Citation: FENG Yan-Lin, WANG Jian-Lin, NING Xin, CAO Ji-Min. Au@Ag Core-Shell Nanorods for Surface Enhanced Raman Scattering Imaging of Cancer Cells and in Vivo Cancer Spectroscopic Detection[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(8): 1196-1204. doi: 10.19756/j.issn.0253-3820.221016 shu

Au@Ag Core-Shell Nanorods for Surface Enhanced Raman Scattering Imaging of Cancer Cells and in Vivo Cancer Spectroscopic Detection

Key Laboratory of Cellular Physiology, Ministry of Education, Department of Physiology, Shanxi Medical University, Taiyuan 030001, China

Corresponding author: CAO Ji-Min, caojimin@sxmu.edu.cn
Received Date: 11 January 2022
Revised Date: 13 April 2022

Fund Project: Supported by the National Natural Science Foundation of China (No.22007063) and the Shanxi Medical Key Science and Technology Project Plan, China (No.2020XM01).

Surface enhanced Raman scattering (SERS) is a molecular specific hypersensitive spectroscopy technique. However, the existing SERS are mainly limited to extracorporeal sensing. Silver (Ag) material has become the mostly used SERS substrate because of its excellent surface plasmon resonance (SPR) characteristics. However, poor chemical stability and biocompatibility limit its biomedical applications. Therefore, optimizing the SPR activity and stability of Ag materials has become the research focus to broaden its application in vivo. In this study, Au@Ag core-shell nanorods (Au@Ag NRs) were synthesized by chemical deposition. The detection results of transmission electron microscopy (TEM), scanning transmission electron microscopy (STEM), energy dispersive X-ray element mapping (EDX) and X-ray diffraction (XRD) proved that Au@Ag NRs were successfully synthesized. Then, the near infrared Raman molecules of diethylthioacridine carbonyl iodine (DTTC) and mercaptopolyethylene glycol (PEG-SH) were coupled to Au@Ag NRs (p_DAu@Ag NRs) to evaluate SERS properties both in vitro and in vivo. Ultraviolet visible spectrophotometer (UV-Vis) characterization revealed that p_DAu@Ag NRs had good SPR characteristics in the near infrared region, resulting in good SERS properties in vitro. Inductively coupled plasma optical emission spectroscopy (ICP-OES) showed little dissociation of Ag ions, which was not enough to cause biological toxicity. MTS (3-(4,5-dimethylthiazole-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H tetrazole) test further confirmed that p_DAu@Ag NRs had good biocompatibility, ensuring the safe application in vivo. Finally, p_DAu@Ag NRs was used for Raman imaging of human breast cancer (MCF-7) cells and Raman biosensing in MCF-7 tumor-bearing mice. The results showed that Au@Ag NRs had excellent SERS imaging ability in MCF-7 cells and maintained high SERS activity on the tumor site. This technique may be helpful for early monitoring and spectroscopic diagnosis of breast cancer.
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