Citation: LIU Kai, CHENG Xiao-Xuan, WAN Jian-Fen, PAN Hua-Ping, YAO Jia-Feng. Development of an Electrical Impedance Sensor for Monitoring Stem Cell Growth[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(8): 1188-1195. doi: 10.19756/j.issn.0253-3820.221116 shu

Development of an Electrical Impedance Sensor for Monitoring Stem Cell Growth

1.
School of Mechanical and Electrical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China;
2.
Jiangning Hospital Affiliated to Nanjing Medical University, Nanjing 211199, China;
3.
Joint Laboratory of Medical-Industrial Integration, Nanjing 211199, China

Corresponding author: YAO Jia-Feng, jiaf.yao@nuaa.edu.cn
Received Date: 9 March 2022
Revised Date: 25 April 2022

Fund Project: Supported by the National Natural Science Foundation of China (No.62071224), the Jiangsu Provincial Key R&D Program Social Development-General Project (No.BE2021618), the Open Project of State Key Laboratory of Precision Testing Technology and Instruments (Tianjin University) (No.pilab2107), and the Medical Engineering of Jiangning Hospital Affiliated to Nanjing Medical University Fusion Laboratory Open Project (No.JNYYZXKY202105).

The electrical properties of stem cells during development were investigated based on bioimpedance spectroscopy. First, a high-precision bioimpedance spectroscopy sensor for monitoring stem cell development process was developed. The sensor adopted an inverted tapered structure to realize the self-adaptation of the detection target size, and the electric field was concentrated and narrowed by the tapered chamber. The electric field in the detection area was homogenized to improve the detection accuracy of stem cells. Then, the structure of the sensor was verified and optimized by numerical simulation method. The results showed that the sensor could avoid the influence of positional changes during the development of stem cells on the detection results, and was more sensitive to the size changes of developing stem cells. The structural parameters were optimized, and the optimal monitoring sensitivity was 0.882. Finally, the proposed method was verified by experiment. Taking zebrafish embryonic stem cells as the monitoring object, the development process was monitored for 30 hours. The experimental results showed that with the development of zebrafish embryos, the impedance value gradually increased, and the relaxation frequency decreased gradually (from 1870 kHz to 481 kHz). The results showed that the sensor and monitoring method designed in this work could achieve high-precision monitoring of the developmental process of stem cells.
- Bioimpedance spectroscopy,
- Stem cell sensors,
- Numerical simulation,
- Electrical characterization
1. [1]
  FU X, HE Q, TAO Y, WANG M, WANG W, WANG Y, YU Q, ZHANG F, ZHANG X, CHEN Y G, GAO D, HU P, HUI L, WANG X, ZENG Y A. Sci. China Life Sci., 2021, 64(12):1998-2029.
2. [2]
  ZHAO Y, WANG M, LIANG F, LI J. Stem Cell Res. Ther., 2021, 12(1):588.
3. [3]
  FORBESL H, ANDREWS M R. Neural Reneger. Res., 2021, 16(4):614-617.
4. [4]
  CHAN H J, YANSHRE E, ROY J, TIPOE G L, FUNG M L, LIM L W. Int. J. Mol. Sci., 2021, 22(18):10151.
5. [5]
  ITO E, IHA K, YOSHIMURA T, NAKAISHI K, WATABE S. Adv. Clin. Chem, 2021, 101:121-133.
6. [6]
  LIN Q, HUANG Z, YE X, YANG B, FANG X, LIU B, CHEN H, KONG J. Talanta, 2021, 225:122090.
7. [7]
  BRAUN A C, CAMPOS F A B, ABDALLAH E A, RUANO A P C, MEDINA T S, TARIKI M S, PINTO F F E, MELLO C A L, CHINEN L T D. Front. Oncol., 2021, 11:622626.
8. [8]
9. [9]
10. [10]
  DETREZ E, KERZERHO V, BELHAJ M M, VERGNET A, VERDAL H, ROUYER T, BONHOMMEAU S, LAMLIH A, JULIEN M, BEN A F, RENOVELL M, BERNARD S, SOULIER F. Aquaculture, 2022, 547:737396.
11. [11]
  RAPOPORT Y, GRIMALSKY V, FEDUN V, AGAPITOV O, BONNELL J, GRYTSAI A, MILINEVSKY G, LIASHCHUK A, ROZHNOI A, SOLOVIEVA M, GULIN A. Ann. Geophys., 2020, 38(1):207-230.
12. [12]
  WANG L, HU S, LIU K, CHEN B, WU H, JIA J, YAO J. Rev. Sci. Instrum., 2020, 91(12):124104.
13. [13]
14. [14]
  VEMBADI A, MENACHERY A, QASAIMEH M A. Front. Bioeng. Biotechnol., 2019, 7:147.
15. [15]
  GAWAD S, SCHILD L, RENAUD P. Lab Chip, 2001, 1(1):76-82.
16. [16]
  DE NINNO A, ERRICO V, BERTANI F R, BUSINARO L, BISEGNA P, CASELLI F. Lab Chip, 2017, 17(6):1158-1166.
17. [17]
  ASAMI K, IRIMAJIRI A. Phys. Med. Biol., 2000, 45(11):3285-3297.
18. [18]
  SONG J H, LEE S M, YOO K H. RSC Adv., 2018, 8(54):31246-31254.
19. [19]
  ZHANG Z Z, ZHENG T Y, ZHU R. Anal. Chem., 2020, 92(18):12579-12587.
20. [20]
  HUA S Z, PENNELL T. Lab Chip, 2009, 9(2):251-256.
21. [21]
  LEI K F, HO Y C, HUANG C H, HUANG C H, PAI P C. Talanta, 2021, 229:122259.
22. [22]
  AMERI S K, SINGH P K, DOKMECI M R, KHADEMHOSSEINI A, XU Q, SONKUSALE S R. Biosens. Bioelectron., 2014, 54:462-467.
23. [23]
  ZHANG F, JIN T, HU Q, HE P. J. Electroanal. Chem., 2018, 823:531-536.
24. [24]
  WAN J, YIN H, LIU K, ZHU C, GUAN X, YAO J. IEEE Sens. J., 2021, 1(1):1-9.
25. [25]
  SUN T, GREEN N G, MORGAN H. Nano, 2008, 3(1):55-63.
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沈阳化工大学材料科学与工程学院沈阳 110142