-Qi, TANG Yan, ZHANG Tao, WEN Lu-Hong, ZHAO Peng. Automatic in Situ Mass Spectrometry Analysis System Based on Pulsed Direct Current Electrospray Ionization and Its Application[J]. Chinese Journal of Analytical Chemistry, ;2023, 51(3): 348-355. doi: 10.19756/j.issn.0253-3820.221368 shu

Automatic in Situ Mass Spectrometry Analysis System Based on Pulsed Direct Current Electrospray Ionization and Its Application

SHI Lu-Lu ^1,2 ,
HONG Huan-Huan ³ ,
CHEN An-Qi ³ ,
TANG Yan ³ ,
ZHANG Tao ¹ ,
WEN Lu-Hong ^2,3,, ,
ZHAO Peng ^3,,

1.
Shanghai Institute of Technical Physics, Chinese Academy of Sciences, Shanghai 200083, China;
2.
China Innovation Instrument Co. Ltd., Ningbo 315100, China;
3.
The Research Institute of Advanced Technologies, Ningbo University, Ningbo 315211, China

Corresponding author: WEN Lu-Hong, ZHAO Peng,
Received Date: 21 July 2022
Revised Date: 26 November 2022

Fund Project: Supported by the National Key Research and Development Program (No. 2018YFC16000600), the Zhejiang Province Welfare Technology Applied Research Project (No. 2020C02023), the Ningbo City Major Scientific and Technological Program of China (No. 20211ZDYF020179) and the Preferred Postdoctoral Research Projects Foundation of Zhejiang Province (No. ZJ2021003).

Pulsed direct current electrospray ionization mass spectrometry (PDESI-MS) offers many advantages such as in situ, real-time, fast, and microvolume sample analysis. However, the current PDESI-MS system requires manual operation for sample processing and mass spectrometry injection, which have many disadvantages such as cumbersome operation, many steps, large errors and low accuracy. Herein, a high-throughput, fully automated and integrated PDESI-MS system was developed, and an in situ detection for small molecule metabolites in eukaryotes (single bladder cancer cells) and prokaryotes (single colonies of bacteria) was explored. The results showed that the automated PDESI-MS system could effectively detect metabolites of different strains and realize the classification and stereotyping of different types of Escherichia coli. In addition, the picoliter droplet generation technology allowed it to be applied to single cell detection. The automated PDESI-MS system could achieve high precision, high density, high repeatability and high automation for in situ sample extraction, especially in detection and analysis of small samples, and showed great application potential and development prospects in food safety, regenerative medicine, drug screening, clinical treatment and other fields.
- Pulsed direct current electrospray ionization,
- Automation,
- In situ detection,
- Small droplets
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