Citation: CHEN Ying, MEI Rong-Chao, WANG Yun-Qing, LIU Wan-Hui, CHEN Ling-Xin. Detection of Single Cell Intracellular Environment by Surface Enhanced Raman Scattering Nanotip[J]. Chinese Journal of Analytical Chemistry, ;2023, 51(3): 356-363. doi: 10.19756/j.issn.0253-3820.221167 shu

Detection of Single Cell Intracellular Environment by Surface Enhanced Raman Scattering Nanotip

CHEN Ying ^1,2 ,
MEI Rong-Chao ² ,
WANG Yun-Qing ^2,3,, ,
LIU Wan-Hui ¹ ,
CHEN Ling-Xin ^2,3,,

1.
Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation, Ministry of Education, School of Pharmacy, Yantai University, Yantai 264005, China;
2.
CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;
3.
Shandong Key Laboratory of Coastal Environmental Processes, Yantai 264003, China

Corresponding author: WANG Yun-Qing, CHEN Ling-Xin,
Received Date: 6 April 2022
Revised Date: 13 October 2022

Fund Project: Supported by the National Natural Foundation of China (Nos. 42076199, 21976209).

Surface-enhanced Raman scattering (SERS) nanotip is a kind of new technique for single cell analysis, which shows excellent application potential in detection of intracellular environment and study of cell physiological function. Due to the small number of noble metal particles that can be loaded on SERS nanotips, screening and modifying nanoparticles with high SERS enhancement ability is the key to ensure their detection sensitivity. In this study, core-satellite Au nanoparticles were prepared, and the single particle signal was significantly higher than that of traditional Au nanospheres and Au nanostars. The particle was coated on the surface of glass capillary with tip diameter of about 200 nm to form SERS nanotips, which further functionalized and modified with target-sensitive Raman reporter molecules, enabling them to detect pH value and O₂ content in micro-area environment. As application performance investigation, SERS nanotips realized pH value and anoxic state monitoring in single HL-7702 cell. This study solved the bottleneck issues such as random aggregation and difficulty in precise localization faced by traditional granular SERS probes for cell analysis, providing a new analytical tool for detection and analysis of single cell environment.
- Surface enhanced Raman scattering,
- Single cell detection,
- Nanotip,
- Cell pH value,
- Cell hypoxia
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