Citation: XIAO Shu-Fen, FANG Zheng, WANG Xu-Jun, YANG Tian-Hui, QING Tai-Ping. Aptamer-Mediated Fluorescent Copper Nanoclusters for Label-free and Rapid Detection of Isocarbophos[J]. Chinese Journal of Analytical Chemistry, ;2023, 51(3): 364-372. doi: 10.19756/j.issn.0253-3820.221207 shu

Aptamer-Mediated Fluorescent Copper Nanoclusters for Label-free and Rapid Detection of Isocarbophos

College of Environment and Resources, Xiangtan University, Xiangtan 411105, China

Corresponding author: QING Tai-Ping, taiping_qing@163.com
Received Date: 27 April 2022
Revised Date: 25 December 2022

Fund Project: Supported by the Hunan Provincial Natural Science Foundation of China (No. 2022JJ30559), the Opening Foundation of State Key Laboratory of Chemo/Biosensing and Chemometrics (No. 2019008) and the Student’s Platform for Innovation and Entrepreneurship Training Program.

Based on the target recognition ability of aptamer and the excellent fluorescence performance of copper nanoclusters (CuNCs), a label-free fluorescent probe was developed for rapid detection of isocarbophos (ISO). In the absence of target molecule, the aptamer DNA and complementary DNA in the solution formed double-stranded DNA, which could mediate the synthesis of fluorescent CuNCs and showed a high fluorescence signal. When ISO was present, it would form aptamer-target complex, and released the complementary DNA. Free single stranded DNA could not mediate the synthesis of CuNCs, resulting in weak fluorescence signal in solution. Under the optimal conditions, the fluorescence inhibition rate of the system showed a linear relationship with the logarithm of concentration of ISO within the concentration range of 0.05-25 mg/L, and the detection limit was 47 μg/L (3σ). The method showed a high selectivity in detection of ISO with little interference. In addition, the probe was successfully used for detection of ISO in water samples with the recoveries of 80.3%-108.0%. These results indicated that the developed method could be used to effectively detect ISO residues in actual samples.
- Fluorescent probe,
- Copper nanoclusters,
- Aptamer,
- Isocarbophos
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