Citation: LIU Li, XU Bin, SONG Jia-Zhuo, ZHANG Zhi-Min, HONG Li-Hua. Controllable Construction of Biomimetic Chitosan-based Hydrogels and[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(10): 1520-1532. doi: 10.19756/j.issn.0253-3820.221286 shu

Controllable Construction of Biomimetic Chitosan-based Hydrogels and

LIU Li ¹ ,
XU Bin ² ,
SONG Jia-Zhuo ¹ ,
ZHANG Zhi-Min ^1,, ,
HONG Li-Hua ^1,,

1.
School and Hospital of Stomatology, Jilin University, Changchun 130021, China;
2.
College of Chemistry, Jilin University, Changchun 130012, China

Corresponding author: ZHANG Zhi-Min, HONG Li-Hua,
Received Date: 10 June 2022
Revised Date: 28 July 2022

Fund Project: Supported by the Jilin Province Science and Technology Development Plan (No.20200404184YY).

Biomimetic chitosan hydrogels (CS-GNPs) with controllable physical and chemical properties for inducing periodontal tissue regeneration were prepared by performing a nucleophilic substitution reaction on the ester group of the natural protein crosslinker genipine (GNP) with polyglucine (1-4)-2-amino-β-D glucose (chitosan, CS) as the matrix under acidic conditions. The structure and morphology of the materials were characterized, and the mechanical properties, porosity, swelling rate, and degradation rate of the material were detected. The cytotoxicities of the materials were evaluated by the viability detection of mice fibroblasts (L-929) in vitro. The effects of the materials on the biological behavior of pluripotent stem cells were analyzed by observing the proliferation rate of periodontal ligament stem cells (hPDLSCs) and detecting mRNA expression levels of periodontal ligament-related genes PLAP-1, COL1, SCX and POSTN by qPCR. The experimental results showed that CS-GNP with different physicochemical characteristics could be obtained by changing the ratio of GNP. The mechanical signals of the materials affected the biological behaviors of pluripotent stem cells and changing the elastic modulus of the materials could regulate the extension area and proliferation rates of stem cells. When the material's modulus of elasticity was (46.02±15.7) kPa, which was close to the normal periodontal ligament extracellular matrix, the differentiation of hPDLSCs could be induced to the direction of the periodontal ligament. The results verified that the physical signals of the stem cell microenvironment could regulate the fate and differentiation of cells, and the simulation of natural extracellular matrix hardness could greatly regulate the directional differentiation of pluripotent stem cells, providing feasible model materials and systems for analyzing and characterizing the influence of the cell microenvironment on stem cell behavior.
- Bionic hydrogel,
- Controllable architecture,
- Pluripotent stem cells,
- Matrix stiffness,
- Biological behavior
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