Citation: ZHANG Dan-Zhi, YIN Qiang, XU Wen-Jing, FANG Yong, JIAO Zheng-Tong, MA Shi-Chun, LIU Zuo-Jun, ZHANG Li-Gan. Relationship Between Organic Acid Metabolites and Microbial Communities During Anaerobic Digestion of Pig Manure[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(8): 1260-1268. doi: 10.19756/j.issn.0253-3820.221225 shu

Relationship Between Organic Acid Metabolites and Microbial Communities During Anaerobic Digestion of Pig Manure

1.
College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China;
2.
Agricultural Engineering Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230001, China;
3.
Biogas Institute of Ministry of Agriculture and Rural Affairs, Chengdu 610041, China

Corresponding author: LIU Zuo-Jun, ZHANG Li-Gan,
Received Date: 9 May 2022
Revised Date: 29 May 2022

Fund Project: Supported by the Project of Science and Technology Innovation Team in Anhui Academy of Agricultural Sciences (No.2022YL036) and the Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture and Rural Affairs of the People's Republic of China (No.2019011).

The stability of organic acid metabolites and microbial communities structure contributed to improve the efficiency of anaerobic digestion in the large-scale biogas project. To study the relationship between the organic acid metabolites and microbial communities, three stirring speeds including 0, 80 and 200 r/min in a 4-L laboratory fermenter were set to monitor the physical and chemical qualitative changes such as volatile fatty acids during pig manure digestion. Combined with high-throughput sequencing technology, the evolution of microbial diversity and community structure in anaerobic digestion system was organically related to the metabolism of organic acids. The results indicated that, Firmicutes, Bacteroides, Proteobacteria and Euryarchaeota were the main dominant bacterial taxa in the microbial community structure. Firstly, moderate strring could improve the efficiency of anaerobic digestion and the stability of microbial community in the fermentation system in which the removal rate of total solids, volatile solids and chemical oxygen demand reached 79.15%, 63.63% and 87.89%, respectively. Moreover, as the main organic acid metabolites, the concentration of the acetic acid and propionic acid were positively correlated with Bacteroidetes and negatively correlated with Proteobacteria. Secondly, with high-speed stirring measure, the increased abundance of hydrogen-producing acetogens Synergistetes led to an increase in the abundance of Acidobacteria. Meanwhile, the concentration of acetic acid and propionic acid was increased by 21.09% and 6.44% respectively, and the abundance of hydrogenotrophic methanogens Methanocorpusculum was increased by 31.95%. Interestingly, the yield of methane had actually fallen. These data showed that excessive stirring during anaerobic digestion increased the concentrations of acetic acid and propionic acid significantly, and promoted the switchcover of methanogenic pathway from acetic acid pyrolysis pathway to less efficient acetic acid oxidation pathway. The results provided a new perspective for understanding the regulation between organic acid metabolism and methanogenic pathway in anaerobic digestion of pig manure.
- Anaerobic digestion,
- Strring,
- Organic acid metabolites,
- Microbial communities
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